##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L003_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84523125	34.0	31.0	34.0	31.0	34.0
2	33.0769465	34.0	31.0	34.0	31.0	34.0
3	33.109792	34.0	33.0	34.0	31.0	34.0
4	36.48528225	37.0	37.0	37.0	35.0	37.0
5	36.42905525	37.0	37.0	37.0	35.0	37.0
6	36.39468625	37.0	37.0	37.0	35.0	37.0
7	36.3886265	37.0	37.0	37.0	35.0	37.0
8	36.374179	37.0	37.0	37.0	35.0	37.0
9	38.166868	39.0	39.0	39.0	37.0	39.0
10-14	38.43486779999999	39.4	39.0	39.4	37.0	39.4
15-19	39.586718950000005	41.0	40.0	41.0	37.0	41.0
20-24	39.359928149999995	40.8	39.0	41.0	36.4	41.0
25-29	38.924875	40.0	38.4	41.0	35.2	41.0
30-34	38.184585750000004	40.0	37.2	41.0	34.0	41.0
35-39	37.178655549999995	38.6	35.2	40.6	32.8	41.0
40-44	36.21497255	37.0	35.0	40.0	32.0	41.0
45-49	35.656035100000004	35.2	35.0	39.2	32.6	41.0
50-54	34.8223234	35.0	35.0	37.0	31.4	40.4
55-59	34.183135549999996	35.0	34.4	35.2	31.0	39.2
60-64	33.66076865	35.0	34.0	35.0	30.2	37.2
65-69	33.25144725	35.0	33.2	35.0	29.4	35.8
70-74	32.9455648	35.0	33.0	35.0	29.0	35.0
75-79	32.274677600000004	34.4	32.6	35.0	27.4	35.0
80-84	32.4780304	35.0	33.0	35.0	28.2	35.0
85-89	32.15815164999999	35.0	33.0	35.0	27.0	35.0
90-94	31.678233499999997	35.0	33.0	35.0	25.6	35.0
95-99	31.133729200000005	35.0	33.0	35.0	23.8	35.0
100-101	29.7981625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20.0
3	12.0
4	39.0
5	99.0
6	260.0
7	875.0
8	1961.0
9	3096.0
10	3988.0
11	4589.0
12	4811.0
13	4964.0
14	5298.0
15	5824.0
16	6364.0
17	7081.0
18	7713.0
19	8376.0
20	8955.0
21	10108.0
22	12009.0
23	14542.0
24	18412.0
25	22904.0
26	28764.0
27	35695.0
28	44308.0
29	55310.0
30	69246.0
31	88441.0
32	118393.0
33	166604.0
34	277060.0
35	683869.0
36	1376273.0
37	688056.0
38	187721.0
39	27885.0
40	75.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.420534462025394	22.63665932483481	19.355010487735974	27.587795725403826
2	30.44635	22.9568	22.77375	23.8231
3	28.692099999999996	21.7611	21.98915	27.55765
4	30.673275	20.62995	20.204825	28.49195
5	30.877850000000002	19.7229	19.51675	29.8825
6	30.053	19.247875	18.6479	32.051225
7	31.932775000000003	19.0379	19.2178	29.811525
8	29.706149999999997	19.245725	19.782625	31.265500000000003
9	30.304575	18.3961	19.76525	31.534075
10-14	30.57029	19.27599	19.446884999999998	30.706834999999998
15-19	30.626379999999997	19.193965	19.355645	30.82401
20-24	30.61550260184059	19.201579544640897	19.367902139273372	30.815015714245142
25-29	30.727915754770514	19.25231673831086	19.284966749738363	30.734800757180263
30-34	30.746652215263552	19.16276059478494	19.213042074539928	30.877545115411575
35-39	30.708457826074476	19.19185797722084	19.275176484930014	30.824507711774668
40-44	30.796042318416927	19.202217680887074	19.224367689747076	30.777372310948927
45-49	30.802574999999997	19.12689	19.18771	30.882825000000004
50-54	30.743094999999997	19.180844999999998	19.243299999999998	30.832759999999997
55-59	30.788919999999997	19.218165000000003	19.21957	30.773345000000003
60-64	30.81512253209977	19.10864306566104	19.185730436734552	30.89050396550464
