##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R1_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.763748	34.0	31.0	34.0	31.0	34.0
2	33.01093825	34.0	31.0	34.0	31.0	34.0
3	33.0269115	34.0	31.0	34.0	31.0	34.0
4	36.43508525	37.0	37.0	37.0	35.0	37.0
5	36.3724045	37.0	37.0	37.0	35.0	37.0
6	36.342738	37.0	37.0	37.0	35.0	37.0
7	36.346063	37.0	37.0	37.0	35.0	37.0
8	36.32691325	37.0	37.0	37.0	35.0	37.0
9	38.1281605	39.0	39.0	39.0	37.0	39.0
10-14	38.3911619	39.4	38.6	39.4	37.0	39.4
15-19	39.5063047	41.0	39.8	41.0	37.0	41.0
20-24	39.26773635	40.8	39.0	41.0	36.0	41.0
25-29	38.6330047	40.0	38.4	41.0	34.4	41.0
30-34	37.799766899999995	40.0	36.8	41.0	33.0	41.0
35-39	36.905866200000006	38.6	35.0	40.6	31.8	41.0
40-44	35.91575855	36.8	35.0	40.0	31.2	41.0
45-49	35.357201499999995	35.0	35.0	39.2	31.2	41.0
50-54	34.52052905000001	35.0	34.8	36.6	30.8	40.4
55-59	33.644842749999995	35.0	34.0	35.0	29.0	38.8
60-64	33.508869149999995	35.0	34.0	35.0	30.0	37.0
65-69	32.8902967	35.0	33.0	35.0	27.6	35.8
70-74	32.67111765	35.0	33.0	35.0	27.4	35.0
75-79	32.10662000000001	34.4	32.6	35.0	26.4	35.0
80-84	32.383354749999995	35.0	33.0	35.0	27.0	35.0
85-89	32.04752599999999	35.0	33.0	35.0	27.0	35.0
90-94	31.634482350000003	35.0	33.0	35.0	25.6	35.0
95-99	31.0073379	35.0	32.6	35.0	23.0	35.0
100-101	29.403816875	34.0	30.5	35.0	9.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31.0
3	10.0
4	36.0
5	105.0
6	339.0
7	907.0
8	2319.0
9	3565.0
10	4595.0
11	5242.0
12	5660.0
13	5844.0
14	6094.0
15	6550.0
16	7382.0
17	7730.0
18	8436.0
19	9072.0
20	9851.0
21	11002.0
22	12997.0
23	15571.0
24	19530.0
25	24627.0
26	30669.0
27	38914.0
28	48797.0
29	60503.0
30	77077.0
31	98948.0
32	133631.0
33	190209.0
34	314946.0
35	709448.0
36	1299869.0
37	635696.0
38	169062.0
39	24660.0
40	76.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.45570774931223	22.576792722038878	19.373455606227534	27.594043922421356
2	30.446050000000003	22.954749999999997	22.772524999999998	23.826675
3	28.726875000000003	21.7695	21.982	27.521625
4	30.672300000000003	20.688375	20.211399999999998	28.427925
5	30.793975	19.766624999999998	19.5074	29.932
6	29.96705	19.251974999999998	18.657575	32.123400000000004
7	31.903274999999997	19.069924999999998	19.2166	29.8102
8	29.692675	19.30135	19.791175	31.214799999999997
9	30.27355	18.432775	19.763925	31.52975
10-14	30.558047781266893	19.29368701116182	19.446712225397114	30.701552982174174
15-19	30.63836	19.188525000000002	19.3481	30.825015
20-24	30.636604078246517	19.202742771108223	19.37263345916551	30.78801969147975
25-29	30.92320622766077	19.237521970153843	19.245327246850902	30.593944555334485
30-34	31.011844199603626	19.152062755549686	19.160061266896168	30.67603177795052
35-39	30.810955893380843	19.229449548308718	19.235825801561298	30.723768756749138
40-44	30.95800550984959	19.222701896356487	19.171320501351612	30.64797209244231
45-49	30.883325	19.160235	19.168995	30.787445
50-54	30.856135000000002	19.230315	19.213820000000002	30.69973
55-59	30.94096	19.27783	19.166775	30.614435
60-64	30.822250619882702	19.130531532696075	19.17883200272431	30.86838584469691
