##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57139425	34.0	31.0	34.0	31.0	34.0
2	32.73532225	34.0	31.0	34.0	31.0	34.0
3	32.7673875	34.0	31.0	34.0	31.0	34.0
4	36.142597	37.0	37.0	37.0	35.0	37.0
5	36.150214	37.0	37.0	37.0	35.0	37.0
6	36.140603	37.0	37.0	37.0	35.0	37.0
7	36.14480125	37.0	37.0	37.0	35.0	37.0
8	36.126899	37.0	37.0	37.0	35.0	37.0
9	37.89430375	39.0	39.0	39.0	37.0	39.0
10-14	38.17187975	39.4	39.2	39.4	35.4	39.4
15-19	39.347795049999995	41.0	40.0	41.0	37.0	41.0
20-24	39.147844899999996	41.0	39.2	41.0	36.0	41.0
25-29	38.76118145	40.4	38.6	41.0	35.0	41.0
30-34	38.10994505	40.0	37.4	41.0	34.0	41.0
35-39	37.1443315	39.0	35.2	41.0	32.8	41.0
40-44	36.186118050000005	37.0	35.0	40.0	32.0	41.0
45-49	35.2414714	35.0	35.0	39.0	31.0	41.0
50-54	33.89695215	34.8	33.4	36.2	29.0	39.8
55-59	34.12177145	35.0	35.0	35.2	31.0	39.2
60-64	33.771522749999995	35.0	34.8	35.0	30.8	37.2
65-69	33.432742000000005	35.0	34.0	35.0	30.0	36.0
70-74	33.1436809	35.0	34.0	35.0	29.4	35.0
75-79	32.84713824999999	35.0	33.6	35.0	29.0	35.0
80-84	32.5126867	35.0	33.0	35.0	27.8	35.0
85-89	32.194283999999996	35.0	33.0	35.0	27.0	35.0
90-94	31.815596999999997	35.0	33.0	35.0	25.8	35.0
95-99	31.257674149999996	35.0	33.0	35.0	23.8	35.0
100-101	30.199577375	34.0	31.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10118.0
3	1573.0
4	1947.0
5	2123.0
6	2541.0
7	3106.0
8	3701.0
9	4022.0
10	4429.0
11	4719.0
12	4750.0
13	4748.0
14	4819.0
15	4954.0
16	5201.0
17	5685.0
18	6731.0
19	7522.0
20	8932.0
21	10593.0
22	12623.0
23	15391.0
24	18643.0
25	22918.0
26	28081.0
27	33986.0
28	42223.0
29	52826.0
30	65454.0
31	84357.0
32	112137.0
33	158623.0
34	259626.0
35	623705.0
36	1366389.0
37	753661.0
38	205688.0
39	41177.0
40	278.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.6245024983758	22.998712415059856	19.040497415183882	27.33628767138046
2	30.738776993716964	23.63070393386669	22.06444149924139	23.56607757317495
3	29.324700789062234	21.766189902949	21.03684210789979	27.872267200088967
4	31.191595777852637	20.48655627505319	19.42100128858395	28.900846658510225
5	30.7406839656905	19.832334927376362	19.41759318539091	30.009387921542224
6	30.19203982142866	19.055798785181967	18.34538517905969	32.40677621432969
7	32.067302237120835	18.925266242502165	19.142333065068197	29.865098455308807
8	29.647317173107563	19.306641060357656	19.814609721681663	31.23143204485312
9	30.436177534580334	18.30097787680215	19.54413669637732	31.7187078922402
10-14	30.57813989494283	19.258925906560094	19.412794992312556	30.750139206184524
15-19	30.653671135592614	19.141893345380108	19.317574958105382	30.886860560921896
20-24	30.642450388075787	19.1642898023489	19.323429045383705	30.869830764191608
25-29	30.688504416867605	19.251422941615214	19.27382697998566	30.786245661531513
30-34	30.78068653109532	19.131049160784592	19.16143076220731	30.92683354591278
35-39	30.699660145215457	19.179111822346258	19.23861380396939	30.882614228468896
40-44	30.78685672450109	19.201086307777267	19.201216382947706	30.810840584773942
45-49	30.85383324943968	19.09261388700541	19.12057843696075	30.932974426594157
50-54	30.732301558847197	19.17324976047038	19.22051787221362	30.873930808468803
55-59	30.773444806447397	19.225569429092264	19.21390037974449	30.787085384715844
60-64	30.80623249085083	19.15645482445138	19.14130974948326	30.89600293521453
