##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R1_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70882575	34.0	31.0	34.0	31.0	34.0
2	32.9554475	34.0	31.0	34.0	31.0	34.0
3	32.99613275	34.0	31.0	34.0	31.0	34.0
4	36.41166125	37.0	37.0	37.0	35.0	37.0
5	36.339654	37.0	37.0	37.0	35.0	37.0
6	36.30650875	37.0	37.0	37.0	35.0	37.0
7	36.30183775	37.0	37.0	37.0	35.0	37.0
8	36.2890175	37.0	37.0	37.0	35.0	37.0
9	38.078969	39.0	38.0	39.0	37.0	39.0
10-14	38.35367505	39.4	38.4	39.4	35.8	39.4
15-19	39.5014682	41.0	39.8	41.0	37.0	41.0
20-24	39.269331900000005	40.8	39.0	41.0	35.8	41.0
25-29	38.61172775000001	40.0	38.4	41.0	34.4	41.0
30-34	37.44796255	39.8	36.6	41.0	32.0	41.0
35-39	37.0306371	38.6	35.0	40.6	32.6	41.0
40-44	36.1290603	36.8	35.0	40.0	31.8	41.0
45-49	35.51067975	35.0	35.0	39.0	31.8	41.0
50-54	34.5069145	35.0	34.6	36.6	30.4	40.4
55-59	33.980300750000005	35.0	34.0	35.0	30.0	39.0
60-64	33.47289695	35.0	34.0	35.0	29.6	37.0
65-69	33.27970535	35.0	33.4	35.0	29.6	35.8
70-74	32.70986094999999	35.0	33.0	35.0	27.8	35.0
75-79	32.387679500000004	34.8	32.6	35.0	28.2	35.0
80-84	32.4265819	35.0	33.0	35.0	27.4	35.0
85-89	32.03212595	35.0	33.0	35.0	25.8	35.0
90-94	31.794838650000003	35.0	33.0	35.0	26.2	35.0
95-99	31.370230600000003	35.0	33.0	35.0	24.4	35.0
100-101	30.157079375000002	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	10.0
4	22.0
5	109.0
6	330.0
7	867.0
8	2115.0
9	3582.0
10	4565.0
11	5078.0
12	5276.0
13	5259.0
14	5511.0
15	5843.0
16	6284.0
17	7036.0
18	7704.0
19	8388.0
20	8982.0
21	9954.0
22	11762.0
23	14550.0
24	18443.0
25	23355.0
26	29659.0
27	37316.0
28	47491.0
29	59162.0
30	74953.0
31	96233.0
32	128273.0
33	184065.0
34	308288.0
35	699444.0
36	1330000.0
37	654725.0
38	170337.0
39	24933.0
40	64.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.437955529176993	22.61634783634813	19.38762678189676	27.558069852578114
2	30.50453428854145	22.88959456155377	22.835544102124867	23.770327047779908
3	28.628874999999997	21.839875	22.010775	27.520475
4	30.616597519709693	20.727736825723415	20.226553681497325	28.42911197306956
5	30.851774999999996	19.730249999999998	19.532975	29.885
6	30.011074999999998	19.221474999999998	18.6756	32.09185
7	31.873849999999997	19.098825	19.235374999999998	29.79195
8	29.697574999999997	19.303224999999998	19.77555	31.223649999999996
9	30.259725	18.448725	19.7963	31.495250000000002
10-14	30.562060000000002	19.290355	19.451635	30.69595
15-19	30.601329999999997	19.206300000000002	19.364795	30.827575000000003
20-24	30.621808586064752	19.217404452334318	19.382866470970946	30.777920490629985
25-29	30.930400477642557	19.22127283380966	19.24990298698098	30.5984237015668
30-34	31.248951294541527	19.167894429566765	19.11036758846021	30.47278668743149
35-39	30.682946196644522	19.24497155893238	19.299617736808063	30.772464507615034
40-44	30.793502215721936	19.262067715462553	19.22319601482565	30.72123405398986
45-49	30.834905000000003	19.18105	19.193305	30.79074
50-54	30.866879080509797	19.248351196042872	19.233061159346782	30.651708564100556
55-59	30.7844	19.242945	19.221095	30.75156
