##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41764375	34.0	31.0	34.0	31.0	34.0
2	32.584084	34.0	31.0	34.0	31.0	34.0
3	32.6143535	34.0	31.0	34.0	31.0	34.0
4	36.01073525	37.0	37.0	37.0	35.0	37.0
5	36.0117635	37.0	37.0	37.0	35.0	37.0
6	35.97400575	37.0	37.0	37.0	35.0	37.0
7	35.97568425	37.0	37.0	37.0	35.0	37.0
8	35.95214125	37.0	37.0	37.0	35.0	37.0
9	37.6841925	39.0	38.0	39.0	35.0	39.0
10-14	37.9458432	39.4	38.2	39.4	35.2	39.4
15-19	39.02938385	41.0	39.0	41.0	36.0	41.0
20-24	38.7851673	40.8	39.0	41.0	35.0	41.0
25-29	38.424139499999995	40.0	38.0	41.0	34.4	41.0
30-34	37.77186164999999	40.0	37.0	41.0	33.2	41.0
35-39	36.8295688	38.6	35.0	40.8	32.0	41.0
40-44	35.97957555000001	37.0	35.0	40.0	31.0	41.0
45-49	35.0784679	35.2	34.8	39.2	30.6	41.0
50-54	33.791811949999996	35.0	33.2	36.8	28.2	39.8
55-59	33.903495150000005	35.0	34.0	35.4	30.0	39.4
60-64	33.5678134	35.0	34.0	35.0	30.0	37.6
65-69	33.2207491	35.0	34.0	35.0	29.0	36.2
70-74	32.89869055	35.0	33.6	35.0	29.0	35.2
75-79	32.602439849999996	35.0	33.0	35.0	27.8	35.0
80-84	32.29386805	35.0	33.0	35.0	27.0	35.0
85-89	31.987226050000004	35.0	33.0	35.0	26.6	35.0
90-94	31.59962545	35.0	33.0	35.0	24.4	35.0
95-99	31.056267049999995	35.0	32.8	35.0	23.0	35.0
100-101	29.959953625	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13642.0
3	2574.0
4	2771.0
5	2963.0
6	3715.0
7	4200.0
8	4951.0
9	5181.0
10	5538.0
11	5613.0
12	5504.0
13	5694.0
14	5503.0
15	5632.0
16	6026.0
17	6721.0
18	7404.0
19	8528.0
20	9703.0
21	11828.0
22	14285.0
23	17574.0
24	21151.0
25	25748.0
26	31206.0
27	37974.0
28	46615.0
29	57137.0
30	71440.0
31	90297.0
32	118761.0
33	164687.0
34	267669.0
35	619811.0
36	1296076.0
37	745525.0
38	217343.0
39	32689.0
40	321.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.71763050976101	22.57504472301003	17.7385554499031	26.968769317325858
2	30.364497358812688	23.264689649858173	21.35451353567842	25.01629945565072
3	28.902908409928145	21.419998088777774	21.473832515893914	28.203260985400163
4	30.934917916224464	20.476385621866726	19.889997883419973	28.69869857848884
5	30.628201017522784	20.475982770841284	19.586788310543028	29.309027901092904
6	29.701329010858718	19.708383049295723	18.42272200834978	32.16756593149579
7	31.858672843097875	19.223666642891914	19.348446569991005	29.56921394401921
8	29.182062525249542	19.636546768784534	19.957287912609427	31.2241027933565
9	30.138779155989614	18.658306271447984	19.912465690736475	31.290448881825927
10-14	30.302027838158395	19.55760661202542	19.707553342661353	30.432812207154836
15-19	30.285827387247515	19.546719588806738	19.67137500401509	30.496078019930657
20-24	30.190443767201668	19.6445612584297	19.76753841304097	30.397456561327658
25-29	30.263578599178892	19.733975365169613	19.693483220795684	30.30896281485581
30-34	30.275460939920812	19.596207838289	19.652331639478955	30.475999582311232
35-39	30.230083642726175	19.682286380894727	19.723834408278716	30.363795568100375
40-44	30.333510296585576	19.69524477318654	19.660570645222357	30.31067428500553
45-49	30.31997898019121	19.612933863616085	19.63508787792348	30.431999278269224
50-54	30.226033064010092	19.679834006749804	19.724387225689863	30.369745703550244
55-59	30.307612510792076	19.700952133366588	19.659286702425952	30.33214865341538
60-64	30.335781235046866	19.623398736379116	19.625428737800117	30.4153912907739
65-69	30.215798453176753	19.706316383264404	19.728571430000002	30.34931373355884
