##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55645575	34.0	31.0	34.0	31.0	34.0
2	32.7290645	34.0	31.0	34.0	31.0	34.0
3	32.77196275	34.0	31.0	34.0	31.0	34.0
4	36.15580875	37.0	37.0	37.0	35.0	37.0
5	36.158804	37.0	37.0	37.0	35.0	37.0
6	36.1425125	37.0	37.0	37.0	35.0	37.0
7	36.134267	37.0	37.0	37.0	35.0	37.0
8	36.1105995	37.0	37.0	37.0	35.0	37.0
9	37.8676005	39.0	39.0	39.0	35.0	39.0
10-14	38.15755635	39.4	38.8	39.4	35.4	39.4
15-19	39.2986423	41.0	39.8	41.0	36.4	41.0
20-24	39.08692610000001	41.0	39.0	41.0	35.8	41.0
25-29	38.7159992	40.4	38.4	41.0	35.0	41.0
30-34	38.05025115	40.0	37.2	41.0	33.8	41.0
35-39	37.06929014999999	39.0	35.2	40.8	32.8	41.0
40-44	36.05985445	37.0	35.0	40.0	31.4	41.0
45-49	35.177570200000005	35.0	35.0	39.0	31.0	41.0
50-54	33.91727899999999	34.8	33.8	36.2	29.2	39.8
55-59	34.0942519	35.0	35.0	35.2	31.0	39.2
60-64	33.7164263	35.0	34.6	35.0	30.6	37.2
65-69	33.35645195	35.0	34.0	35.0	29.6	36.0
70-74	33.066029300000004	35.0	34.0	35.0	29.0	35.0
75-79	32.75708375000001	35.0	33.2	35.0	29.0	35.0
80-84	32.43458855	35.0	33.0	35.0	27.2	35.0
85-89	32.08650264999999	35.0	33.0	35.0	27.0	35.0
90-94	31.7123515	35.0	33.0	35.0	25.2	35.0
95-99	31.1298791	35.0	33.0	35.0	23.6	35.0
100-101	29.988123125	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8339.0
3	1717.0
4	2083.0
5	2367.0
6	2757.0
7	3405.0
8	3926.0
9	4396.0
10	4625.0
11	4950.0
12	4944.0
13	4977.0
14	5131.0
15	5195.0
16	5578.0
17	6261.0
18	6935.0
19	8133.0
20	9340.0
21	11093.0
22	13312.0
23	16391.0
24	19623.0
25	24024.0
26	29054.0
27	35467.0
28	43323.0
29	53560.0
30	67533.0
31	86193.0
32	114071.0
33	160917.0
34	265599.0
35	638440.0
36	1361881.0
37	726132.0
38	199163.0
39	38912.0
40	253.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.634134735787626	22.971800651139493	19.020285499624343	27.37377911344854
2	30.742911581817246	23.569162546138482	22.05788780982809	23.63003806221618
3	29.333565956049323	21.718592929604426	21.00793386973395	27.9399072446123
4	31.19617562569545	20.49244250098983	19.415692228066934	28.895689645247792
5	30.77669847026868	19.801239792385207	19.3753286370818	30.046733100264312
6	30.216345095150203	19.0679764416667	18.29399270508442	32.421685758098675
7	32.05535444705618	18.937179399904696	19.115223824416265	29.89224232862286
8	29.622807026328204	19.29150890207864	19.798900846896665	31.286783224696496
9	30.442201860123987	18.269140991190007	19.528423150045246	31.760233998640764
10-14	30.59951864142419	19.252391619391183	19.368055377301474	30.780034361883157
15-19	30.688567826110187	19.127125871313684	19.274389804002638	30.909916498573487
20-24	30.650827681763293	19.146339029578403	19.297755606382324	30.905077682275984
25-29	30.744719715554798	19.229140175763778	19.234717866162743	30.791422242518678
30-34	30.821079492150854	19.10730627306273	19.126319344549376	30.94529489023704
35-39	30.72945134370773	19.159059258276994	19.218709350088115	30.89278004792716
40-44	30.82000317909011	19.183022858538195	19.14847101070957	30.848502951662127
45-49	30.856573353504015	19.091612117753137	19.0896566637945	30.96215786494835
50-54	30.749520394278296	19.14414981054605	19.191036255966782	30.915293539208875
55-59	30.803109983600365	19.1977728467106	19.1755945487004	30.82352262098864
