##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63892275	34.0	31.0	34.0	31.0	34.0
2	32.83699625	34.0	31.0	34.0	31.0	34.0
3	32.8510335	34.0	33.0	34.0	31.0	34.0
4	36.21467225	37.0	37.0	37.0	35.0	37.0
5	36.21807	37.0	37.0	37.0	35.0	37.0
6	36.1875265	37.0	37.0	37.0	35.0	37.0
7	36.1761015	37.0	37.0	37.0	35.0	37.0
8	36.161815	37.0	37.0	37.0	35.0	37.0
9	37.93518975	39.0	39.0	39.0	37.0	39.0
10-14	38.2353842	39.4	39.2	39.4	37.0	39.4
15-19	39.41160004999999	41.0	40.0	41.0	37.0	41.0
20-24	39.227202899999995	41.0	39.6	41.0	36.2	41.0
25-29	38.8365522	40.6	38.6	41.0	35.0	41.0
30-34	38.16118795	40.0	37.4	41.0	34.2	41.0
35-39	37.1990913	39.0	35.2	41.0	33.0	41.0
40-44	36.196939300000004	37.0	35.0	40.0	32.2	41.0
45-49	35.28680105	35.0	35.0	39.0	31.2	41.0
50-54	34.052186600000006	34.8	33.8	36.2	29.8	39.8
55-59	34.23193155	35.0	35.0	35.2	31.0	39.2
60-64	33.836291	35.0	35.0	35.0	31.0	37.2
65-69	33.495885400000006	35.0	34.4	35.0	30.4	36.0
70-74	33.1982289	35.0	34.0	35.0	29.8	35.0
75-79	32.917745350000004	35.0	33.8	35.0	29.0	35.0
80-84	32.61199755	35.0	33.0	35.0	28.6	35.0
85-89	32.278282749999995	35.0	33.0	35.0	27.0	35.0
90-94	31.9175495	35.0	33.0	35.0	26.6	35.0
95-99	31.383862799999996	35.0	33.0	35.0	24.2	35.0
100-101	30.333034875	34.0	31.5	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10365.0
3	1712.0
4	1926.0
5	2272.0
6	2612.0
7	3099.0
8	3759.0
9	3994.0
10	4323.0
11	4581.0
12	4578.0
13	4732.0
14	4625.0
15	4956.0
16	5278.0
17	5831.0
18	6546.0
19	7361.0
20	8409.0
21	10160.0
22	12046.0
23	14646.0
24	17806.0
25	21599.0
26	26048.0
27	31854.0
28	39228.0
29	48138.0
30	60754.0
31	77425.0
32	103064.0
33	148097.0
34	249520.0
35	634770.0
36	1403209.0
37	760042.0
38	208523.0
39	41804.0
40	308.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.630622958276575	22.965941260484456	19.00655706004852	27.396878721190443
2	30.772007740856548	23.511700159473662	22.07658741384792	23.639704685821865
3	29.36309497432446	21.735917135275564	20.987244474520867	27.9137434158791
4	31.227834404759104	20.46044353471865	19.380622606941877	28.931099453580366
5	30.76561196905735	19.79194807139232	19.38109195590795	30.061348003642372
6	30.230377752566657	18.98073353671492	18.298104021262862	32.49078468945556
7	32.10447758576237	18.889933168080535	19.10586593977485	29.899723306382242
8	29.670591537013973	19.285179124935166	19.759947278608998	31.284282059441864
9	30.397710975598454	18.228350825755733	19.555456911826116	31.8184812868197
10-14	30.59839486243806	19.21909479215414	19.383785020836044	30.79872532457176
15-19	30.68395430206244	19.10491018956843	19.28428376516213	30.926851743207003
20-24	30.669785704824342	19.124201948296275	19.298445603477788	30.9075667434016
25-29	30.73760043640258	19.222803035800524	19.24317683062625	30.79641969717064
30-34	30.79892950226802	19.094860352196015	19.136085719926147	30.970124425609814
35-39	30.736960759519583	19.146381301617016	19.20954380407132	30.907114134792085
40-44	30.824138065071327	19.175174068880754	19.154758204578567	30.845929661469352
45-49	30.865813058423853	19.063179121884723	19.097273282711793	30.97373453697963
50-54	30.757735867784703	19.145741826922645	19.186531043480514	30.909991261812138
55-59	30.809232611892917	19.199524190391095	19.164001596440112	30.827241601275873
60-64	30.843519520153162	19.11697741692819	19.08918702091505	30.9503160420036
