##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_R2_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	31794963	
Filtered Sequences	0	
Sequence length	30-101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.740821022499695	34.0	31.0	34.0	31.0	34.0
2	32.849616211221885	34.0	31.0	34.0	31.0	34.0
3	32.881444365889024	34.0	31.0	34.0	31.0	34.0
4	36.25186571218844	37.0	37.0	37.0	35.0	37.0
5	36.24796987497674	37.0	37.0	37.0	35.0	37.0
6	36.242501555985456	37.0	37.0	37.0	35.0	37.0
7	36.243427614619335	37.0	37.0	37.0	35.0	37.0
8	36.22981234480443	37.0	37.0	37.0	35.0	37.0
9	37.99323622424093	39.0	39.0	39.0	37.0	39.0
10-14	38.27091981204696	39.4	38.8	39.4	35.4	39.4
15-19	39.42897804913313	41.0	39.8	41.0	36.8	41.0
20-24	39.193817372896454	41.0	39.0	41.0	35.6	41.0
25-29	38.90086737952801	40.2	38.4	41.0	35.0	41.0
30-34	38.323028178668	40.0	37.4	41.0	34.2	41.0
35-39	37.42052804446253	39.0	35.2	40.8	33.0	41.0
40-44	36.58639690698249	37.4	35.0	40.0	32.6	41.0
45-49	35.741322215944145	35.2	35.0	39.4	31.6	41.0
50-54	34.529261937932716	35.0	34.0	37.0	30.0	39.8
55-59	34.73876099229267	35.0	35.0	35.4	31.2	39.6
60-64	34.40656520121303	35.0	35.0	35.0	31.0	38.0
65-69	34.120833759934804	35.0	34.4	35.0	31.0	36.4
70-74	33.89269702396354	35.0	34.0	35.0	31.0	35.4
75-79	33.68534001750202	35.0	34.0	35.0	30.4	35.0
80-84	33.527984621471276	35.0	33.6	35.0	30.0	35.0
85-89	33.40784715252697	35.0	33.0	35.0	30.0	35.0
90-94	33.30481588595512	35.0	33.0	35.0	29.0	35.0
95-99	33.33696722063574	35.0	33.0	35.0	29.4	35.0
100-101	33.03724813857923	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	2.0
16	20.0
17	72.0
18	498.0
19	2383.0
20	7843.0
21	18183.0
22	37267.0
23	66745.0
24	107421.0
25	157130.0
26	216271.0
27	284029.0
28	364752.0
29	455258.0
30	569685.0
31	722006.0
32	949123.0
33	1333315.0
34	2169523.0
35	5110056.0
36	1.0789593E7
37	6280951.0
38	1856108.0
39	293873.0
40	2853.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.71496809101492	22.58163344929824	17.755164552322327	26.94823390736451
2	30.302581739319628	23.27615500482563	21.425713315739856	24.995549940114888
3	28.886588889817546	21.43974440546441	21.477652134133404	28.196014570584644
4	30.938231170705212	20.512026138465476	19.916354267940978	28.633388422888334
5	30.639171171256198	20.462606991374628	19.632460099679037	29.265761737690145
6	29.627107452244484	19.718599882597623	18.48592212871184	32.16837053644605
7	31.791674958566716	19.246591537463786	19.394157730423707	29.567575773545784
8	29.182540717678336	19.66878030491708	19.981343679845086	31.167335297559497
9	30.081627120487127	18.6956525569563	19.95011717922588	31.27260314333069
10-14	30.25878190004963	19.610985029905027	19.739403378648056	30.390829691397286
15-19	30.255836207858593	19.571123599364842	19.718471405534967	30.454568787241598
20-24	30.157323071115716	19.65423529471857	19.81314696038216	30.37529467378355
25-29	30.234590703296547	19.740459905683224	19.742455253076603	30.282494137943623
30-34	30.24935516090116	19.633334060272016	19.700836108753318	30.416474670073505
35-39	30.182588921980592	19.725188930410724	19.780553060921502	30.311669086687175
40-44	30.26776083418176	19.740795558398382	19.706659726084844	30.28478388133501
45-49	30.263061097359895	19.656335431912687	19.69478440316066	30.38581906756676
50-54	30.14913532084767	19.741694640791206	19.78878728755705	30.320382750804075
55-59	30.233353888173475	19.772342517479384	19.73725741122199	30.25704618312516
60-64	30.237255925664925	19.69684005730113	19.719178287016607	30.346725730017337
65-69	30.119195457151537	19.79157868145395	19.82319814953894	30.266027711855575
70-74	30.20088195443901	19.831817463405148	19.762021959743308	30.205278622412536
75-79	30.194053967039075	19.760388800629165	19.75783970840287	30.287717523928887
