##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49143525	34.0	31.0	34.0	31.0	34.0
2	32.6711365	34.0	31.0	34.0	31.0	34.0
3	32.7085965	34.0	31.0	34.0	31.0	34.0
4	36.088495	37.0	37.0	37.0	35.0	37.0
5	36.0872345	37.0	37.0	37.0	35.0	37.0
6	36.081318	37.0	37.0	37.0	35.0	37.0
7	36.075447	37.0	37.0	37.0	35.0	37.0
8	36.06063025	37.0	37.0	37.0	35.0	37.0
9	37.83287625	39.0	39.0	39.0	35.0	39.0
10-14	38.12475965	39.4	38.6	39.4	35.2	39.4
15-19	39.2637253	41.0	39.6	41.0	36.0	41.0
20-24	39.0601972	41.0	39.0	41.0	35.6	41.0
25-29	38.6832865	40.2	38.4	41.0	35.0	41.0
30-34	37.9980596	40.0	37.2	41.0	33.4	41.0
35-39	37.03063055	38.8	35.2	40.8	32.6	41.0
40-44	36.023795199999995	37.0	35.0	40.0	31.2	41.0
45-49	35.10650665	35.0	35.0	38.8	30.6	41.0
50-54	33.84563415000001	34.8	33.4	36.2	28.6	39.8
55-59	34.05138635	35.0	35.0	35.2	31.0	39.2
60-64	33.6686769	35.0	34.4	35.0	30.2	37.2
65-69	33.3379122	35.0	34.0	35.0	29.6	36.0
70-74	33.03784674999999	35.0	34.0	35.0	29.0	35.0
75-79	32.7278323	35.0	33.2	35.0	28.4	35.0
80-84	32.395687499999994	35.0	33.0	35.0	27.0	35.0
85-89	32.0896275	35.0	33.0	35.0	27.0	35.0
90-94	31.69766655	35.0	33.0	35.0	25.0	35.0
95-99	31.11964365	35.0	33.0	35.0	23.4	35.0
100-101	30.039827875	34.0	31.0	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8052.0
3	1777.0
4	1963.0
5	2362.0
6	2812.0
7	3441.0
8	4168.0
9	4582.0
10	4874.0
11	5088.0
12	5150.0
13	5284.0
14	5141.0
15	5305.0
16	5657.0
17	6349.0
18	7082.0
19	7986.0
20	9592.0
21	11575.0
22	13757.0
23	16309.0
24	20278.0
25	24727.0
26	29627.0
27	36637.0
28	44747.0
29	55911.0
30	69309.0
31	88661.0
32	117648.0
33	164784.0
34	267246.0
35	626934.0
36	1344132.0
37	732093.0
38	199280.0
39	39428.0
40	252.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.597662665840218	22.960459516477062	19.050248398462163	27.39162941922056
2	30.724712113107948	23.60227829295881	22.063516122041747	23.60949347189149
3	29.307894355835412	21.744684752294102	21.016163042176807	27.931257849693676
4	31.21655851833445	20.531391406115358	19.412416884766916	28.839633190783275
5	30.721227823955516	19.823643858500215	19.46964887605967	29.985479441484603
6	30.172491031577557	19.045515588562264	18.314301974679694	32.46769140518049
7	32.10640975224142	18.91352893453824	19.130731030111665	29.849330283108678
8	29.57946199495683	19.3648868691279	19.833553312810817	31.222097823104455
9	30.43257802406932	18.279338456602297	19.54021722753831	31.74786629179007
10-14	30.580068441120567	19.275761119405622	19.385937144380126	30.758233295093685
15-19	30.66580704618021	19.146055516450573	19.298948447180862	30.88918899018836
20-24	30.62326629316851	19.169584058388775	19.32385858653346	30.88329106190925
25-29	30.7217077633385	19.240082681267097	19.24659888448402	30.791610670910387
30-34	30.805818096540534	19.10081670536707	19.167994226004808	30.925370972087592
35-39	30.727457706726497	19.176409624088908	19.208570535264382	30.887562133920216
40-44	30.797167325230546	19.200032403143105	19.185701012998262	30.81709925862809
45-49	30.84283862704152	19.1075816889734	19.104941572676275	30.9446381113088
50-54	30.717615682314992	19.16953571862176	19.2231898070633	30.889658791999953
55-59	30.796136442415566	19.22964567687464	19.173786085216204	30.800431795493587