60-64	30.832916898137626	19.17975243321422	19.17724207034127	30.810088598306884
65-69	30.736125390545304	19.255548643642662	19.26539978637522	30.742926179436814
70-74	30.88014	19.34298	19.195865	30.581015
75-79	30.794864999999998	19.21997	19.19159	30.793575
80-84	30.762621062301715	19.284452089661443	19.271791914318012	30.681134933718834
85-89	30.83232408895485	19.33539382532455	19.2402264974403	30.5920555882803
90-94	30.824665177103533	19.170371446115954	19.207067928549577	30.79789544823093
95-99	30.786962476143525	19.226867655242724	19.239612776321373	30.746557092292377
100-101	31.008775	19.232525	19.148075000000002	30.610625000000002
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.99602538175511
2	54.27486133632136
3	56.14935
4	59.04570949277925
5	60.736775
6	62.102925
7	61.665800000000004
8	60.921225
9	61.754975
10-14	61.25801
15-19	61.428905
20-24	61.39972907669473
25-29	61.528824179209366
30-34	61.72173798197302
35-39	61.45541070425956
40-44	61.514736269711804
45-49	61.625645
50-54	61.51858764461034
55-59	61.53596
60-64	61.643005496444516
65-69	61.47905156998211
70-74	61.461155
75-79	61.58844
80-84	61.44375599602054
85-89	61.42437967723515
90-94	61.622560625334465
95-99	61.5335195684359
100-101	61.6194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	21.5
2	13.0
3	13.5
4	14.5
5	16.0
6	20.0
7	29.0
8	40.5
9	54.5
10	70.5
11	89.5
12	111.0
13	140.0
14	182.5
15	235.0
16	315.0
17	416.0
18	502.0
19	622.5
20	838.0
21	1078.5
22	1378.0
23	1778.0
24	2247.5
25	2791.0
26	3498.0
27	4377.5
28	5341.5
29	6452.5
30	7742.5
31	9127.5
32	10543.0
33	12043.5
34	13579.0
35	14974.5
36	16557.5
37	18121.0
38	19256.0
39	20382.5
40	21432.0
41	22464.5
42	23480.5
43	24486.5
44	25812.0
45	27528.5
46	29851.5
47	32862.0
48	36556.0
49	40986.0
50	46169.0
51	52510.0
52	60042.0
53	68264.0
54	77251.5
55	86950.0
56	97006.5
57	107965.5
58	119272.0
59	130962.5
60	143232.0
61	155495.0
62	167701.5
63	178989.0
64	188160.0
65	195046.5
66	199090.5
67	198874.5
68	194041.0
69	184996.5
70	171619.0
71	154661.5
72	135137.5
73	113822.5
74	92268.5
75	71532.5
76	52841.5
77	37156.0
78	24513.0
79	14908.5
80	8454.0
81	4541.0
82	2338.5
83	1222.0
84	733.5
85	471.5
86	341.5
87	258.0
88	192.0
89	143.0
90	107.0
91	80.5
92	54.0
93	39.0
94	26.0
95	14.5
96	8.5
97	3.0
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002975
2	8.5E-4
3	0.0
4	0.001625
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00122
25-29	5.35E-4
30-34	0.055349999999999996
35-39	0.020455
40-44	0.0043749999999999995
45-49	0.0
50-54	2.3999999999999998E-4
55-59	0.0
60-64	0.014454999999999999
65-69	0.0116
70-74	0.0
75-79	0.0
80-84	0.001385
85-89	0.0077
90-94	0.017665
95-99	9.500000000000001E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.27141586108305493
#Duplication Level	Relative count
1	100.0
2	0.22804845653797381
3	0.00701687558578381
4	0.0015036161969536736
5	0.0015036161969536736
6	5.012053989845579E-4
7	5.012053989845579E-4
8	0.0010024107979691157
9	0.0
10++	0.0010024107979691157
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	390435	3.714521	4.3191104	70-74
TTTTT	382020	3.6529472	4.151645	95-97
>>END_MODULE