##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_006.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36824575	34.0	31.0	34.0	31.0	34.0
2	32.5610525	34.0	31.0	34.0	31.0	34.0
3	32.595813	34.0	31.0	34.0	31.0	34.0
4	35.98931525	37.0	37.0	37.0	35.0	37.0
5	35.97970525	37.0	37.0	37.0	35.0	37.0
6	35.99343775	37.0	37.0	37.0	35.0	37.0
7	35.97907275	37.0	37.0	37.0	35.0	37.0
8	35.99022125	37.0	37.0	37.0	35.0	37.0
9	37.70746675	39.0	38.0	39.0	35.0	39.0
10-14	37.93570015	39.4	38.2	39.4	35.2	39.4
15-19	38.99755495	41.0	39.0	41.0	36.0	41.0
20-24	38.8325051	40.6	39.0	41.0	35.0	41.0
25-29	38.4615911	40.0	38.0	41.0	34.4	41.0
30-34	37.76287685	40.0	36.8	41.0	33.0	41.0
35-39	36.78635355	38.6	35.0	40.6	31.8	41.0
40-44	35.8394805	36.6	35.0	40.0	31.0	41.0
45-49	34.9563021	35.0	34.8	38.8	30.4	41.0
50-54	33.71218985	34.8	33.2	36.2	28.2	39.8
55-59	33.86053165	35.0	34.0	35.0	30.0	39.0
60-64	33.5090508	35.0	34.0	35.0	29.6	37.0
65-69	33.1945625	35.0	34.0	35.0	29.0	35.8
70-74	32.91033	35.0	33.4	35.0	29.0	35.0
75-79	32.59968765	35.0	33.0	35.0	27.4	35.0
80-84	32.28971325	35.0	33.0	35.0	27.0	35.0
85-89	32.00638965	35.0	33.0	35.0	26.8	35.0
90-94	31.597771599999998	35.0	33.0	35.0	24.4	35.0
95-99	31.035797649999996	35.0	32.6	35.0	23.0	35.0
100-101	29.978349625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9693.0
3	1778.0
4	1966.0
5	2368.0
6	2960.0
7	3822.0
8	4501.0
9	5287.0
10	5497.0
11	5564.0
12	5641.0
13	5704.0
14	5511.0
15	5576.0
16	5999.0
17	6689.0
18	7558.0
19	8795.0
20	10273.0
21	12072.0
22	14788.0
23	18095.0
24	22087.0
25	27012.0
26	32515.0
27	40284.0
28	48351.0
29	60018.0
30	74819.0
31	94896.0
32	123672.0
33	171148.0
34	274639.0
35	638986.0
36	1325139.0
37	693672.0
38	186822.0
39	35602.0
40	201.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.614785727199383	23.00342001134829	19.00953386382155	27.372260397630782
2	30.717592111675668	23.612583412201392	22.072234547137985	23.597589928984956
3	29.31018778026906	21.7786324871877	21.016275624599615	27.894904107943624
4	31.16665911605819	20.54067529471777	19.443475936377656	28.849189652846384
5	30.767382225217204	19.83316928788924	19.399458499840293	29.99998998705326
6	30.216514394500955	19.082791974429778	18.287660017025146	32.413033614044124
7	32.03461287774862	18.942302619917132	19.1537890930583	29.869295409275953
8	29.691509896286515	19.347590186724272	19.74984247952234	31.21105743746687
9	30.413618611462173	18.299809102180095	19.5478217293432	31.738750557014534
10-14	30.604703564962765	19.286734435142336	19.384963506737353	30.72359849315754
15-19	30.680047894098184	19.13635922953642	19.3098045850994	30.873788291265996
20-24	30.64294023938128	19.17700205355236	19.323312221809054	30.85674548525731
25-29	30.71629	19.27022	19.252675	30.760815
30-34	30.81417559192306	19.15409290400365	19.135547024928982	30.896184479144313
35-39	30.72943	19.204285	19.216815	30.84947
40-44	30.812885	19.209715	19.179655	30.797745
45-49	30.852065	19.12415	19.10087	30.922915
50-54	30.738255355709	19.17108593399722	19.20850643356089	30.882152276732892
