##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63614325	34.0	31.0	34.0	31.0	34.0
2	32.83097275	34.0	31.0	34.0	31.0	34.0
3	32.842723	34.0	31.0	34.0	31.0	34.0
4	36.2239985	37.0	37.0	37.0	35.0	37.0
5	36.22183725	37.0	37.0	37.0	35.0	37.0
6	36.19566475	37.0	37.0	37.0	35.0	37.0
7	36.1889625	37.0	37.0	37.0	35.0	37.0
8	36.15983175	37.0	37.0	37.0	35.0	37.0
9	37.9305485	39.0	39.0	39.0	37.0	39.0
10-14	38.2412877	39.4	39.2	39.4	37.0	39.4
15-19	39.41025375	41.0	40.0	41.0	37.0	41.0
20-24	39.210093900000004	41.0	39.4	41.0	36.2	41.0
25-29	38.83726575	40.4	38.6	41.0	35.0	41.0
30-34	38.161638	40.0	37.4	41.0	34.2	41.0
35-39	37.1856839	39.0	35.2	40.8	33.0	41.0
40-44	36.24152865000001	37.0	35.0	40.0	32.2	41.0
45-49	35.36719384999999	35.0	35.0	39.0	31.4	41.0
50-54	34.12419065	35.0	33.8	36.2	29.8	39.8
55-59	34.269672650000004	35.0	35.0	35.2	31.0	39.2
60-64	33.90280944999999	35.0	35.0	35.0	31.0	37.2
65-69	33.56761125	35.0	34.4	35.0	30.6	36.0
70-74	33.269683	35.0	34.0	35.0	30.0	35.0
75-79	33.00442595	35.0	34.0	35.0	29.0	35.0
80-84	32.70750415	35.0	33.0	35.0	29.0	35.0
85-89	32.3995838	35.0	33.0	35.0	27.4	35.0
90-94	32.0588596	35.0	33.0	35.0	27.0	35.0
95-99	31.5372724	35.0	33.0	35.0	25.0	35.0
100-101	30.414220625	34.0	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8439.0
3	1513.0
4	1596.0
5	1838.0
6	2360.0
7	2801.0
8	3302.0
9	3933.0
10	3945.0
11	4178.0
12	4320.0
13	4265.0
14	4322.0
15	4514.0
16	4831.0
17	5457.0
18	6057.0
19	6992.0
20	8029.0
21	9785.0
22	11696.0
23	14344.0
24	17240.0
25	21560.0
26	26015.0
27	31685.0
28	38860.0
29	48554.0
30	60971.0
31	78114.0
32	103770.0
33	149279.0
34	249059.0
35	633963.0
36	1422582.0
37	757712.0
38	203037.0
39	38861.0
40	221.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.622408301448345	23.010251607604317	18.997275586450048	27.37006450449729
2	30.751682833732097	23.569031404971913	22.0498202366465	23.629465524649486
3	29.358990820200116	21.683494756348733	20.998432448491428	27.95908197495972
4	31.22996472089474	20.468286336227386	19.38792003402807	28.913828908849805
5	30.802313732917007	19.745110111461166	19.41720369150528	30.03537246411655
6	30.161582723655695	19.02114133256432	18.301093389447207	32.51618255433278
7	32.07231068385121	18.91463865940946	19.143316852879273	29.86973380386006
8	29.695703710532012	19.239717682139908	19.778732775965306	31.285845831362774
9	30.513268528155933	18.209553440473552	19.49040134369916	31.78677668767136
10-14	30.621952616780828	19.235257821841135	19.37147765684865	30.771311904529387
15-19	30.68687284212149	19.122866017983174	19.274247185343217	30.916013954552117
20-24	30.669591620211197	19.12371051764184	19.28571417851938	30.920983683627583
25-29	30.751054181836867	19.219443886715986	19.22400689213951	30.80549503930764
30-34	30.81968086099375	19.087130533165748	19.138745995022035	30.954442610818468
35-39	30.722147762480596	19.154610942430196	19.20370934585658	30.91953194923263
40-44	30.830352727070277	19.184871487569595	19.152727310380243	30.83204847497989
45-49	30.872900580524014	19.074345423840946	19.09624123279906	30.95651276283598
50-54	30.777790222022045	19.14066218397087	19.17450674897942	30.907040845027655
55-59	30.799876642299434	19.182367552422647	19.17565941469122	30.842096390586697
60-64	30.850173562716954	19.115688894611118	19.095373869217337	30.938763673454595
65-69	30.74065575922952	19.191336716967854	19.202026720709352	30.86598080309328
