##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R1_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7531315	34.0	31.0	34.0	31.0	34.0
2	33.00203475	34.0	31.0	34.0	31.0	34.0
3	33.0424925	34.0	31.0	34.0	31.0	34.0
4	36.4565445	37.0	37.0	37.0	35.0	37.0
5	36.3901955	37.0	37.0	37.0	35.0	37.0
6	36.359126	37.0	37.0	37.0	35.0	37.0
7	36.3492185	37.0	37.0	37.0	35.0	37.0
8	36.3443905	37.0	37.0	37.0	35.0	37.0
9	38.13015	39.0	39.0	39.0	37.0	39.0
10-14	38.33990884999999	39.4	38.6	39.4	36.2	39.4
15-19	39.531633299999996	41.0	40.0	41.0	37.0	41.0
20-24	38.6480094	40.4	38.6	41.0	34.0	41.0
25-29	26.14104425	27.2	24.6	30.4	19.4	31.4
30-34	33.172986349999995	37.0	31.6	39.0	17.8	39.8
35-39	34.576908100000004	37.0	33.0	40.0	24.6	41.0
40-44	33.91282845	35.2	32.8	39.2	24.2	40.8
45-49	33.7365629	35.0	33.2	38.2	25.0	40.6
50-54	33.3876102	35.0	33.6	36.2	26.4	39.6
55-59	33.138683799999995	35.0	33.0	35.0	26.6	38.6
60-64	32.707085750000005	35.0	33.0	35.0	26.4	36.6
65-69	32.2892893	35.0	32.6	35.0	26.0	35.4
70-74	31.6786646	35.0	32.6	35.0	24.2	35.0
75-79	31.2909853	34.4	31.6	35.0	23.4	35.0
80-84	31.2356001	35.0	32.4	35.0	23.0	35.0
85-89	30.80982345	34.6	31.6	35.0	21.2	35.0
90-94	30.733957300000004	34.6	31.2	35.0	21.2	35.0
95-99	30.156128250000002	34.0	31.0	35.0	13.6	35.0
100-101	28.562899375	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	16.0
4	57.0
5	159.0
6	604.0
7	2414.0
8	6805.0
9	8304.0
10	7536.0
11	7442.0
12	6471.0
13	7133.0
14	7784.0
15	8623.0
16	9116.0
17	10453.0
18	11414.0
19	12281.0
20	13837.0
21	15800.0
22	19330.0
23	23405.0
24	29849.0
25	37421.0
26	46815.0
27	59534.0
28	75621.0
29	97505.0
30	131444.0
31	191754.0
32	302658.0
33	491584.0
34	770476.0
35	929341.0
36	496071.0
37	135982.0
38	24413.0
39	519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.52596868342954	22.59185083166437	19.33271849861662	27.54946198628947
2	30.474800000000002	22.94895	22.81075	23.7655
3	28.7273	21.753925	21.978125000000002	27.54065
4	30.639699999999998	20.69545	20.195025	28.469825
5	30.803324999999997	19.7572	19.51565	29.923824999999997
6	30.009524999999996	19.266849999999998	18.66425	32.059375
7	31.87895	19.090175000000002	19.235825000000002	29.79505
8	29.735574999999997	19.300775	19.787775	31.175874999999998
9	30.2419	18.43365	19.781075	31.543375
10-14	30.617135	19.30894	19.43893	30.634994999999996
15-19	30.61929	19.192709999999998	19.354955	30.833045
20-24	31.177635401048185	19.091291415227214	19.200698171205648	30.530375012518952
25-29	39.62322739464913	17.0749531220504	16.672748706183143	26.629070777117335
30-34	32.58702629559856	19.134692344714768	18.624924199138277	29.65335716054839
35-39	31.511102661334505	19.1538795853814	19.05881459671232	30.276203156571775
40-44	31.413196216897614	19.141229218765226	18.95548320988184	30.490091354455316
45-49	31.30759	19.356835	19.01288	30.322695
50-54	31.111142777856944	19.41574853937135	19.069982674956687	30.40312600781502
55-59	30.907801631915632	19.261031450558505	19.172921146577956	30.65824577094791
60-64	30.87691937847587	18.947748367102083	19.112521270015375	31.062810984406674