55-59	30.78264423479327	19.242880772864233	19.194160758248227	30.78031423409427
60-64	30.82603	19.14636	19.133725	30.893884999999997
65-69	30.70523794573779	19.21667939419276	19.239044440041102	30.839038220028353
70-74	30.758760000000002	19.278064999999998	19.20249	30.760685
75-79	30.8209	19.175155	19.129794999999998	30.87415
80-84	30.725350000000002	19.234765	19.2323	30.807585
85-89	30.77092	19.280045	19.21978	30.729255
90-94	30.838905	19.203475	19.143165	30.814455000000002
95-99	30.72424	19.24486	19.24651	30.784390000000002
100-101	30.877412500000002	19.3578375	19.1619375	30.6028125
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.987046124830165
2	54.31518204066062
3	57.20509188821269
4	60.015848768904576
5	60.767372212270466
6	62.62954800854508
7	61.903908287024564
8	60.90256733375339
9	62.15236916847671
10-14	61.32830205812031
15-19	61.553836185364176
20-24	61.49968572463859
25-29	61.477105
30-34	61.71036007106737
35-39	61.578900000000004
40-44	61.61063
45-49	61.77498
50-54	61.620407632441896
55-59	61.562958468887544
60-64	61.719915
65-69	61.54427616576614
70-74	61.519445
75-79	61.695049999999995
80-84	61.532935
85-89	61.500175
90-94	61.65336
95-99	61.50863
100-101	61.480225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	177.0
1	114.0
2	52.5
3	54.0
4	62.5
5	62.5
6	65.0
7	84.5
8	94.5
9	99.0
10	107.5
11	133.5
12	160.5
13	201.0
14	251.0
15	286.5
16	332.5
17	413.5
18	553.5
19	724.5
20	890.0
21	1127.5
22	1467.0
23	1880.0
24	2346.0
25	2921.5
26	3623.5
27	4373.0
28	5345.0
29	6582.5
30	7858.5
31	9167.0
32	10596.0
33	12057.0
34	13558.5
35	15124.5
36	16452.5
37	17747.0
38	19117.5
39	20208.0
40	21277.5
41	22224.0
42	23127.5
43	24301.0
44	25643.5
45	27340.5
46	29293.0
47	32064.0
48	35524.5
49	39942.0
50	45405.5
51	51277.0
52	58401.0
53	66495.5
54	75319.0
55	85093.5
56	95350.5
57	106110.5
58	117388.0
59	129405.0
60	141881.0
61	154458.0
62	166641.5
63	177992.0
64	187460.5
65	195287.0
66	200014.0
67	200490.5
68	196151.0
69	186944.0
70	173986.5
71	156965.5
72	137452.0
73	116080.0
74	94169.5
75	73611.0
76	54790.0
77	38202.0
78	24895.0
79	15268.0
80	8801.5
81	4699.0
82	2404.0
83	1218.0
84	688.5
85	446.0
86	329.0
87	243.5
88	201.0
89	194.5
90	157.0
91	111.5
92	87.5
93	69.5
94	60.5
95	57.0
96	48.0
97	33.5
98	17.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16115000000000002
2	0.123275
3	0.096
4	0.118425
5	0.1293
6	8.749999999999999E-4
7	0.005425
8	0.013
9	0.025050000000000003
10-14	0.004144999999999999
15-19	2.05E-4
20-24	1.15E-4
25-29	0.0
30-34	0.00474
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0013349999999999998
55-59	2.9999999999999997E-5
60-64	0.0
65-69	2.05E-4
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3557918617608724
#Duplication Level	Relative count
1	100.0
2	0.28936084852435995
3	0.005516411323687972
4	0.0010029838770341767
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0025074596925854418
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	404880	3.8689888	4.3866973	60-64
TTTTT	382770	3.7205532	4.097841	95-97
>>END_MODULE