##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_008.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.562424	34.0	31.0	34.0	31.0	34.0
2	32.7531315	34.0	31.0	34.0	31.0	34.0
3	32.7841845	34.0	31.0	34.0	31.0	34.0
4	36.13266825	37.0	37.0	37.0	35.0	37.0
5	36.15263725	37.0	37.0	37.0	35.0	37.0
6	36.15042225	37.0	37.0	37.0	35.0	37.0
7	36.1333075	37.0	37.0	37.0	35.0	37.0
8	36.10579625	37.0	37.0	37.0	35.0	37.0
9	37.8514035	39.0	39.0	39.0	35.0	39.0
10-14	38.108476350000004	39.4	38.6	39.4	35.2	39.4
15-19	39.24555175	41.0	39.6	41.0	36.2	41.0
20-24	39.036915449999995	41.0	39.0	41.0	35.6	41.0
25-29	38.58065620000001	40.2	38.2	41.0	35.0	41.0
30-34	37.961478850000006	40.0	37.2	41.0	33.4	41.0
35-39	36.9536186	38.6	35.2	40.8	32.4	41.0
40-44	35.99272135	37.0	35.0	40.0	31.2	41.0
45-49	35.07298264999999	35.0	35.0	38.8	30.8	41.0
50-54	33.77926795	34.8	33.4	36.2	28.6	39.8
55-59	33.971729800000006	35.0	35.0	35.2	30.4	39.2
60-64	33.5999861	35.0	34.2	35.0	30.0	37.2
65-69	33.2736466	35.0	34.0	35.0	29.6	36.0
70-74	32.960374200000004	35.0	33.8	35.0	29.0	35.0
75-79	32.6374383	35.0	33.0	35.0	28.2	35.0
80-84	32.31064465000001	35.0	33.0	35.0	27.0	35.0
85-89	31.966811199999995	35.0	33.0	35.0	26.4	35.0
90-94	31.546245649999996	35.0	33.0	35.0	24.4	35.0
95-99	30.9592085	35.0	32.6	35.0	22.6	35.0
100-101	29.826942250000002	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9730.0
3	2010.0
4	2291.0
5	2655.0
6	3190.0
7	3742.0
8	4434.0
9	4859.0
10	5289.0
11	5535.0
12	5583.0
13	5514.0
14	5467.0
15	5741.0
16	6031.0
17	6546.0
18	7476.0
19	8386.0
20	9912.0
21	11714.0
22	13979.0
23	16817.0
24	20513.0
25	25078.0
26	29870.0
27	36326.0
28	44221.0
29	54687.0
30	68130.0
31	86755.0
32	114975.0
33	163557.0
34	271935.0
35	655822.0
36	1340643.0
37	707402.0
38	196002.0
39	36952.0
40	231.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.61488929049927	23.002081511781135	19.027715286824716	27.35531391089488
2	30.758773615498253	23.597707810379408	22.05829660145006	23.58522197267228
3	29.375704604693308	21.716235643327543	20.972363233508183	27.935696518470966
4	31.216087512449224	20.492805405333733	19.41113324028573	28.879973841931317
5	30.74008275895192	19.82022366229625	19.449079130305577	29.990614448446255
6	30.17778032362307	19.017339290944843	18.31185109606561	32.493029289366476
7	32.10330016810709	18.940407425134296	19.131457542764227	29.824834863994383
8	29.65499809806002	19.26625157660814	19.789510300506517	31.28924002482532
9	30.427048267263235	18.240266924875574	19.53872770714545	31.793957100715737
10-14	30.597732893928413	19.276959865965427	19.38132670497436	30.743980535131797
15-19	30.680116023768072	19.142105702617528	19.286547579495437	30.89123069411896
20-24	30.644122578950896	19.1492864651404	19.312449735085362	30.894141220823347
25-29	30.79584522423497	19.244688161005637	19.21958141858996	30.739885196169432
30-34	30.82116938669937	19.123650570182107	19.13343545430702	30.921744588811507
35-39	30.71996129851834	19.183557592287272	19.217604452511033	30.878876656683357
40-44	30.820584671841942	19.199158159280042	19.18008542424984	30.80017174462818
45-49	30.85515322075516	19.087954251936903	19.123225468488823	30.933667058819115
50-54	30.76442674933218	19.15728411546531	19.196649994225904	30.881639140976606
55-59	30.77389034067915	19.217477603165072	19.189806011792008	30.818826044363767
