##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	64685	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0050243487671	33.0	31.0	34.0	30.0	34.0
2	32.202535363685556	34.0	31.0	34.0	30.0	34.0
3	32.21703640720414	34.0	31.0	34.0	30.0	34.0
4	35.73383319162093	37.0	35.0	37.0	33.0	37.0
5	35.717693437427535	37.0	35.0	37.0	33.0	37.0
6	35.67638556079462	37.0	35.0	37.0	33.0	37.0
7	35.655669784339494	37.0	35.0	37.0	33.0	37.0
8	35.61062070031692	37.0	35.0	37.0	33.0	37.0
9	37.348396073278195	39.0	37.0	39.0	34.0	39.0
10-14	37.50301615521373	39.2	37.2	39.4	34.0	39.4
15-19	38.55216819973718	40.0	38.0	41.0	34.0	41.0
20-24	38.22600912112545	40.0	38.0	41.0	33.4	41.0
25-29	37.71033160701863	40.0	37.6	41.0	32.0	41.0
30-34	37.071855917136894	39.4	36.0	41.0	31.2	41.0
35-39	36.17942954317075	38.2	35.0	40.2	30.4	41.0
40-44	35.37791760068022	36.6	34.8	40.0	29.6	41.0
45-49	34.50830331607018	35.0	34.0	38.8	28.0	41.0
50-54	33.21572853057123	34.8	32.6	36.2	26.0	39.6
55-59	33.26482492076988	35.0	33.0	35.2	26.6	39.2
60-64	32.941983458297905	35.0	33.0	35.0	26.6	37.4
65-69	32.67013372497488	35.0	33.0	35.0	26.6	36.0
70-74	32.298211331838914	35.0	33.0	35.0	26.0	35.0
75-79	31.966894952461935	35.0	33.0	35.0	25.2	35.0
80-84	31.562767256705577	35.0	32.8	35.0	24.0	35.0
85-89	31.201171832727834	35.0	32.0	35.0	24.0	35.0
90-94	30.76335471902296	34.8	31.6	35.0	21.6	35.0
95-99	30.04471824998068	34.0	31.0	35.0	14.4	35.0
100-101	28.413465254695833	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	170.0
3	45.0
4	44.0
5	63.0
6	61.0
7	113.0
8	107.0
9	154.0
10	125.0
11	136.0
12	122.0
13	111.0
14	122.0
15	128.0
16	145.0
17	160.0
18	158.0
19	182.0
20	216.0
21	300.0
22	338.0
23	372.0
24	475.0
25	586.0
26	698.0
27	788.0
28	1021.0
29	1237.0
30	1465.0
31	1811.0
32	2388.0
33	3236.0
34	5106.0
35	10960.0
36	18718.0
37	9599.0
38	2859.0
39	362.0
40	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.63441109616366	22.53869586058666	17.68335111564689	27.143541927602787
2	30.46281835191514	23.301736535279655	21.490977129691196	24.74446798311401
3	28.97235279564572	21.55801583374567	21.254948045522017	28.214683325086593
4	31.07943036506734	20.334606404527392	19.940315123776536	28.645648106628734
5	30.73390949428735	20.446498971877368	19.60390222785671	29.21568930597857
6	29.49988405348999	19.799026049315916	18.73850197109067	31.962587926103424
7	31.83779520252401	19.31517654154874	19.281151889141494	29.565876366785755
8	29.20785187247668	19.737961545005955	20.177270406979442	30.87691617553792
9	30.317939197029474	18.76846909569119	19.616306954436453	31.297284752842884
10-14	30.28550575835921	19.621407983770208	19.674909387795495	30.418176870075087
15-19	30.263699085542168	19.630382142558936	19.78810060582445	30.31781816607445
20-24	30.157512940550767	19.743848213678334	19.882375495513323	30.21626335025757
25-29	30.1671624437999	19.8306730406496	19.6012343922968	30.4009301232537
30-34	30.33306531420089	19.747340425531913	19.531172686788718	30.388421573478475
35-39	30.18849838279767	19.583299834878385	19.866232938979213	30.361968843344734
40-44	30.277685878864517	19.59106186016455	19.656301499893328	30.474950761077597
45-49	30.333649740432804	19.67237951537151	19.649190069970288	30.344780674225397
