##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4845775	34.0	31.0	34.0	31.0	34.0
2	32.685024	34.0	31.0	34.0	31.0	34.0
3	32.6888815	34.0	31.0	34.0	31.0	34.0
4	36.087699	37.0	37.0	37.0	35.0	37.0
5	36.08092475	37.0	37.0	37.0	35.0	37.0
6	36.0825875	37.0	37.0	37.0	35.0	37.0
7	36.08226825	37.0	37.0	37.0	35.0	37.0
8	36.07464875	37.0	37.0	37.0	35.0	37.0
9	37.81221825	39.0	38.0	39.0	35.0	39.0
10-14	38.086278799999995	39.4	38.4	39.4	35.2	39.4
15-19	39.156650850000005	41.0	39.2	41.0	36.0	41.0
20-24	38.921115099999994	41.0	39.0	41.0	35.4	41.0
25-29	38.586863449999996	40.2	38.2	41.0	34.8	41.0
30-34	37.912538899999994	40.0	37.2	41.0	33.4	41.0
35-39	36.92262865	38.6	35.2	40.8	32.4	41.0
40-44	35.945530250000004	37.0	35.0	40.0	31.0	41.0
45-49	35.06168055	35.0	34.8	38.8	30.6	41.0
50-54	33.81205845	34.8	33.4	36.2	28.6	39.8
55-59	33.98740935	35.0	35.0	35.2	30.6	39.2
60-64	33.58460155	35.0	34.2	35.0	30.0	37.2
65-69	33.2331805	35.0	34.0	35.0	29.2	36.0
70-74	32.94499215	35.0	33.8	35.0	29.0	35.0
75-79	32.619537949999994	35.0	33.0	35.0	28.0	35.0
80-84	32.30265395	35.0	33.0	35.0	27.0	35.0
85-89	31.989835199999995	35.0	33.0	35.0	26.6	35.0
90-94	31.576597549999995	35.0	33.0	35.0	24.4	35.0
95-99	31.005723	35.0	32.8	35.0	23.0	35.0
100-101	29.772272875	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8795.0
3	1831.0
4	2236.0
5	2511.0
6	3241.0
7	3963.0
8	4559.0
9	5091.0
10	5536.0
11	5439.0
12	5571.0
13	5535.0
14	5642.0
15	5891.0
16	6251.0
17	6918.0
18	7611.0
19	8753.0
20	10114.0
21	11858.0
22	14246.0
23	17315.0
24	20990.0
25	25510.0
26	30669.0
27	37405.0
28	45211.0
29	55676.0
30	69568.0
31	88499.0
32	116373.0
33	164877.0
34	269974.0
35	648859.0
36	1341843.0
37	704778.0
38	193012.0
39	37619.0
40	230.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.59079560869365	23.00925007563963	19.03751825870557	27.362436056961148
2	30.737843240547303	23.60758359055301	22.082392753916384	23.572180414983304
3	29.31100000801064	21.718517190829424	21.07658971113639	27.893893090023553
4	31.224314183215125	20.460500142832856	19.431001965610452	28.884183708341567
5	30.719840076156117	19.831663328660376	19.445499525401356	30.00299706978215
6	30.17267775857635	19.057369462258855	18.336698864358425	32.43325391480637
7	32.06893877418274	18.914431960612134	19.137089490814752	29.87953977439038
8	29.63307402770397	19.326575974747975	19.787316341859093	31.25303365568896
9	30.452740906082383	18.279171666189132	19.535735689896562	31.73235173783192
10-14	30.580443694815756	19.280886669347723	19.387043495137085	30.751626140699432
15-19	30.727997723070725	19.15560772101475	19.275990704039152	30.84040385187537
20-24	30.659378938590542	19.158834574246676	19.309487381408484	30.8722991057543
25-29	30.73811346251577	19.240961969090645	19.24260713171497	30.778317436678616
30-34	30.810521283080327	19.104143128710817	19.143150968141164	30.942184620067696
35-39	30.73459133699773	19.200648595788415	19.209944682036095	30.85481538517776
40-44	30.80868615407001	19.214087012045102	19.164230553743696	30.812996280141192
45-49	30.86399313888919	19.089624760001627	19.105910497745548	30.940471603363633
50-54	30.732195054069038	19.16637627399627	19.208683848412175	30.892744823522513
55-59	30.783932810345107	19.243226328708936	19.18855802947708	30.78428283146888
