##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_R1_filtered.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	31794963	
Filtered Sequences	0	
Sequence length	30-101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8000162164051	34.0	31.0	34.0	31.0	34.0
2	33.01691227003472	34.0	31.0	34.0	31.0	34.0
3	33.048745991621374	34.0	31.0	34.0	31.0	34.0
4	36.443181110165156	37.0	37.0	37.0	35.0	37.0
5	36.379883662704685	37.0	37.0	37.0	35.0	37.0
6	36.349948103414995	37.0	37.0	37.0	35.0	37.0
7	36.34686503645247	37.0	37.0	37.0	35.0	37.0
8	36.32946680894077	37.0	37.0	37.0	35.0	37.0
9	38.120379004687	39.0	39.0	39.0	37.0	39.0
10-14	38.364884802665124	39.4	38.6	39.4	36.2	39.4
15-19	39.516634593976406	41.0	39.8	41.0	37.0	41.0
20-24	39.2491485333699	40.8	39.0	41.0	35.8	41.0
25-29	37.6187602482821	40.0	37.4	41.0	28.6	41.0
30-34	37.14245391161984	39.6	36.0	41.0	31.2	41.0
35-39	36.99478318016837	38.6	35.0	40.4	32.2	41.0
40-44	35.28068410799144	36.4	34.6	40.0	26.6	41.0
45-49	35.44472593172937	35.0	34.8	39.0	30.8	41.0
50-54	34.81656151045969	35.0	34.6	37.0	30.4	40.6
55-59	34.1419329348099	35.0	34.0	35.2	29.8	39.2
60-64	34.02568226331415	35.0	34.0	35.0	30.2	37.6
65-69	33.73160177929231	35.0	33.4	35.0	29.8	36.2
70-74	33.315272609916505	35.0	33.0	35.0	29.2	35.0
75-79	32.96722349595874	34.6	32.6	35.0	28.8	35.0
80-84	33.28064706790001	35.0	33.0	35.0	29.0	35.0
85-89	33.112454097536954	35.0	33.0	35.0	28.8	35.0
90-94	33.133502992467186	35.0	33.0	35.0	29.0	35.0
95-99	33.21146807250932	35.0	33.0	35.0	29.0	35.0
100-101	32.806331174619466	34.0	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	67.0
21	949.0
22	6701.0
23	26882.0
24	69948.0
25	138134.0
26	226863.0
27	329243.0
28	441530.0
29	565847.0
30	722982.0
31	948071.0
32	1331563.0
33	2022022.0
34	3225103.0
35	5822931.0
36	9408049.0
37	4943950.0
38	1392038.0
39	171094.0
40	993.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.114734651523264	21.684157959233982	17.77615844371324	28.424948945529515
2	30.253317248180867	22.3767809941408	22.19919935091585	25.17070240676248
3	27.686071774515725	21.54987950606766	22.78393350493704	27.98011521447958
4	30.14868334138807	20.5466817155955	20.882823104571877	28.421811838444555
5	30.73388699964834	20.393966176340573	19.750691328057215	29.121455495953875
6	29.241097088240046	20.075311929125377	19.174820238035817	31.508770744598756
7	31.596344364357336	19.38738220893668	19.529939380649697	29.48633404605629
8	29.18798804703751	19.66960930258041	20.063523898423785	31.07887875195829
9	29.71015566207767	18.971077274095272	20.5045340043327	30.814233059494363
10-14	30.27145726825981	19.630598558280436	19.786719582409145	30.31122459105061
15-19	30.18899584936769	19.64735558677867	19.781344735525213	30.38230382832843
20-24	30.190747304474	19.72557397290668	19.862755094107204	30.220923628512114
25-29	31.145312714969247	19.504765948370835	19.491790181656825	29.858131155003093
30-34	30.628441548732603	19.656322830588703	19.649300688139668	30.06593493253903
35-39	30.302366827441556	19.773821550205543	19.814677014691085	30.109134607661815
40-44	31.14452103610428	19.55008767783695	19.47376943513236	29.83162185092641
45-49	30.294684194561388	19.71510872463515	19.778773460862336	30.211433619941126
50-54	30.28210496175796	19.82831152872523	19.816630845503017	30.072952664013798
55-59	30.349746383836983	19.79530294823868	19.72013801534672	30.13481265257762
60-64	30.219969534096226	19.737151339214705	19.784372361306893	30.258506765382183
65-69	30.1534607227186	19.868407496638877	19.86620560949621	30.111926171146315
70-74	30.3122891807346	19.833750016194333	19.746138182354002	30.107822620717066
75-79	30.18028233229199	19.79776308148699	19.828365565169328	30.193589021051693
80-84	30.096010893893627	19.893060014575404	19.912914196316862	30.098014895214103