65-69	30.876541675820977	19.300966895214266	19.210799444378413	30.611691984586344
70-74	31.01313	19.30764	19.131035	30.548195
75-79	31.103534999999997	19.19293	19.092624999999998	30.610910000000004
80-84	30.920749693026433	19.31083615564341	19.193661931512633	30.574752219817526
85-89	31.002832448638017	19.299974049515363	19.159729408810552	30.537464093036064
90-94	30.91754648515549	19.13166437989907	19.139719432027768	30.81106970291767
95-99	30.92412093473221	19.145770875465676	19.16468136524736	30.765426824554755
100-101	31.279012499999997	19.145725	18.9845125	30.59075
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.07543066971901
2	54.2403
3	56.26795
4	59.109525
5	60.727149999999995
6	62.068900000000006
7	61.67399999999999
8	60.91145
9	61.785275
10-14	61.25213000000001
15-19	61.452335000000005
20-24	61.70801041356714
25-29	66.25229817176647
30-34	62.24038345614695
35-39	61.78730581790628
40-44	61.90328757135294
45-49	61.63028499999999
50-54	61.514268785671966
55-59	61.566047402863546
60-64	61.93973036288254
65-69	61.48823366040732
70-74	61.561325
75-79	61.714445
80-84	61.495501912843956
85-89	61.54029654167408
90-94	61.72861618807316
95-99	61.68954775928697
100-101	61.8697625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.5
2	7.5
3	8.5
4	11.0
5	11.0
6	13.0
7	22.5
8	30.0
9	39.5
10	46.0
11	58.0
12	76.5
13	92.0
14	127.5
15	179.0
16	234.5
17	302.0
18	386.0
19	490.5
20	650.0
21	809.5
22	1023.5
23	1355.0
24	1736.0
25	2236.5
26	2901.5
27	3657.5
28	4570.0
29	5615.0
30	6700.0
31	8055.5
32	9486.5
33	10969.5
34	12573.0
35	13986.0
36	15530.0
37	17129.5
38	18544.0
39	19944.0
40	21028.5
41	22203.0
42	23402.5
43	24479.0
44	25905.0
45	27673.0
46	29816.5
47	32192.5
48	35382.0
49	39751.0
50	45065.0
51	51343.5
52	58577.0
53	66619.5
54	75440.5
55	85317.0
56	96478.0
57	107480.5
58	118905.0
59	131125.0
60	143553.5
61	156187.5
62	168629.5
63	179771.5
64	188780.0
65	196065.0
66	199981.5
67	200284.5
68	195826.5
69	186522.5
70	173169.0
71	156243.5
72	136498.5
73	115167.5
74	93653.0
75	72892.5
76	54174.0
77	38177.5
78	25719.5
79	16326.0
80	9805.0
81	5695.5
82	3224.0
83	1893.0
84	1207.0
85	821.0
86	566.0
87	421.0
88	304.5
89	207.5
90	145.0
91	102.5
92	75.0
93	49.0
94	25.5
95	16.0
96	11.5
97	5.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.25E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.001605
25-29	3.7740549999999997
30-34	0.091835
35-39	0.036805
40-44	0.003235
45-49	0.0
50-54	2.5E-4
55-59	3.45E-4
60-64	0.04121
65-69	0.047080000000000004
70-74	0.0
75-79	0.0
80-84	0.003605
85-89	0.021134999999999998
90-94	0.06272
95-99	0.00259
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.08898576227803714
#Duplication Level	Relative count
1	100.0
2	0.06855004153031713
3	0.0010007305332893012
4	5.003652666446506E-4
5	5.003652666446506E-4
6	5.003652666446506E-4
7	0.0
8	0.0
9	0.0
10++	0.0020014610665786024
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	344050	3.5256224	4.362697	95-97
AAAAA	350955	3.487739	4.2086377	95-97
>>END_MODULE