50-54	30.094001236858382	19.870129870129873	19.807050092764378	30.22881880024737
55-59	30.438896189224707	19.753575017391977	19.60021643348535	30.20731235989797
60-64	30.317384246734175	19.669165958104664	19.530339336785964	30.4831104583752
65-69	30.180103578882278	19.78604004019479	19.753575017391977	30.28028136353096
70-74	30.278735410064154	19.723892710829404	19.728221380536446	30.269150498569992
75-79	30.21442374584525	19.7603772126459	19.707196413387955	30.318002628120894
80-84	30.181031150962355	19.882198345829792	19.76006802195254	30.17670248125531
85-89	30.45250057973255	19.76408750096622	19.66947514879802	30.11393677050321
90-94	30.198036639097165	19.78263894256783	19.637628507381926	30.381695910953084
95-99	30.345211409136585	19.790059519208473	19.73656952925717	30.128159542397775
100-101	30.508618690577414	19.843858699853133	19.604235912499036	30.04328669707042
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	59.77795302376645
2	55.207286335029146
3	57.187036120732316
4	59.72507847169607
5	59.949598800265925
6	61.46247197959342
7	61.40367156930976
8	60.0847680480146
9	61.61522394987236
10-14	60.703682628434294
15-19	60.58151725161661
20-24	60.373776290808344
25-29	60.5680925670536
30-34	60.72148688767937
35-39	60.5504672261424
40-44	60.75263663994212
45-49	60.6784304146582
50-54	60.32282003710575
55-59	60.64620854912267
60-64	60.80049470510937
65-69	60.460384942413235
70-74	60.54788590863415
75-79	60.53242637396614
80-84	60.357733632217666
85-89	60.56643735023576
90-94	60.579732550050245
95-99	60.473370951534356
100-101	60.55190538764783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	1.5
3	2.0
4	1.0
5	0.5
6	0.5
7	1.0
8	1.0
9	2.0
10	2.0
11	1.0
12	3.5
13	4.0
14	3.0
15	5.0
16	5.0
17	6.0
18	10.5
19	13.0
20	11.5
21	15.0
22	24.0
23	27.0
24	30.0
25	39.5
26	55.5
27	65.5
28	66.5
29	92.0
30	116.0
31	146.5
32	168.0
33	176.0
34	219.0
35	259.5
36	293.5
37	331.5
38	364.5
39	394.0
40	430.5
41	448.0
42	495.0
43	528.0
44	542.5
45	589.0
46	609.0
47	661.5
48	729.0
49	798.5
50	872.5
51	984.0
52	1135.5
53	1278.5
54	1391.5
55	1489.0
56	1642.5
57	1812.0
58	2039.5
59	2238.0
60	2337.5
61	2497.0
62	2709.5
63	2849.0
64	2921.0
65	3062.5
66	3153.5
67	3055.5
68	2922.0
69	2715.0
70	2464.5
71	2241.0
72	1922.5
73	1612.5
74	1287.5
75	983.5
76	765.0
77	544.5
78	361.5
79	233.0
80	159.5
81	97.0
82	43.0
83	20.0
84	15.5
85	12.0
86	5.5
87	6.5
88	5.0
89	0.5
90	0.5
91	0.5
92	0.5
93	1.0
94	0.5
95	1.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021643348535209092
2	0.024735255468810387
3	0.020097395068408443
4	0.01855144160160779
5	0.006183813867202597
6	0.0
7	0.040194790136816885
8	0.05874623173842468
9	0.07729767334003246
10-14	0.021334157841848958
15-19	0.019788204375048312
20-24	0.007111385947282987
25-29	0.008348148720723507
30-34	0.020097395068408443
35-39	0.008348148720723507
40-44	6.183813867202598E-4
45-49	6.183813867202598E-4
50-54	0.007729767334003247
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	64685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.04174074360361753
#Duplication Level	Relative count
1	100.0
2	0.01855918834482972
3	0.00154659902873581
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	6760	3.6393306	5.066779	30-34
AAAAA	6880	3.5536668	4.608861	45-49
AATTT	4395	2.327229	5.139969	8
>>END_MODULE