70-74	30.330788648854888	19.735748881086995	19.64191883886348	30.291543631194635
75-79	30.324389160590854	19.65560799035943	19.654852977184454	30.365149871865267
80-84	30.223395296074752	19.726435595075237	19.72436542552835	30.325803683321663
85-89	30.32487925990341	19.758395806716646	19.661260729008585	30.255464204371364
90-94	30.355129639233354	19.660830389494702	19.630435334783602	30.353604636488345
95-99	30.258291456683367	19.737258190848717	19.727087385320917	30.277362967147
100-101	30.481498134756773	19.78515358146325	19.591122784793196	30.14222549898678
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.686399827086866
2	55.38079681446341
3	57.10616939532831
4	59.6336164947133
5	59.93722891861568
6	61.8688949423545
7	61.42788678711708
8	60.40616531860604
9	61.42922803781554
10-14	60.734840045313234
15-19	60.78190540717817
20-24	60.58790032852933
25-29	60.572541414034696
30-34	60.75146052223205
35-39	60.59387921082655
40-44	60.6441845815911
45-49	60.75197825846044
50-54	60.59577876756034
55-59	60.639761164207464
60-64	60.75117252582077
65-69	60.56511218673559
70-74	60.62233228004953
75-79	60.68953903245612
80-84	60.549198979396415
85-89	60.58034346427477
90-94	60.708734275721696
95-99	60.535654423830366
100-101	60.62372363374355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	116.5
2	57.5
3	58.5
4	49.5
5	48.5
6	64.0
7	80.5
8	96.0
9	110.5
10	123.5
11	156.0
12	197.0
13	250.0
14	305.5
15	366.0
16	459.0
17	570.0
18	699.0
19	835.5
20	1008.5
21	1222.5
22	1444.0
23	1785.5
24	2209.5
25	2681.0
26	3241.5
27	3937.5
28	4815.5
29	5822.5
30	7071.5
31	8489.0
32	10085.5
33	11839.5
34	13659.5
35	15748.5
36	17888.5
37	19949.0
38	22144.5
39	24268.0
40	26095.0
41	27901.0
42	29754.5
43	31458.0
44	33436.0
45	35704.0
46	38110.0
47	40847.0
48	44380.5
49	48718.5
50	53621.0
51	59717.5
52	67087.0
53	74717.5
54	83039.5
55	92724.0
56	102860.0
57	113471.5
58	124608.5
59	135868.0
60	146770.5
61	157805.0
62	169087.5
63	178376.0
64	185410.5
65	189868.0
66	190270.5
67	186948.5
68	179658.0
69	168493.5
70	154187.0
71	136861.0
72	118119.0
73	99076.5
74	80247.0
75	62717.5
76	47240.5
77	34407.0
78	23990.5
79	15915.5
80	10066.5
81	6022.0
82	3456.5
83	1967.0
84	1147.5
85	763.0
86	573.0
87	425.0
88	341.5
89	284.5
90	255.0
91	223.5
92	176.5
93	161.0
94	140.5
95	115.0
96	90.0
97	61.0
98	48.0
99	38.5
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.065425
2	0.07335
3	0.06394999999999999
4	0.07465
5	0.041550000000000004
6	0.057249999999999995
7	0.10412500000000001
8	0.12195
9	0.1363
10-14	0.06451
15-19	0.06451
20-24	0.04241
25-29	0.04234
30-34	0.069135
35-39	0.031355
40-44	0.026324999999999998
45-49	0.01812
50-54	0.029670000000000002
55-59	0.025034999999999998
60-64	7.0E-5
65-69	2.0999999999999998E-4
70-74	4.4999999999999996E-5
75-79	0.001745
80-84	0.00819
85-89	7.999999999999999E-5
90-94	1.7999999999999998E-4
95-99	0.00792
100-101	0.0029875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5781819999000524
#Duplication Level	Relative count
1	100.0
2	0.5106733751187467
3	0.008042100395570814
4	0.002513156373615879
5	0.0010052625494463517
6	5.026312747231759E-4
7	5.026312747231759E-4
8	0.0010052625494463517
9	5.026312747231759E-4
10++	0.0015078938241695275
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	458455	3.9246047	4.108418	85-89
TTTTT	438715	3.818959	4.158973	95-97
>>END_MODULE