80-84	30.065038599030995	19.853744104424788	19.866623501606238	30.214593794937976
85-89	30.135422960268098	19.89982873551403	19.8277844815294	30.13696382268847
90-94	30.090912389316205	19.847735298846274	19.83245359428691	30.228898717550614
95-99	29.911342668615966	19.961802273928033	19.981202761712876	30.145652295743126
100-101	30.283863404729427	19.894942708803022	19.72785683486955	30.093337051598002
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.663201998379435
2	55.29813167943451
3	57.08260346040218
4	59.57161959359355
5	59.90493290894634
6	61.795477988690536
7	61.3592507321125
8	60.34987601523783
9	61.35423026381782
10-14	60.64961159144692
15-19	60.710404995100184
20-24	60.53261774489926
25-29	60.51708484124018
30-34	60.66582983097467
35-39	60.49425800866777
40-44	60.552544715516774
45-49	60.64888016492665
50-54	60.46951807165174
55-59	60.49040007129863
60-64	60.583981655682265
65-69	60.38522316900712
70-74	60.40616057685154
75-79	60.48177149096796
80-84	60.279632393968974
85-89	60.272386782956566
90-94	60.319811106866815
95-99	60.05699496435909
100-101	60.37720045632743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	711.0
1	521.5
2	321.5
3	305.5
4	314.5
5	347.5
6	395.5
7	454.5
8	533.0
9	648.5
10	801.0
11	1024.5
12	1318.5
13	1629.5
14	1979.5
15	2460.0
16	3119.0
17	3905.0
18	4854.5
19	5981.5
20	7285.5
21	8965.5
22	11008.5
23	13498.5
24	16634.0
25	20804.5
26	25575.0
27	31054.0
28	37843.0
29	46165.5
30	56118.0
31	67202.0
32	80036.0
33	95026.0
34	110528.5
35	126650.0
36	143758.5
37	161018.0
38	178320.5
39	195261.0
40	210899.5
41	225819.0
42	240988.5
43	255900.0
44	271679.0
45	288680.5
46	308219.0
47	332550.0
48	362064.5
49	397405.5
50	438679.0
51	489094.5
52	548083.5
53	613207.0
54	683776.5
55	760330.5
56	843038.5
57	929467.5
58	1020140.5
59	1113197.0
60	1205003.0
61	1298183.0
62	1387902.0
63	1463797.5
64	1521529.5
65	1554377.0
66	1556935.5
67	1529659.5
68	1467925.0
69	1372171.5
70	1250979.0
71	1110421.5
72	957005.0
73	798038.5
74	643758.5
75	502880.0
76	379758.5
77	275237.0
78	190139.0
79	125353.5
80	78973.0
81	47119.0
82	26569.5
83	14446.0
84	8093.5
85	4733.0
86	2833.5
87	1852.5
88	1288.0
89	831.0
90	532.0
91	376.0
92	248.5
93	153.0
94	109.5
95	76.5
96	50.5
97	37.5
98	30.5
99	29.5
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01024061578558843
3	0.014505442261404738
4	0.023047675822110567
5	0.024966218705774243
6	0.032259826815964524
7	0.0375468277789787
8	0.046699220879734944
9	0.04050641606345005
10-14	0.015592406885329604
15-19	0.0059349023302842025
20-24	0.002583428073182535
25-29	0.0031470393596620947
30-34	0.011473779788195675
35-39	0.0010179630555474245
40-44	3.159778996209624E-4
45-49	3.7359207788754016E-5
50-54	3.273263492801275E-4
55-59	6.614153212837312E-4
60-64	0.0
65-69	1.116933597308356E-4
70-74	0.0
75-79	6.425742532140183E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	23275.0
32-33	23820.0
34-35	24920.0
36-37	25239.0
38-39	24654.0
40-41	25282.0
42-43	25270.0
44-45	24508.0
46-47	25243.0
48-49	25262.0
50-51	19681.0
52-53	17379.0
54-55	20597.0
56-57	23083.0
58-59	25535.0
60-61	27368.0
62-63	29123.0
64-65	31600.0
66-67	33975.0
68-69	36540.0
70-71	39930.0
72-73	43432.0
74-75	47390.0
76-77	52758.0
78-79	57977.0
80-81	63999.0
82-83	71928.0
84-85	80713.0
86-87	94888.0
88-89	113455.0
90-91	140124.0
92-93	178443.0
94-95	237499.0
96-97	323793.0
98-99	489788.0
100-101	2.9246492E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	3.7808071318635155
#Duplication Level	Relative count
1	100.0
2	3.726824201506102
3	0.10594650740067516
4	0.010906258114775382
5	0.006232147494157362
6	0.005193456245131135
7	0.001038691249026227
8	0.0015580368735393405
9	0.0
10++	0.011425603739288496
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3176080	3.4615064	3.7855513	95-97
AAAAA	3203110	3.4360929	3.6602595	95-97
>>END_MODULE