65-69	30.73383	19.212305	19.218284999999998	30.83558
70-74	30.787142754999643	19.265142427998498	19.19340217690762	30.754312640094238
75-79	30.805428107392146	19.166889663098942	19.172570633692768	30.855111595816144
80-84	30.712290449004602	19.240461466214633	19.226781954027537	30.82046613075323
85-89	30.80835204899424	19.28701669556788	19.19156044986387	30.71307080557401
90-94	30.847379251901707	19.17319136110256	19.146650570186992	30.83277881680874
95-99	30.748527613823672	19.24205007488337	19.23076230599366	30.7786600052993
100-101	30.923398057245027	19.344989963186894	19.127001658821364	30.604610320746716
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.96079016975626
2	54.30485456689191
3	57.196967989151204
4	60.09244243636286
5	60.75007188723273
6	62.59881603575834
7	61.93240069242963
8	60.87874921796068
9	62.15488542682053
10-14	61.32827910112736
15-19	61.54053169651451
20-24	61.51228115226739
25-29	61.47475007839912
30-34	61.707520077008105
35-39	61.582274373684356
40-44	61.597697309275034
45-49	61.78680767603384
50-54	61.606232367316
55-59	61.560530191163245
60-64	61.70223542606536
65-69	61.569410000000005
70-74	61.54145539509388
75-79	61.66053970320829
80-84	61.53275657975783
85-89	61.52142285456825
90-94	61.68015806871045
95-99	61.52718761912297
100-101	61.52800837799175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35.0
1	26.0
2	16.0
3	17.5
4	21.0
5	25.5
6	39.5
7	57.0
8	72.5
9	90.0
10	106.0
11	147.0
12	176.5
13	223.5
14	307.5
15	373.5
16	447.5
17	540.5
18	660.0
19	823.5
20	1013.5
21	1222.5
22	1479.5
23	1893.5
24	2391.5
25	2947.5
26	3669.5
27	4547.5
28	5504.0
29	6629.0
30	7937.0
31	9244.0
32	10733.5
33	12282.5
34	13696.5
35	15198.0
36	16664.0
37	17962.0
38	19295.0
39	20303.5
40	21343.5
41	22593.5
42	23572.0
43	24515.5
44	25859.0
45	27429.0
46	29645.0
47	32539.5
48	35714.0
49	39972.0
50	45356.5
51	51598.0
52	58973.5
53	66974.0
54	75393.0
55	84891.5
56	95381.0
57	106892.0
58	118135.5
59	129515.0
60	142158.5
61	154171.0
62	166115.0
63	177652.0
64	187093.0
65	194207.5
66	198475.5
67	198629.5
68	194423.5
69	185661.5
70	172940.5
71	156448.5
72	136703.0
73	115687.5
74	94236.0
75	73736.0
76	54723.0
77	38284.0
78	25369.0
79	15788.5
80	9122.0
81	4974.5
82	2669.0
83	1478.0
84	884.5
85	627.0
86	489.5
87	376.5
88	314.0
89	266.0
90	233.5
91	190.0
92	150.0
93	129.0
94	126.5
95	111.0
96	83.5
97	60.0
98	38.0
99	35.0
100	31.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.220175
2	0.195525
3	0.19165000000000001
4	0.18112499999999998
5	0.19090000000000001
6	0.17435
7	0.192025
8	0.18085
9	0.1817
10-14	0.139135
15-19	0.10053
20-24	0.07493000000000001
25-29	0.06266000000000001
30-34	0.10401500000000001
35-39	0.07055499999999999
40-44	0.05779
45-49	0.05203
50-54	0.048895
55-59	0.07755000000000001
60-64	4.95E-4
65-69	0.0
70-74	3.5E-4
75-79	0.017085
80-84	0.032985
85-89	0.001305
90-94	0.00298
95-99	0.02453
100-101	0.0118375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.42909236225585917
#Duplication Level	Relative count
1	100.0
2	0.3135488208055947
3	0.009531884152490079
4	0.0015050343398668546
5	0.0035117467930226606
6	0.002006712453155806
7	0.0015050343398668546
8	0.0
9	5.016781132889515E-4
10++	0.0035117467930226606
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	379270	3.6454704	4.094483	60-64
TTTTT	373985	3.6371243	4.046886	10-14
>>END_MODULE