##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_007.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62906775	34.0	31.0	34.0	31.0	34.0
2	32.80490625	34.0	31.0	34.0	31.0	34.0
3	32.848328	34.0	33.0	34.0	31.0	34.0
4	36.18939725	37.0	37.0	37.0	35.0	37.0
5	36.2018125	37.0	37.0	37.0	35.0	37.0
6	36.1988285	37.0	37.0	37.0	35.0	37.0
7	36.18333375	37.0	37.0	37.0	35.0	37.0
8	36.14283225	37.0	37.0	37.0	35.0	37.0
9	37.9043145	39.0	39.0	39.0	37.0	39.0
10-14	38.16712595	39.4	39.2	39.4	36.6	39.4
15-19	39.13103115	41.0	39.8	41.0	36.4	41.0
20-24	38.57146335	40.6	38.6	41.0	34.2	41.0
25-29	38.65761245	40.0	38.4	41.0	35.0	41.0
30-34	38.07263395	40.0	37.4	41.0	34.0	41.0
35-39	36.9257126	38.8	35.2	40.8	32.2	41.0
40-44	36.047920850000004	37.0	35.0	40.0	31.8	41.0
45-49	35.116931650000005	35.0	35.0	39.0	30.8	41.0
50-54	33.873476700000005	34.8	33.6	36.2	29.2	39.8
55-59	34.072526599999996	35.0	35.0	35.2	31.0	39.2
60-64	33.6911463	35.0	34.6	35.0	30.6	37.2
65-69	33.35679935	35.0	34.0	35.0	30.0	36.0
70-74	33.04590425	35.0	34.0	35.0	29.0	35.0
75-79	32.57715265	35.0	33.0	35.0	28.2	35.0
80-84	32.3360832	35.0	33.0	35.0	27.0	35.0
85-89	32.0000114	35.0	33.0	35.0	27.0	35.0
90-94	31.24014105	35.0	32.2	35.0	22.4	35.0
95-99	30.806458550000002	35.0	32.2	35.0	21.8	35.0
100-101	29.156022	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12593.0
3	2053.0
4	2304.0
5	2545.0
6	3137.0
7	3713.0
8	4255.0
9	4645.0
10	4915.0
11	5296.0
12	5157.0
13	5140.0
14	5259.0
15	5181.0
16	5709.0
17	6350.0
18	7117.0
19	8205.0
20	9560.0
21	11243.0
22	13534.0
23	16275.0
24	19669.0
25	23880.0
26	28176.0
27	34449.0
28	42359.0
29	52060.0
30	65567.0
31	84989.0
32	114523.0
33	167868.0
34	298926.0
35	725917.0
36	1316544.0
37	663218.0
38	184359.0
39	33131.0
40	179.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.598395866427218	22.985535650737944	19.038060794994514	27.378007687840324
2	30.75007299255451	23.593071641879245	22.059927513260106	23.596927852306138
3	29.332896409825572	21.73235497848719	21.02151981789402	27.913228793793216
4	31.238170469386766	20.46758464575108	19.40976618014476	28.8844787047174
5	30.7550559761865	19.779553957399887	19.420044012125985	30.04534605428763
6	30.198893741922326	19.072579888423324	18.307657853218746	32.420868516435604
7	32.098349072960616	18.913841783508957	19.112887416034667	29.874921727495764
8	29.58353632801101	19.31290319752624	19.79535631671428	31.30820415774847
9	30.42824635029412	18.260239026461228	19.548747697213113	31.762766926031542
10-14	30.587191696932592	19.27706706844177	19.373795585572132	30.76194564905351
15-19	30.91564315687318	19.15437067469426	19.191143391720995	30.738842776711568
20-24	30.763907073809204	19.275622509638744	19.182719733402205	30.77775068314985
25-29	30.735931515638367	19.22154193078158	19.255152917481674	30.78737363609838
30-34	30.820468532172306	19.110332058576205	19.134638804606542	30.934560604644943
35-39	30.82547474602838	19.19620144140017	19.176529019249205	30.801794793322244
40-44	30.798145078647153	19.184300066057084	19.17237337041015	30.845181484885615
45-49	30.865732498908923	19.11889287486605	19.077495418371388	30.93787920785364
50-54	30.74271706895123	19.151649959792223	19.193904148986118	30.911728822270423
55-59	30.801806691024296	19.209499635996302	19.18828776583376	30.800405907145638
60-64	30.85622968498375	19.114615291692232	19.099515279612223	30.929639743711796