65-69	30.83514245461162	19.261758504886902	19.246621792924778	30.656477247576703
70-74	30.86915	19.271235	19.1551	30.704515
75-79	30.85504	19.16019	19.174715	30.810055
80-84	30.768043827103426	19.21021332392385	19.244893880546783	30.77684896842594
85-89	30.84898502850605	19.233552675496192	19.182299443979943	30.735162852017822
90-94	30.878223249316655	19.13921198539991	19.156699728352393	30.825865036931045
95-99	30.824800084162458	19.181045086516527	19.221656272363152	30.77249855695786
100-101	31.24268671600752	19.273644727616773	19.007644627866735	30.476023928508972
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.04975167173359
2	54.272725
3	56.2485
4	59.100224999999995
5	60.725975
6	62.09045
7	61.713474999999995
8	60.907475
9	61.80330000000001
10-14	61.25960076344107
15-19	61.463375
20-24	61.424623769726274
25-29	61.51715078299526
30-34	61.68787597755414
35-39	61.53472465012998
40-44	61.60597760229191
45-49	61.67077
50-54	61.555865
55-59	61.555395000000004
60-64	61.69063646457962
65-69	61.491619702188316
70-74	61.573665
75-79	61.665095
80-84	61.54489279552937
85-89	61.584147880523865
90-94	61.704088286247696
95-99	61.597298641120325
100-101	61.71871064451649
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	18.0
2	13.5
3	12.5
4	16.5
5	20.0
6	18.5
7	22.0
8	33.0
9	42.5
10	52.5
11	77.5
12	103.5
13	114.5
14	155.0
15	207.5
16	276.0
17	377.5
18	492.5
19	616.0
20	813.5
21	1033.0
22	1261.5
23	1628.5
24	2170.5
25	2781.5
26	3465.0
27	4315.0
28	5297.5
29	6416.0
30	7618.5
31	8935.5
32	10493.0
33	11997.0
34	13446.5
35	15068.5
36	16462.5
37	17790.5
38	19052.0
39	20237.0
40	21369.5
41	22467.5
42	23546.5
43	24442.5
44	25664.0
45	27575.5
46	29936.5
47	32587.5
48	36019.0
49	40637.0
50	46216.5
51	52538.5
52	59723.0
53	67899.5
54	76832.0
55	86349.0
56	96472.5
57	107726.5
58	119473.5
59	131168.5
60	143429.0
61	155798.0
62	167652.0
63	178526.0
64	187631.5
65	194549.0
66	198478.5
67	198568.0
68	194175.5
69	185148.0
70	171660.0
71	154497.5
72	135242.0
73	114661.0
74	93124.5
75	72121.0
76	53516.0
77	37808.5
78	25089.5
79	15492.5
80	9012.5
81	5023.0
82	2727.0
83	1483.0
84	891.0
85	608.5
86	434.0
87	315.0
88	222.0
89	153.5
90	114.0
91	81.5
92	56.0
93	41.5
94	26.5
95	13.0
96	9.5
97	8.0
98	3.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02145
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	1.4E-4
15-19	0.0
20-24	4.0500000000000003E-4
25-29	0.003545
30-34	0.0439
35-39	0.019655000000000002
40-44	0.002715
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.011325
65-69	0.01131
70-74	0.0
75-79	0.0
80-84	0.001605
85-89	0.006305
90-94	0.015684999999999998
95-99	0.0029200000000000003
100-101	3.7500000000000003E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.2759213624117081
#Duplication Level	Relative count
1	100.0
2	0.23658554923435504
3	0.0055136462745294604
4	0.0020049622816470766
5	5.012405704117692E-4
6	0.0010024811408235383
7	0.0
8	0.0010024811408235383
9	0.0
10++	0.0015037217112353074
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	372340	3.5803585	4.139791	70-74
TTTTT	363585	3.4986541	3.9478962	95-97
>>END_MODULE