##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_009.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.430428	34.0	31.0	34.0	31.0	34.0
2	32.6051	34.0	31.0	34.0	31.0	34.0
3	32.63534175	34.0	31.0	34.0	31.0	34.0
4	36.0398575	37.0	37.0	37.0	35.0	37.0
5	36.04450825	37.0	37.0	37.0	35.0	37.0
6	36.0269365	37.0	37.0	37.0	35.0	37.0
7	36.002883	37.0	37.0	37.0	35.0	37.0
8	36.01258225	37.0	37.0	37.0	35.0	37.0
9	37.74311675	39.0	38.0	39.0	35.0	39.0
10-14	37.95469995	39.4	38.2	39.4	35.2	39.4
15-19	39.0890177	41.0	39.0	41.0	36.0	41.0
20-24	38.8841881	40.8	39.0	41.0	35.2	41.0
25-29	38.47003635	40.0	38.0	41.0	34.4	41.0
30-34	37.8168452	40.0	37.0	41.0	33.2	41.0
35-39	36.807234199999996	38.6	35.0	40.6	31.8	41.0
40-44	35.870027449999995	36.6	35.0	40.0	31.0	41.0
45-49	34.948100700000005	35.0	34.8	38.8	30.2	41.0
50-54	33.70695744999999	34.8	33.4	36.2	28.4	39.8
55-59	33.86720820000001	35.0	34.4	35.0	30.0	39.2
60-64	33.5055292	35.0	34.0	35.0	29.8	37.2
65-69	33.19179715	35.0	34.0	35.0	29.0	35.8
70-74	32.879731	35.0	33.4	35.0	29.0	35.0
75-79	32.59813355	35.0	33.0	35.0	27.6	35.0
80-84	32.31496815	35.0	33.0	35.0	27.0	35.0
85-89	31.9893168	35.0	33.0	35.0	26.6	35.0
90-94	31.585931799999997	35.0	33.0	35.0	24.4	35.0
95-99	31.055652200000004	35.0	32.8	35.0	23.0	35.0
100-101	29.95955275	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8976.0
3	1758.0
4	2099.0
5	2250.0
6	3215.0
7	3950.0
8	4786.0
9	5471.0
10	5895.0
11	5962.0
12	5993.0
13	5807.0
14	5791.0
15	5890.0
16	6310.0
17	7082.0
18	7721.0
19	8890.0
20	10448.0
21	12447.0
22	15078.0
23	18124.0
24	22217.0
25	26981.0
26	32564.0
27	39480.0
28	47790.0
29	58264.0
30	72131.0
31	91133.0
32	118738.0
33	163767.0
34	265888.0
35	636825.0
36	1341962.0
37	701130.0
38	190414.0
39	36566.0
40	207.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.643944102913395	23.042202465477043	19.00592994179117	27.307923489818393
2	30.756090496958944	23.58697919454325	22.087858823223396	23.56907148527441
3	29.370418965364593	21.76797111156353	20.96184907602723	27.899760847044654
4	31.23881060655704	20.526556160437956	19.393548006280817	28.841085226724182
5	30.764332739439205	19.81598952398769	19.41373546068144	30.005942275891666
6	30.213755894974483	19.071794580662456	18.32659627918539	32.387853245177666
7	32.07900377927021	18.948346972569716	19.137548078714666	29.83510116944541
8	29.632721849224392	19.343000790127878	19.79646766065871	31.227809699989017
9	30.435393820539502	18.286565476666933	19.513327463379493	31.76471323941407
10-14	30.60795476597357	19.284173692387654	19.368793435200452	30.73907810643832
15-19	30.679716379849424	19.148986503940865	19.29812970326855	30.87316741294116
20-24	30.643141760961495	19.16368633176802	19.30119870645656	30.891973200813922
25-29	30.747480740249706	19.26173057512613	19.23194132122067	30.758847363403497
30-34	30.81727294865767	19.129868611879914	19.14102697668482	30.9118314627776
35-39	30.743835734545755	19.186349366954907	19.2148804554892	30.85493444301014
40-44	30.79880047819261	19.220435833723858	19.172527566380026	30.8082361217035
45-49	30.864812776005024	19.099041201565093	19.10687196467296	30.929274057756928
50-54	30.75192791290236	19.164692676348814	19.195232038896755	30.88814737185207
55-59	30.80595799837178	19.235702584146274	19.17143397215227	30.786905445329676