65-69	30.734453073445305	19.208791920879193	19.20137692013769	30.85537808553781
70-74	30.812614999999997	19.247835	19.144935	30.794614999999997
75-79	30.916565774111753	19.175822670192055	19.089294761541208	30.81831679415499
80-84	30.745897023773207	19.20078353868635	19.21991192441062	30.833407513129824
85-89	30.833651541682578	19.245205962260297	19.173950958697546	30.747191537359576
90-94	30.94624342691022	19.213309735235402	19.077854173094817	30.76259266475956
95-99	30.77698311703742	19.205660070911403	19.202984791612245	30.814372020438935
100-101	31.064170414907892	19.428224259544965	19.123238520429055	30.38436680511809
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.97640355426754
2	54.34700084486065
3	57.24612520361879
4	60.12264917410416
5	60.80040203047413
6	62.61976225835792
7	61.97327080045638
8	60.891740485759485
9	62.191013276325656
10-14	61.34913734598611
15-19	61.654485933584745
20-24	61.541657756959054
25-29	61.52330515173674
30-34	61.75502913681725
35-39	61.62726953935063
40-44	61.64332656353276
45-49	61.80361170676256
50-54	61.654445891221656
55-59	61.602212598169935
60-64	61.78586942869554
65-69	61.58983115898311
70-74	61.60723
75-79	61.734882568266734
80-84	61.57930453690304
85-89	61.58084307904216
90-94	61.70883609166978
95-99	61.591355137476356
100-101	61.448537220025976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	81.5
2	52.0
3	48.0
4	51.0
5	51.0
6	52.0
7	60.5
8	81.0
9	106.0
10	111.0
11	132.0
12	173.5
13	220.5
14	281.0
15	334.5
16	419.5
17	517.0
18	669.5
19	858.5
20	1008.0
21	1211.5
22	1539.0
23	1947.0
24	2420.0
25	2952.5
26	3662.0
27	4556.0
28	5356.0
29	6448.5
30	7794.0
31	9131.0
32	10674.0
33	12151.5
34	13599.0
35	14997.5
36	16316.0
37	17734.0
38	19155.0
39	20304.0
40	21276.5
41	22144.5
42	23245.0
43	24443.5
44	25694.0
45	27271.5
46	29418.5
47	32207.0
48	35633.5
49	39834.0
50	44960.5
51	51135.0
52	58292.5
53	66568.5
54	75357.5
55	84667.5
56	95122.5
57	105928.0
58	117172.0
59	129341.5
60	141664.5
61	154130.0
62	166290.5
63	177534.0
64	187200.5
65	194300.5
66	198866.5
67	199229.5
68	195202.5
69	186840.5
70	173569.5
71	156925.5
72	137255.5
73	116089.0
74	94608.0
75	73496.5
76	54572.5
77	38702.5
78	25873.0
79	16166.5
80	9499.5
81	5342.0
82	2920.5
83	1675.5
84	1076.5
85	743.5
86	575.5
87	453.0
88	389.5
89	332.0
90	263.0
91	232.5
92	192.5
93	165.5
94	156.0
95	129.5
96	101.0
97	82.0
98	59.5
99	37.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.173525
2	0.16105
3	0.135075
4	0.11754999999999999
5	0.107075
6	0.091125
7	0.148525
8	0.192375
9	0.18887500000000002
10-14	0.09668
15-19	0.11616499999999999
20-24	0.06219
25-29	0.06244
30-34	0.089465
35-39	0.06314499999999999
40-44	0.056139999999999995
45-49	0.03009
50-54	0.06908
55-59	0.05596
60-64	7.999999999999999E-5
65-69	9.999999999999999E-6
70-74	0.0
75-79	0.00914
80-84	0.0177
85-89	4.9999999999999996E-6
90-94	4.15E-4
95-99	0.01044
100-101	0.028112500000000002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.321826213844528
#Duplication Level	Relative count
1	100.0
2	0.25819195443789356
3	0.004512092407652509
4	0.002005374403401115
5	0.0010026872017005575
6	5.013436008502787E-4
7	0.0
8	0.0
9	0.0
10++	0.0025067180042513937
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	406310	3.9052262	4.549765	1
TTTTT	387780	3.806967	4.168043	85-89
>>END_MODULE