##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4985705	34.0	31.0	34.0	31.0	34.0
2	32.6679355	34.0	31.0	34.0	31.0	34.0
3	32.69426325	34.0	31.0	34.0	31.0	34.0
4	36.065611	37.0	37.0	37.0	35.0	37.0
5	36.01493825	37.0	37.0	37.0	35.0	37.0
6	36.04377575	37.0	37.0	37.0	35.0	37.0
7	36.05294	37.0	37.0	37.0	35.0	37.0
8	36.03189575	37.0	37.0	37.0	35.0	37.0
9	37.77517175	39.0	39.0	39.0	35.0	39.0
10-14	37.9832342	39.4	38.4	39.4	35.2	39.4
15-19	39.164917200000005	41.0	39.6	41.0	36.0	41.0
20-24	38.68914205	40.6	38.6	41.0	35.0	41.0
25-29	38.49310274999999	40.0	38.0	41.0	35.0	41.0
30-34	37.9260619	40.0	37.2	41.0	33.4	41.0
35-39	36.8699765	39.0	35.2	40.8	32.0	41.0
40-44	36.0206172	37.0	35.0	40.0	31.4	41.0
45-49	35.013322949999996	35.0	35.0	39.2	30.2	41.0
50-54	33.88879884999999	35.0	33.4	36.8	28.8	39.8
55-59	34.00689015	35.0	35.0	35.4	30.2	39.4
60-64	33.655773599999996	35.0	34.4	35.0	30.0	37.8
65-69	33.305931300000005	35.0	34.0	35.0	29.6	36.2
70-74	32.9755425	35.0	34.0	35.0	29.0	35.4
75-79	32.5964795	35.0	33.0	35.0	27.4	35.0
80-84	32.3408353	35.0	33.0	35.0	27.0	35.0
85-89	32.041267749999996	35.0	33.0	35.0	27.0	35.0
90-94	31.651079400000004	35.0	33.0	35.0	24.6	35.0
95-99	31.07470545	35.0	33.0	35.0	23.0	35.0
100-101	29.938008375	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15571.0
3	2742.0
4	2952.0
5	3203.0
6	3684.0
7	4169.0
8	4652.0
9	5057.0
10	5313.0
11	5471.0
12	5448.0
13	5362.0
14	5337.0
15	5471.0
16	5863.0
17	6418.0
18	7171.0
19	8134.0
20	9559.0
21	11288.0
22	13442.0
23	16178.0
24	19907.0
25	23781.0
26	29070.0
27	35573.0
28	43501.0
29	53225.0
30	67034.0
31	85702.0
32	114945.0
33	162335.0
34	270041.0
35	638427.0
36	1299433.0
37	751615.0
38	219472.0
39	33166.0
40	288.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.68603495967039	22.594444589102693	17.74562803824052	26.973892412986395
2	30.341026848563008	23.25179644294158	21.422467848547235	24.984708859948178
3	28.879454725612884	21.411297530132938	21.453631098624946	28.255616645629228
4	30.940976376880286	20.484270073367348	19.90340036619829	28.67135318355408
5	30.707076067645833	20.445049461204928	19.61298174828093	29.234892722868306
6	29.640383190854674	19.684177861418647	18.47109051232257	32.204348435404114
7	31.782135124237605	19.267897049868488	19.335974504993057	29.613993320900857
8	29.22389370516763	19.664428824501968	19.9398196040181	31.171857866312298
9	30.12510406560045	18.63728834778254	19.925435823604897	31.312171763012113
10-14	30.303421068683832	19.61087319669555	19.70467287530968	30.38103285931094
15-19	30.283879470940295	19.54468100650041	19.692162428714894	30.4792770938444
20-24	30.249705249795294	19.626011415417295	19.76469708963688	30.359586245150528
25-29	30.255901608411772	19.72960161579522	19.72667524801092	30.287821527782082
30-34	30.277541663176237	19.629145112347683	19.670935144932	30.42237807954408
35-39	30.248977372473107	19.739501014887264	19.727236637117617	30.284284975522013
40-44	30.326895547884313	19.730505397909617	19.653565673329123	30.289033380876944
45-49	30.312755221714372	19.60754433512196	19.68054305929471	30.399157383868964
50-54	30.207428859628717	19.684785844219448	19.740092803847766	30.367692492304073
55-59	30.29339299168735	19.72472170075128	19.687691998107848	30.294193309453522
60-64	30.293429234743385	19.64299071439257	19.653925723140578	30.409654327723462