60-64	30.83553	19.132905	19.10258	30.928984999999997
65-69	30.7417767652164	19.199485397041034	19.19425536696836	30.864482470774206
70-74	30.795155	19.243525	19.18342	30.777900000000002
75-79	30.8395724772812	19.13862437407513	19.125753991181238	30.896049157462436
80-84	30.746792348389256	19.22516827998269	19.202216747967928	30.82582262366013
85-89	30.80355	19.263145	19.19564	30.737664999999996
90-94	30.859165	19.160635	19.125505	30.854695
95-99	30.782462562404326	19.20691473310861	19.205524706350594	30.805097998136468
100-101	30.916490850166838	19.259775659896263	19.18268640611574	30.641047083821153
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.97020320139414
2	54.343995588170536
3	57.31140112316427
4	60.09606135438054
5	60.73069720739818
6	62.670809612989544
7	61.92813503210147
8	60.94423812288534
9	62.221005367978975
10-14	61.34171342906022
15-19	61.57134671788703
20-24	61.53826379977424
25-29	61.5357304204044
30-34	61.742913975510874
35-39	61.59883795520169
40-44	61.62075641647012
45-49	61.788820279574274
50-54	61.64606589030879
55-59	61.59271638504292
60-64	61.764514999999996
65-69	61.60625923599061
70-74	61.573055
75-79	61.73562163474363
80-84	61.57261497204939
85-89	61.54121500000001
90-94	61.71386
95-99	61.58756056054079
100-101	61.557537933987994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	118.5
2	56.5
3	49.5
4	52.0
5	52.5
6	49.5
7	53.0
8	69.5
9	82.0
10	99.0
11	121.5
12	148.0
13	185.0
14	220.0
15	271.0
16	347.0
17	436.5
18	546.5
19	713.5
20	933.0
21	1165.0
22	1451.5
23	1836.0
24	2333.0
25	2941.0
26	3621.5
27	4460.5
28	5445.0
29	6558.5
30	7774.0
31	9069.0
32	10462.5
33	12041.0
34	13628.5
35	15004.5
36	16373.0
37	17775.0
38	19020.5
39	20122.5
40	21268.5
41	22331.5
42	23164.5
43	24127.5
44	25532.0
45	27421.0
46	29476.0
47	32035.5
48	35678.0
49	39800.0
50	44987.0
51	51479.0
52	58640.5
53	66772.0
54	75487.0
55	84924.0
56	95175.5
57	106097.5
58	117938.5
59	129593.0
60	141475.0
61	154223.0
62	166500.5
63	177683.5
64	187252.5
65	194335.0
66	198989.0
67	199327.5
68	194729.0
69	186499.5
70	173715.0
71	157057.0
72	137557.5
73	116051.0
74	94332.0
75	73688.0
76	54922.0
77	38750.0
78	25838.5
79	16107.5
80	9315.0
81	5155.5
82	2788.0
83	1550.5
84	962.5
85	657.0
86	515.5
87	430.0
88	341.0
89	281.0
90	245.5
91	201.5
92	174.5
93	147.5
94	123.0
95	107.5
96	85.5
97	66.5
98	48.5
99	32.5
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09665
2	0.0868
3	0.07695
4	0.069075
5	0.05915
6	0.055025
7	0.078575
8	0.10200000000000001
9	0.106725
10-14	0.04967
15-19	0.056685000000000006
20-24	0.023455
25-29	0.026855
30-34	0.049914999999999994
35-39	0.034835
40-44	0.014339999999999999
45-49	0.017625000000000002
50-54	0.027635000000000003
55-59	0.023819999999999997
60-64	0.0
65-69	5.75E-4
70-74	0.0
75-79	0.002975
80-84	0.0066749999999999995
85-89	0.0
90-94	0.0
95-99	0.001925
100-101	0.002275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.34484999025423313
#Duplication Level	Relative count
1	100.0
2	0.29087699413732404
3	0.006519656765146918
4	0.0010030241177149104
5	5.015120588574552E-4
6	0.0
7	0.0
8	0.0
9	5.015120588574552E-4
10++	0.0030090723531447316
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	401490	3.87344	4.370557	60-64
TTTTT	377820	3.6941142	4.1184144	10-14
>>END_MODULE