60-64	30.835796714861296	19.13590571015013	19.087540581982545	30.94075699300603
65-69	30.726565	19.19565	19.2025	30.875285
70-74	30.7997	19.233705	19.176180000000002	30.790415
75-79	30.841363525535904	19.154456114117036	19.109852269265613	30.89432809108145
80-84	30.75313459171213	19.210109341111554	19.200478406429344	30.836277660746976
85-89	30.825424163646524	19.267073858967304	19.17523834009662	30.732263637289552
90-94	30.88032	19.165525	19.096455000000002	30.857699999999998
95-99	30.792945823126317	19.212049755361672	19.186956849603185	30.808047571908826
100-101	30.926048007765377	19.295610058834185	19.152097103223685	30.626244830176752
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.00791384923616
2	54.37294964403342
3	57.27347320066162
4	60.09186527094323
5	60.823431570532996
6	62.638030853248885
7	61.94759677567904
8	60.9095902510247
9	62.20243585876475
10-14	61.37955300330734
15-19	61.598484324683675
20-24	61.555905364039276
25-29	61.53614195807348
30-34	61.76637438238789
35-39	61.6222313916349
40-44	61.668506130752235
45-49	61.81873121845236
50-54	61.664813933487174
55-59	61.626632604589005
60-64	61.77655370786732
65-69	61.60185
70-74	61.590115
75-79	61.73569161661735
80-84	61.5894122524591
85-89	61.55768780093608
90-94	61.738020000000006
95-99	61.60099339503514
100-101	61.55229283794213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	30.5
2	18.5
3	22.5
4	19.5
5	27.0
6	37.5
7	41.0
8	57.0
9	68.5
10	85.5
11	109.0
12	141.5
13	176.5
14	222.0
15	277.5
16	333.5
17	433.5
18	569.0
19	731.0
20	931.5
21	1118.0
22	1421.5
23	1858.5
24	2291.5
25	2863.5
26	3631.0
27	4477.0
28	5407.5
29	6540.0
30	7795.0
31	9066.5
32	10486.5
33	12093.5
34	13593.5
35	15012.5
36	16363.5
37	17555.5
38	18911.5
39	20167.0
40	21159.0
41	22202.0
42	23088.5
43	24077.5
44	25381.0
45	27098.0
46	29413.5
47	32038.0
48	35523.0
49	39798.0
50	44710.5
51	50866.5
52	58296.0
53	66487.5
54	75611.5
55	85676.5
56	95667.5
57	106044.5
58	117521.0
59	129464.0
60	141775.5
61	154490.5
62	167023.0
63	178033.5
64	187340.0
65	194751.0
66	199234.5
67	199611.5
68	195604.5
69	187181.0
70	174172.5
71	157422.5
72	137342.5
73	115755.0
74	94587.5
75	73712.0
76	54306.5
77	38447.5
78	25660.0
79	15824.5
80	9109.5
81	4992.5
82	2704.5
83	1498.5
84	958.0
85	661.5
86	495.5
87	400.5
88	321.5
89	268.0
90	221.0
91	180.0
92	162.0
93	138.0
94	112.0
95	90.5
96	81.0
97	66.0
98	46.0
99	39.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.12405000000000001
3	0.12440000000000001
4	0.10915
5	0.108275
6	0.0948
7	0.1092
8	0.1413
9	0.12762500000000002
10-14	0.07241500000000001
15-19	0.06039
20-24	0.037365
25-29	0.048235
30-34	0.06874999999999999
35-39	0.042065
40-44	0.03429
45-49	0.023215
50-54	0.035075
55-59	0.037415000000000004
60-64	2.65E-4
65-69	0.0
70-74	0.0
75-79	0.00862
80-84	0.009705
85-89	5.65E-4
90-94	0.0
95-99	0.01158
100-101	0.0177375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.29288723841320025
#Duplication Level	Relative count
1	100.0
2	0.24763021890711862
3	0.003508930227428806
4	5.012757467755438E-4
5	0.0010025514935510875
6	0.0
7	0.0
8	0.0
9	5.012757467755438E-4
10++	0.002005102987102175
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	381350	3.6782892	4.205486	60-64
TTTTT	372440	3.64654	4.082283	10-14
>>END_MODULE