65-69	30.212626829571587	19.729935581400152	19.730830582787405	30.326607006240856
70-74	30.30854076225965	19.75044678787977	19.675741724380465	30.265270725480114
75-79	30.349544829804188	19.67867080222597	19.628074668872582	30.34370969909726
80-84	30.204184075059338	19.744681519239172	19.75521294150775	30.295921464193736
85-89	30.328270328270328	19.755524755524757	19.672989672989676	30.243215243215243
90-94	30.336282357520012	19.670100158755403	19.639135079794453	30.354482403930128
95-99	30.244873666742304	19.734329068435553	19.75242603919567	30.268371225626474
100-101	30.514704319725976	19.81392238119347	19.584478738897534	30.086894560183016
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.65992737265678
2	55.32573570851119
3	57.13507137124212
4	59.61232956043436
5	59.941968790514146
6	61.844731626258785
7	61.39612844513845
8	60.39575157147993
9	61.437275828612556
10-14	60.684453927994774
15-19	60.763156564784694
20-24	60.60929149494583
25-29	60.54372313619386
30-34	60.69991974272032
35-39	60.53326234799512
40-44	60.61592892876126
45-49	60.71191260558333
50-54	60.57512135193278
55-59	60.587586301140874
60-64	60.70308356246685
65-69	60.539233835812446
70-74	60.57381148773976
75-79	60.693254528901456
80-84	60.50010553925308
85-89	60.571485571485574
90-94	60.69076476145015
95-99	60.513244892368775
100-101	60.60159887990899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	124.0
2	63.0
3	59.5
4	74.5
5	74.5
6	73.5
7	92.0
8	111.0
9	118.5
10	145.5
11	184.0
12	223.0
13	261.0
14	302.5
15	397.0
16	508.0
17	591.5
18	724.5
19	890.5
20	1063.5
21	1300.0
22	1542.0
23	1817.0
24	2211.5
25	2774.5
26	3344.5
27	3952.5
28	4716.0
29	5793.0
30	7171.5
31	8599.0
32	10162.5
33	11932.0
34	13867.0
35	15898.5
36	17932.5
37	20080.0
38	22327.5
39	24457.0
40	26274.0
41	28050.0
42	29941.5
43	31785.0
44	33672.5
45	35611.5
46	37941.0
47	40889.0
48	44618.0
49	48984.5
50	53843.5
51	60134.5
52	67306.5
53	74971.5
54	83620.0
55	92873.0
56	102735.5
57	113280.0
58	124279.0
59	135632.5
60	146913.0
61	158274.5
62	169600.0
63	179020.0
64	185857.0
65	189710.5
66	189934.0
67	186533.0
68	178962.0
69	167411.0
70	152992.0
71	136172.0
72	117519.5
73	98098.0
74	79288.5
75	62205.0
76	47100.5
77	34298.0
78	23889.5
79	15860.5
80	10107.5
81	6178.5
82	3688.5
83	2140.0
84	1310.5
85	872.5
86	623.0
87	498.0
88	416.5
89	388.5
90	319.5
91	260.0
92	214.5
93	180.0
94	155.5
95	127.0
96	112.5
97	86.5
98	73.0
99	61.5
100	41.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1478
2	0.146425
3	0.13835
4	0.149725
5	0.131325
6	0.10200000000000001
7	0.11377500000000002
8	0.1419
9	0.153125
10-14	0.079615
15-19	0.07554999999999999
20-24	0.050960000000000005
25-29	0.04674
30-34	0.08383
35-39	0.035695
40-44	0.032135
45-49	0.02565
50-54	0.039705
55-59	0.039705
60-64	7.999999999999999E-5
65-69	1.55E-4
70-74	8.5E-5
75-79	0.00224
80-84	0.013505
85-89	9.999999999999999E-5
90-94	2.55E-4
95-99	0.01089
100-101	0.008125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.6292794750011177
#Duplication Level	Relative count
1	100.0
2	0.5010830832943826
3	0.005528499414481653
4	0.002512954279309842
5	0.002010363423447874
6	0.0015077725675859052
7	0.001005181711723937
8	5.025908558619685E-4
9	5.025908558619685E-4
10++	0.0035181359910337793
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	461680	3.9452062	4.1728845	60-64
TTTTT	449380	3.9083054	4.1524515	95-97
>>END_MODULE