60-64	30.826005000000002	19.13353	19.11515	30.925314999999998
65-69	30.74073	19.196365	19.214190000000002	30.848715
70-74	30.783135	19.2638	19.190034999999998	30.76303
75-79	30.833739666991733	19.15800532640426	19.133765307012247	30.874489699591763
80-84	30.739902292386322	19.217459067030042	19.234959535167565	30.80767910541607
85-89	30.81251	19.275515	19.19113	30.720845
90-94	30.88066	19.15521	19.11417	30.84996
95-99	30.77557773514971	19.224519765508415	19.212374683529113	30.787527815812755
100-101	30.924579118013458	19.34199634849361	19.131320979811715	30.60210355368122
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	57.989292085060775
2	54.33420558499944
3	57.23915220552909
4	60.056191709117726
5	60.706707265440116
6	62.64018243675804
7	61.95574003535009
8	60.80155981806128
9	62.1804443158594
10-14	61.33830173621425
15-19	61.55499603636857
20-24	61.50655735507776
25-29	61.51331843424889
30-34	61.731189068628126
35-39	61.61501984064671
40-44	61.61426658385864
45-49	61.78747673835032
50-54	61.60727447431494
55-59	61.59656823790915
60-64	61.75132
65-69	61.589445
70-74	61.546164999999995
75-79	61.70822936658349
80-84	61.54758139780239
85-89	61.533355
90-94	61.73062
95-99	61.56310555096247
100-101	61.52668267169468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	20.5
2	14.5
3	20.0
4	22.0
5	22.0
6	23.0
7	30.0
8	45.0
9	60.0
10	69.0
11	80.0
12	104.0
13	136.0
14	194.0
15	241.5
16	306.0
17	395.5
18	502.0
19	659.5
20	880.0
21	1128.0
22	1418.0
23	1821.0
24	2271.5
25	2865.0
26	3674.5
27	4470.5
28	5450.5
29	6677.5
30	7853.5
31	9189.0
32	10667.5
33	12139.5
34	13623.5
35	15105.5
36	16592.5
37	17933.0
38	19175.5
39	20276.0
40	21311.5
41	22422.0
42	23372.0
43	24201.0
44	25540.5
45	27552.5
46	29709.5
47	32423.5
48	35802.0
49	39998.0
50	45165.5
51	51215.0
52	58629.5
53	66795.0
54	75492.5
55	84995.0
56	95618.0
57	106665.0
58	117628.5
59	129766.5
60	142073.5
61	153983.5
62	166161.0
63	177783.0
64	187349.5
65	195117.0
66	199597.5
67	198937.0
68	194629.5
69	186156.0
70	173461.0
71	156856.5
72	136989.0
73	115853.0
74	94426.0
75	73626.0
76	54465.0
77	38131.5
78	25430.0
79	15856.0
80	9148.5
81	5002.5
82	2627.5
83	1506.5
84	924.5
85	627.0
86	510.0
87	411.5
88	339.0
89	274.0
90	244.0
91	224.5
92	170.0
93	140.5
94	121.5
95	94.0
96	83.5
97	69.0
98	53.0
99	38.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.256025
2	0.210375
3	0.19515
4	0.19880000000000003
5	0.21045
6	0.10785
7	0.127575
8	0.14220000000000002
9	0.10935
10-14	0.064465
15-19	0.041159999999999995
20-24	0.019139999999999997
25-29	0.018465
30-34	0.04841
35-39	0.01838
40-44	0.0097
45-49	0.004405
50-54	0.007619999999999999
55-59	0.00822
60-64	0.0
65-69	0.0
70-74	0.0
75-79	7.999999999999999E-5
80-84	0.002675
85-89	0.0
90-94	0.0
95-99	6.749999999999999E-4
100-101	1.75E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.36679459502678363
#Duplication Level	Relative count
1	100.0
2	0.2999327909799476
3	0.007021837915918506
4	0.0025077992556851807
5	5.015598511370362E-4
6	5.015598511370362E-4
7	0.0
8	0.0010031197022740725
9	0.0
10++	5.015598511370362E-4
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	389145	3.739521	4.2544765	60-64
TTTTT	373625	3.6425934	3.9629278	10-14
>>END_MODULE