70-74	30.812728081272805	19.25289192528919	19.14613191461319	30.788248078824807
75-79	30.83108230447551	19.13998321785294	19.115441540429288	30.91349293724226
80-84	30.742549601815355	19.207686068687977	19.202390607982895	30.847373721513772
85-89	30.832542122507682	19.248910634068498	19.16640037416118	30.752146869262635
90-94	30.87266785263517	19.151274684475762	19.11091462191766	30.865142840971405
95-99	30.770971022089512	19.201414965594502	19.208250958864323	30.819363053451664
100-101	30.916458125672676	19.328221500477508	19.12349148193854	30.63182889191128
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.992472805945624
2	54.38114835838158
3	57.318072795159836
4	60.143793629744536
5	60.837686197033555
6	62.67776527798847
7	61.942044487711264
8	60.981549541894786
9	62.300045215827296
10-14	61.39326452131022
15-19	61.60288679667361
20-24	61.590575303838776
25-29	61.55654922114451
30-34	61.77412347181222
35-39	61.64167971171322
40-44	61.66240120205016
45-49	61.829413343359995
50-54	61.68483106704971
55-59	61.641973032886135
60-64	61.78893723617155
65-69	61.60663656232279
70-74	61.60097616009762
75-79	61.74457524171777
80-84	61.58992332332913
85-89	61.58468899177032
90-94	61.737810693606576
95-99	61.59033407554118
100-101	61.54828701758396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	35.5
2	23.5
3	24.0
4	21.5
5	22.5
6	29.5
7	45.5
8	63.0
9	68.0
10	79.0
11	105.0
12	126.0
13	167.5
14	231.0
15	291.5
16	365.5
17	453.5
18	566.5
19	720.0
20	931.0
21	1153.0
22	1437.0
23	1824.5
24	2315.5
25	2878.0
26	3564.5
27	4454.0
28	5363.5
29	6344.0
30	7634.5
31	9094.5
32	10488.5
33	11911.0
34	13383.0
35	14760.5
36	16180.5
37	17527.5
38	18753.5
39	19940.0
40	21043.0
41	22053.5
42	23067.5
43	24134.5
44	25483.5
45	27150.0
46	29216.0
47	32035.0
48	35454.5
49	39767.5
50	44899.0
51	51024.0
52	58220.5
53	66281.0
54	74966.0
55	84369.0
56	94951.5
57	106398.0
58	118065.0
59	129748.0
60	142165.0
61	154501.5
62	166911.5
63	178796.5
64	188762.5
65	195921.5
66	199654.0
67	199996.0
68	195692.0
69	186873.0
70	173993.5
71	157453.0
72	138063.0
73	116856.5
74	95035.5
75	73892.0
76	54765.5
77	38366.5
78	25216.0
79	15477.0
80	8735.5
81	4575.5
82	2344.5
83	1236.0
84	713.5
85	477.0
86	359.0
87	266.5
88	219.5
89	190.5
90	152.0
91	127.5
92	108.0
93	89.0
94	75.5
95	59.5
96	46.0
97	42.0
98	29.5
99	17.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16199999999999998
2	0.097825
3	0.11492500000000001
4	0.0825
5	0.070575
6	0.13165
7	0.15445
8	0.1976
9	0.20087500000000003
10-14	0.109995
15-19	0.08995
20-24	0.048555
25-29	0.06586499999999999
30-34	0.097765
35-39	0.05785
40-44	0.044105
45-49	0.026529999999999998
50-54	0.043035000000000004
55-59	0.046775000000000004
60-64	1.25E-4
65-69	3.5E-5
70-74	9.999999999999999E-6
75-79	0.006834999999999999
80-84	0.0087
85-89	3.15E-4
90-94	1.55E-4
95-99	0.014530000000000001
100-101	0.0146625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.36171966725786914
#Duplication Level	Relative count
1	100.0
2	0.26776178227055975
3	0.005014265585591007
4	0.0020057062342364027
5	5.014265585591007E-4
6	0.0010028531171182014
7	0.0025071327927955033
8	0.0010028531171182014
9	0.001504279675677302
10++	0.0040114124684728055
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	375465	3.6285846	4.2533636	60-64
TTTTT	364265	3.5702903	3.9155056	10-14
>>END_MODULE