65-69	30.757057699021562	19.177604628806197	19.251725348696	30.813612323476246
70-74	30.833515	19.2145	19.188785	30.7632
75-79	30.835655	19.14838	19.152145	30.863819999999997
80-84	30.767429887624985	19.206683497050218	19.22714477383389	30.798741841490905
85-89	30.858812106150175	19.212703043545904	19.191062407173128	30.737422443130797
90-94	30.87570709224041	19.11559111908304	19.168492191376522	30.840209597300028
95-99	30.827143393931383	19.166940787910967	19.214272333863775	30.79164348429388
100-101	31.126308951162784	19.155891113063657	19.037875972474886	30.679923963298677
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.008330187429216
2	54.26945
3	56.24975
4	59.165225
5	60.760349999999995
6	62.104225
7	61.744299999999996
8	60.97165
9	61.838649999999994
10-14	61.277125
15-19	61.45039
20-24	61.43051831608574
25-29	61.462716511950774
30-34	61.62419733067512
35-39	61.53296553784914
40-44	61.57341462936585
45-49	61.6854
50-54	61.575855000000004
55-59	61.562265000000004
60-64	61.705626497604406
65-69	61.57067002249781
70-74	61.596715
75-79	61.69947500000001
80-84	61.566171729115894
85-89	61.59623454928097
90-94	61.71591668954044
95-99	61.618786878225265
100-101	61.806232914461454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	16.5
2	9.5
3	8.0
4	8.5
5	15.0
6	21.5
7	21.5
8	29.5
9	39.0
10	50.5
11	70.5
12	94.5
13	119.5
14	154.5
15	210.5
16	278.5
17	364.0
18	479.0
19	591.5
20	793.0
21	1060.0
22	1331.5
23	1711.0
24	2180.0
25	2831.0
26	3608.5
27	4339.0
28	5265.0
29	6424.5
30	7764.5
31	9216.0
32	10568.5
33	12108.0
34	13658.5
35	15061.5
36	16430.5
37	17782.5
38	19136.5
39	20146.0
40	21256.5
41	22295.5
42	23128.5
43	24359.5
44	25736.0
45	27586.0
46	29987.0
47	32774.5
48	36419.5
49	40861.5
50	46069.0
51	52184.0
52	59650.0
53	67914.5
54	76846.5
55	86925.0
56	97055.0
57	107716.0
58	118863.5
59	130492.5
60	142847.0
61	154820.5
62	167066.5
63	178503.0
64	187472.0
65	194296.5
66	198078.0
67	197742.5
68	193492.5
69	184522.0
70	171213.0
71	155304.5
72	136188.0
73	115037.0
74	93309.5
75	72482.5
76	54372.5
77	38553.0
78	25430.5
79	15830.0
80	9242.5
81	5078.5
82	2767.0
83	1472.5
84	850.0
85	559.0
86	401.0
87	290.0
88	211.5
89	152.5
90	100.5
91	63.5
92	41.0
93	29.0
94	20.0
95	13.5
96	8.0
97	4.0
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0022500000000000003
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00156
25-29	3.5E-5
30-34	0.032775000000000006
35-39	0.00421
40-44	3.9999999999999996E-5
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.05496500000000001
65-69	0.034699999999999995
70-74	0.0
75-79	0.0
80-84	0.00624
85-89	0.04915
90-94	0.049284999999999995
95-99	0.01383
100-101	0.0022375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2949247941774782
#Duplication Level	Relative count
1	100.0
2	0.25518773093486946
3	0.008522969402539845
4	0.0030081068479552394
5	5.013511413258732E-4
6	0.0
7	0.0010027022826517465
8	0.0
9	0.0
10++	0.002005404565303493
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	358495	3.4672093	3.8153737	70-74
TTTTT	357665	3.4263484	3.6791308	90-94
>>END_MODULE