65-69	30.749233772164956	19.168255922546916	19.20543106009492	30.877079245193208
70-74	30.800860000000004	19.239965	19.15147	30.807705000000002
75-79	30.83389257549769	19.136278397583673	19.124061748336025	30.90576727858261
80-84	30.736704779525333	19.195754796250387	19.210614289761224	30.856926134463063
85-89	30.809700037816473	19.231154053450382	19.171811742067355	30.787334166665794
90-94	30.88190720022144	19.12810292690717	19.10653265296469	30.883457219906692
95-99	30.76539158496587	19.19169283617641	19.18721199265762	30.8557035862001
100-101	30.93288002884665	19.289283679888214	19.117769145788106	30.660067145477033
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.02750167946702
2	54.41171242667841
3	57.276838390203565
4	60.158933858339466
5	60.826959972699726
6	62.72116244202222
7	62.00420089214461
8	60.95487359645584
9	62.21619226241815
10-14	61.397120187009826
15-19	61.61080604526944
20-24	61.577352448225945
25-29	61.53402013357322
30-34	61.769053927877835
35-39	61.644074894311665
40-44	61.67006772654068
45-49	61.83954759540349
50-54	61.66772712959684
55-59	61.63647421316879
60-64	61.793835562156765
65-69	61.62631301735817
70-74	61.608565000000006
75-79	61.7396598540803
80-84	61.59363091398839
85-89	61.597034204482256
90-94	61.76536442012813
95-99	61.621095171165976
100-101	61.59294717432368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	30.5
2	25.0
3	27.5
4	26.0
5	27.0
6	36.0
7	46.5
8	62.5
9	77.5
10	100.5
11	149.5
12	191.5
13	234.5
14	287.5
15	350.5
16	450.0
17	563.5
18	657.5
19	786.0
20	1011.0
21	1275.5
22	1537.5
23	1891.5
24	2370.0
25	2948.5
26	3688.5
27	4522.0
28	5457.0
29	6575.0
30	7827.5
31	9203.5
32	10649.5
33	12242.5
34	13625.5
35	14927.0
36	16416.5
37	17635.5
38	18838.0
39	20000.5
40	21190.0
41	22296.5
42	23153.0
43	24236.0
44	25515.5
45	27198.0
46	29364.5
47	32001.0
48	35500.5
49	39670.5
50	44760.5
51	51128.5
52	58303.0
53	66292.0
54	75200.0
55	84822.5
56	95287.0
57	106149.5
58	117779.5
59	129498.0
60	141113.5
61	153479.5
62	165749.5
63	177053.0
64	186978.0
65	194624.5
66	198988.5
67	199003.0
68	194915.0
69	187029.5
70	174209.0
71	157249.5
72	137698.0
73	116693.5
74	95112.0
75	74150.5
76	55315.5
77	39257.0
78	26004.0
79	16055.0
80	9360.0
81	5166.0
82	2739.0
83	1488.0
84	901.5
85	663.0
86	551.0
87	423.5
88	335.0
89	289.5
90	245.0
91	208.0
92	177.5
93	154.5
94	126.0
95	91.5
96	77.5
97	66.5
98	52.0
99	35.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19155
2	0.14025
3	0.14327499999999999
4	0.14085
5	0.147525
6	0.22039999999999998
7	0.223575
8	0.23024999999999998
9	0.20202499999999998
10-14	0.154975
15-19	0.11628000000000001
20-24	0.082445
25-29	0.09225
30-34	0.13430499999999998
35-39	0.098955
40-44	0.053215
45-49	0.070865
50-54	0.05936999999999999
55-59	0.07768
60-64	0.001425
65-69	3.7E-4
70-74	0.0
75-79	0.0135
80-84	0.030239999999999996
85-89	0.003895
90-94	0.00127
95-99	0.018824999999999998
100-101	0.023049999999999998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.4439384179013432
#Duplication Level	Relative count
1	100.0
2	0.29493790253004554
3	0.006520735940290122
4	0.002507975361650047
5	0.001003190144660019
6	0.0015047852169900284
7	0.002006380289320038
8	5.015950723300095E-4
9	0.0015047852169900284
10++	0.00852711622961016
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	381985	3.6942878	4.1392007	60-64
TTTTT	373675	3.6558115	4.117971	10-14
>>END_MODULE