##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002004_Nocton-Corn_CGTACG_L002_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	61	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9058125	34.0	31.0	34.0	31.0	34.0
2	33.13341075	34.0	33.0	34.0	31.0	34.0
3	33.157081	34.0	34.0	34.0	31.0	34.0
4	36.511803	37.0	37.0	37.0	35.0	37.0
5	36.455566	37.0	37.0	37.0	35.0	37.0
6	36.425079	37.0	37.0	37.0	35.0	37.0
7	36.416844	37.0	37.0	37.0	35.0	37.0
8	36.40812275	37.0	37.0	37.0	35.0	37.0
9	38.20887975	39.0	39.0	39.0	37.0	39.0
10-14	38.48317295	39.4	39.2	39.4	37.0	39.4
15-19	39.65374154999999	41.0	40.0	41.0	37.0	41.0
20-24	39.42009564999999	41.0	39.4	41.0	36.6	41.0
25-29	38.97996145	40.2	38.6	41.0	35.2	41.0
30-34	38.243937450000004	40.0	37.4	41.0	34.2	41.0
35-39	37.2187817	38.8	35.2	40.6	32.8	41.0
40-44	36.37605915	37.0	35.0	40.0	32.4	41.0
45-49	35.70673565	35.2	35.0	39.2	32.8	41.0
50-54	34.8562004	35.0	35.0	37.0	31.6	40.4
55-59	34.20476115	35.0	34.4	35.2	31.0	39.2
60-64	33.687990199999994	35.0	34.0	35.0	30.4	37.2
65-69	33.27622145	35.0	33.4	35.0	29.4	35.8
70-74	32.96989965	35.0	33.0	35.0	29.0	35.0
75-79	32.2552824	34.4	32.6	35.0	27.4	35.0
80-84	32.504787900000004	35.0	33.0	35.0	28.6	35.0
85-89	32.12975805	35.0	33.0	35.0	27.0	35.0
90-94	31.698935600000006	35.0	33.0	35.0	25.6	35.0
95-99	31.126568650000003	35.0	33.0	35.0	23.8	35.0
100-101	29.885366375	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29.0
3	9.0
4	30.0
5	103.0
6	302.0
7	812.0
8	1999.0
9	3140.0
10	3948.0
11	4503.0
12	4710.0
13	4941.0
14	5350.0
15	5903.0
16	6477.0
17	7344.0
18	7881.0
19	8509.0
20	9447.0
21	10376.0
22	11911.0
23	14392.0
24	17635.0
25	22580.0
26	27895.0
27	34609.0
28	43045.0
29	53191.0
30	66835.0
31	86115.0
32	114661.0
33	162042.0
34	271565.0
35	674424.0
36	1380630.0
37	706363.0
38	195471.0
39	30732.0
40	91.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.533057430781234	22.538633482359714	19.316528677890272	27.611780408968784
2	30.512525	22.919275	22.7485	23.819699999999997
3	28.7321	21.69715	21.925900000000002	27.644849999999998
4	30.714599999999997	20.6567	20.1128	28.5159
5	30.847625	19.723825	19.49585	29.9327
6	30.0441	19.19505	18.62285	32.138
7	31.9126	19.029825	19.2075	29.850074999999997
8	29.709025	19.290675	19.757075	31.243225000000002
9	30.27455	18.386625000000002	19.758075	31.580750000000002
10-14	30.5985805985806	19.262869262869263	19.42990442990443	30.70864570864571
15-19	30.65326	19.18164	19.325105	30.839995
20-24	30.623715	19.19332	19.352485	30.830479999999998
25-29	30.749578266813987	19.24388891275117	19.267280060086946	30.739252760347895
30-34	30.769956158946393	19.15528117481117	19.191995899797163	30.882766766445275
35-39	30.723332026961632	19.174144381960748	19.29033761848922	30.812185972588395
40-44	30.808679248528335	19.206535906666247	19.21168095914578	30.773103885659637
45-49	30.817014999999998	19.111025	19.187070000000002	30.88489
50-54	30.74292	19.18393	19.23723	30.83592
55-59	30.805505	19.191985	19.20749	30.79502
60-64	30.84152004880714	19.110887382796925	19.177939178758567	30.869653389637364