60-64	30.83676	19.1384	19.109524999999998	30.915315
65-69	30.729231536461576	19.213035960651798	19.215715960785797	30.842016542100826
70-74	30.802030000000002	19.279665	19.16319	30.755115
75-79	30.83690900858162	19.155605973454335	19.148180955634295	30.859304062329752
80-84	30.740220457369915	19.22561167532551	19.22503166909044	30.809136198214134
85-89	30.818089999999998	19.277715	19.178925	30.72527
90-94	30.860349999999997	19.179445	19.122355	30.83785
95-99	30.773252317142948	19.21538515615512	19.207480137578322	30.803882389123615
100-101	30.91972333598837	19.362718259414763	19.14567761232538	30.57188079227149
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.953231665654805
2	54.31002365553061
3	57.20489309803419
4	60.10849789155669
5	60.72283714593827
6	62.60593167338272
7	61.948478548573114
8	60.88610768339293
9	62.18509264391431
10-14	61.33206983551519
15-19	61.568401574946094
20-24	61.53167804434484
25-29	61.51643089919438
30-34	61.75270590314802
35-39	61.58940672217549
40-44	61.6216824342112
45-49	61.80446474225283
50-54	61.62493987759156
55-59	61.568215641813985
60-64	61.752075
65-69	61.571248078562405
70-74	61.557145000000006
75-79	61.69621307091136
80-84	61.54935665558404
85-89	61.54336
90-94	61.6982
95-99	61.577134706266555
100-101	61.49160412825986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	64.5
2	41.5
3	36.5
4	32.0
5	40.0
6	55.5
7	61.5
8	63.5
9	68.0
10	79.5
11	103.0
12	126.0
13	162.0
14	223.0
15	290.5
16	352.5
17	441.0
18	542.5
19	702.5
20	912.0
21	1123.0
22	1417.5
23	1815.0
24	2294.0
25	2897.5
26	3620.0
27	4446.5
28	5426.5
29	6588.0
30	7852.5
31	9243.0
32	10585.0
33	11986.0
34	13606.5
35	15048.0
36	16411.0
37	17916.5
38	19084.0
39	20144.0
40	21356.0
41	22380.5
42	23222.0
43	24214.0
44	25744.5
45	27508.5
46	29600.5
47	32206.5
48	35515.5
49	39708.0
50	44814.5
51	51265.5
52	58716.0
53	66772.0
54	75466.5
55	84742.0
56	95143.5
57	106188.5
58	117719.5
59	129874.5
60	142078.0
61	154242.0
62	166569.5
63	178258.5
64	187829.0
65	194820.0
66	198862.0
67	199047.0
68	195014.5
69	186602.5
70	173547.5
71	156713.0
72	137230.5
73	116233.5
74	94499.0
75	73466.0
76	54732.5
77	38472.5
78	25413.5
79	15929.0
80	9159.0
81	4936.5
82	2679.0
83	1483.5
84	914.0
85	620.5
86	455.0
87	374.0
88	339.0
89	267.0
90	208.5
91	177.0
92	147.0
93	124.5
94	107.0
95	86.0
96	78.0
97	65.5
98	48.5
99	35.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1846
2	0.1502
3	0.1328
4	0.145525
5	0.152475
6	0.06530000000000001
7	0.07075000000000001
8	0.0793
9	0.094625
10-14	0.0394
15-19	0.023245000000000002
20-24	0.01182
25-29	0.009885
30-34	0.032915
35-39	0.011685
40-44	0.002925
45-49	0.00453
50-54	0.006085
55-59	0.006035
60-64	0.0
65-69	4.9999999999999996E-6
70-74	0.0
75-79	2.3999999999999998E-4
80-84	0.001075
85-89	0.0
90-94	0.0
95-99	2.35E-4
100-101	0.00145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.3353373613797361
#Duplication Level	Relative count
1	100.0
2	0.272782886885361
3	0.007521586954559586
4	0.0020057565212158895
5	0.0015043173909119172
6	0.0
7	5.014391303039724E-4
8	0.0
9	0.0015043173909119172
10++	0.0020057565212158895
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	394420	3.78382	4.2515125	60-64
TTTTT	377145	3.6912034	4.022679	95-97
>>END_MODULE