85-89	30.217068637777427	19.877637522823893	19.816210482186033	30.089083357212644
90-94	30.065236150522473	19.840806997470988	19.907426767066134	30.186530084940404
95-99	29.94134716192562	19.955041483107216	20.034490562099947	30.069120792867217
100-101	30.57770892944595	19.631528123823212	19.63674897797918	30.15401396875166
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.53968359705277
2	55.42401965494334
3	55.66618698899531
4	58.570495179832626
5	59.855342495602216
6	60.74986783283881
7	61.08267841041363
8	60.2668667989958
9	60.52438872157203
10-14	60.58268185931042
15-19	60.57129967769611
20-24	60.41167093298612
25-29	61.003443869972344
30-34	60.694376481271625
35-39	60.411501435103375
40-44	60.97614288703069
45-49	60.50611781450252
50-54	60.35505762577176
55-59	60.484559036414595
60-64	60.4784762994784
65-69	60.265386893864914
70-74	60.42011180145166
75-79	60.37387135334368
80-84	60.19402578910773
85-89	60.306151994990074
90-94	60.25176623546288
95-99	60.01046795479284
100-101	60.7317228981976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	518.0
1	397.5
2	270.5
3	254.5
4	257.0
5	306.0
6	338.0
7	386.0
8	480.5
9	572.5
10	730.0
11	927.5
12	1143.0
13	1455.5
14	1838.5
15	2276.0
16	2897.5
17	3623.0
18	4491.5
19	5518.0
20	6845.0
21	8440.5
22	10437.0
23	12930.0
24	15906.0
25	20057.0
26	24677.5
27	29938.0
28	36889.5
29	45073.5
30	54343.0
31	65523.5
32	78607.5
33	93025.0
34	108916.0
35	125593.0
36	142355.5
37	160279.5
38	178257.0
39	195207.0
40	211816.0
41	227458.0
42	241936.0
43	256721.5
44	273213.5
45	292016.0
46	312798.5
47	337178.0
48	367573.0
49	404739.5
50	448396.5
51	498935.0
52	557306.0
53	623273.5
54	695761.0
55	773276.5
56	854772.0
57	939630.5
58	1029037.5
59	1122630.5
60	1215972.5
61	1307953.5
62	1392394.5
63	1466291.5
64	1524434.0
65	1555491.0
66	1558190.5
67	1527972.5
68	1462619.0
69	1366256.0
70	1243211.5
71	1099398.0
72	943954.5
73	787304.0
74	636173.5
75	496177.0
76	373511.0
77	270179.0
78	186816.0
79	123193.5
80	77443.0
81	46517.0
82	26528.0
83	14813.5
84	8545.0
85	4997.0
86	2969.0
87	1882.0
88	1253.5
89	806.0
90	523.0
91	369.0
92	232.0
93	124.5
94	88.0
95	60.0
96	25.0
97	11.0
98	7.5
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.1451522683011145E-5
3	3.145152268301114E-6
4	2.8306370414710026E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.40321317562156E-5
15-19	6.290304536602229E-7
20-24	0.001574463225511538
25-29	0.20942751215027364
30-34	0.2595534153952033
35-39	0.01866780231810659
40-44	0.3244663197423179
45-49	0.0
50-54	6.800416695533018E-4
55-59	0.1013346348948156
60-64	0.012507252165817379
65-69	0.011974605776353842
70-74	6.676748128343791E-5
75-79	0.0
80-84	4.111585157814714E-4
85-89	0.0019513824377523508
90-94	0.006988750903910286
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-31	21095.0
32-33	21662.0
34-35	22194.0
36-37	24175.0
38-39	25675.0
40-41	25211.0
42-43	26505.0
44-45	21839.0
46-47	24099.0
48-49	26133.0
50-51	28296.0
52-53	30258.0
54-55	30829.0
56-57	31680.0
58-59	33972.0
60-61	35259.0
62-63	36402.0
64-65	39660.0
66-67	43339.0
68-69	45920.0
70-71	47328.0
72-73	49828.0
74-75	56189.0
76-77	37896.0
78-79	53777.0
80-81	64455.0
82-83	76381.0
84-85	85304.0
86-87	105665.0
88-89	111519.0
90-91	138606.0
92-93	180713.0
94-95	244133.0
96-97	387788.0
98-99	549188.0
100-101	2.901199E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	3.1107378692220364
#Duplication Level	Relative count
1	100.0
2	3.081113988835347
3	0.06810231857439146
4	0.012382239740798448
5	0.009802606461465437
6	0.003611486591066214
7	5.15926655866602E-4
8	0.0
9	0.0
10++	0.009802606461465437
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3180655	3.4312499	3.7258663	95-97
AAAAA	3108230	3.3571012	3.6536024	95-97
>>END_MODULE