60-64	30.840435	19.153755	19.120635	30.885174999999997
65-69	30.745540668547843	19.216626670663352	19.200816603470564	30.837016057318245
70-74	30.79461	19.27469	19.178955000000002	30.751745000000003
75-79	30.838640334381317	19.18147498545227	19.12250404781436	30.857380632352054
80-84	30.743023337095792	19.24094111609206	19.2027048122341	30.81333073457805
85-89	30.794025	19.293355000000002	19.199155	30.713465
90-94	30.861905	19.186505	19.131515	30.820075000000003
95-99	30.75474338692844	19.252164148645363	19.228714027877245	30.76437843654895
100-101	30.879894839421617	19.321218766703215	19.169505432279877	30.629380961595288
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.95186759273179
2	54.32516198223335
3	57.27017981240924
4	60.07989583328123
5	60.770275015330874
6	62.60160914015215
7	61.91410494871562
8	60.86053154921341
9	62.20010705995357
10-14	61.34703287241189
15-19	61.55288379279058
20-24	61.53511496177542
25-29	61.506328103653196
30-34	61.729104411435266
35-39	61.59877017755589
40-44	61.60703659989611
45-49	61.79408683376195
50-54	61.640075284754424
55-59	61.592863443701454
60-64	61.725609999999996
65-69	61.58255672586609
70-74	61.546355
75-79	61.696020966733364
80-84	61.556354071673844
85-89	61.50749
90-94	61.68198
95-99	61.5191218234774
100-101	61.509275801016905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	89.5
2	45.5
3	36.5
4	30.5
5	36.5
6	45.0
7	59.0
8	72.0
9	72.5
10	75.5
11	102.0
12	144.0
13	178.5
14	216.0
15	274.0
16	352.5
17	442.0
18	550.0
19	690.5
20	895.5
21	1150.0
22	1438.0
23	1812.0
24	2287.0
25	2851.0
26	3627.0
27	4474.5
28	5410.5
29	6580.0
30	7912.0
31	9304.0
32	10663.5
33	12097.5
34	13665.5
35	15102.5
36	16459.0
37	17944.0
38	19151.0
39	20171.5
40	21205.5
41	22201.5
42	23144.0
43	24227.5
44	25647.5
45	27323.0
46	29439.0
47	32184.0
48	35576.0
49	39754.0
50	44950.0
51	51278.5
52	58660.0
53	66873.5
54	75349.5
55	84541.0
56	94937.5
57	106079.5
58	117430.0
59	129248.5
60	141673.5
61	154233.0
62	166564.5
63	177802.0
64	187405.5
65	194886.5
66	199324.5
67	199780.5
68	195794.0
69	186893.5
70	173899.5
71	157240.5
72	137590.0
73	116671.0
74	95077.5
75	73798.0
76	54706.5
77	38625.5
78	25482.5
79	15683.5
80	8984.5
81	4784.5
82	2431.0
83	1215.5
84	667.5
85	442.0
86	336.5
87	263.0
88	221.5
89	176.5
90	133.5
91	115.0
92	98.5
93	82.5
94	68.5
95	58.0
96	48.0
97	39.0
98	32.5
99	25.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053825
2	0.04305
3	0.04305
4	0.04485
5	0.0383
6	0.036675
7	0.040225
8	0.047825
9	0.055999999999999994
10-14	0.029240000000000002
15-19	0.028965
20-24	0.012635
25-29	0.014280000000000001
30-34	0.030154999999999998
35-39	0.02134
40-44	0.0068200000000000005
45-49	0.009745
50-54	0.014285
55-59	0.0134
60-64	0.0
65-69	4.25E-4
70-74	0.0
75-79	0.00159
80-84	0.0034100000000000003
85-89	0.0
90-94	0.0
95-99	5.149999999999999E-4
100-101	5.5E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.4021702204192639
#Duplication Level	Relative count
1	100.0
2	0.29795494560065006
3	0.009028937745474246
4	0.004012861220210775
5	0.0025080382626317347
6	0.0010032153050526938
7	0.0015048229575790409
8	0.0010032153050526938
9	0.0
10++	0.004514468872737123
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	401290	3.845987	4.3759637	60-64
TTTTT	371770	3.6327744	3.985442	10-14
>>END_MODULE