65-69	30.729363652284107	19.18209583284583	19.24764897808874	30.840891536781317
70-74	30.864710000000002	19.224585	19.16732	30.743385
75-79	30.842065	19.13891	19.148390000000003	30.870635
80-84	30.78276202728886	19.201024426126708	19.21980063813119	30.79641290845324
85-89	30.849066989610286	19.206075480235167	19.17436899773253	30.770488532422018
90-94	30.888053662693782	19.113006991411606	19.14091420178824	30.85802514410637
95-99	30.851053083185963	19.161609179560386	19.172996214423517	30.81434152283013
100-101	31.08484823559986	19.153126434020045	19.043136198012924	30.718889132367167
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	58.14483783975002
2	54.332225
3	56.37695
4	59.2305
5	60.780325000000005
6	62.1821
7	61.762675
8	60.95225
9	61.8553
10-14	61.3072263072263
15-19	61.493255000000005
20-24	61.454195
25-29	61.488831027161886
30-34	61.652722925391664
35-39	61.535517999550024
40-44	61.58178313418797
45-49	61.701905000000004
50-54	61.57884
55-59	61.600525
60-64	61.71117343844451
65-69	61.57025518906543
70-74	61.608095
75-79	61.7127
80-84	61.579174935742095
85-89	61.6195555220323
90-94	61.746078806800156
95-99	61.6653946060161
100-101	61.803737367967024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33.0
1	20.5
2	11.5
3	14.5
4	10.5
5	18.5
6	25.5
7	26.0
8	31.0
9	37.0
10	53.0
11	71.0
12	91.5
13	127.5
14	174.5
15	234.0
16	296.0
17	369.5
18	481.5
19	629.0
20	819.5
21	1067.5
22	1381.0
23	1741.0
24	2133.0
25	2755.5
26	3482.0
27	4325.0
28	5361.0
29	6486.5
30	7655.5
31	9013.0
32	10516.0
33	12140.5
34	13679.0
35	14906.5
36	16272.5
37	17748.5
38	19047.5
39	20245.5
40	21184.5
41	22014.5
42	23247.5
43	24397.0
44	25655.5
45	27444.0
46	29681.0
47	32530.5
48	36243.0
49	40600.0
50	45908.0
51	52322.5
52	59720.0
53	67909.5
54	76603.5
55	86520.0
56	96896.0
57	107555.5
58	118780.0
59	130491.5
60	142810.5
61	155284.5
62	167090.5
63	177639.0
64	187044.0
65	194118.0
66	198087.0
67	197781.0
68	193670.5
69	185523.5
70	172134.0
71	154986.0
72	135409.0
73	114901.0
74	94146.0
75	73555.0
76	54380.5
77	38244.0
78	25617.5
79	16088.0
80	9476.0
81	5344.0
82	2891.5
83	1569.5
84	952.0
85	609.5
86	428.0
87	318.5
88	216.5
89	150.5
90	104.0
91	77.0
92	59.0
93	32.5
94	15.0
95	13.0
96	8.0
97	2.5
98	2.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.249999999999999E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	9.999999999999999E-5
15-19	0.0
20-24	0.0
25-29	0.004905
30-34	0.053724999999999995
35-39	0.015695
40-44	0.00102
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.04742
65-69	0.042935
70-74	0.0
75-79	0.0
80-84	0.006455
85-89	0.036215
90-94	0.061669999999999996
95-99	0.01787
100-101	0.0024875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.30340139454676535
#Duplication Level	Relative count
1	100.0
2	0.2597037972906577
3	0.00601630418433956
4	0.0025067934101414835
5	0.0010027173640565934
6	0.0
7	0.0010027173640565934
8	0.0
9	5.013586820282967E-4
10++	0.0010027173640565934
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	362730	3.5013022	3.9293158	70-74
TTTTT	357530	3.4377184	3.6875234	8
>>END_MODULE