##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002003_Wally_GTTTCG_L003_R2_002.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5125625 34.0 31.0 34.0 31.0 34.0 2 32.671296 34.0 31.0 34.0 31.0 34.0 3 32.71317925 34.0 31.0 34.0 31.0 34.0 4 36.1054255 37.0 37.0 37.0 35.0 37.0 5 36.112196 37.0 37.0 37.0 35.0 37.0 6 36.08968475 37.0 37.0 37.0 35.0 37.0 7 36.07978925 37.0 37.0 37.0 35.0 37.0 8 36.0423985 37.0 37.0 37.0 35.0 37.0 9 37.78613125 39.0 39.0 39.0 35.0 39.0 10-14 38.0664432 39.4 38.4 39.4 35.2 39.4 15-19 39.21692645 41.0 39.6 41.0 36.0 41.0 20-24 39.01518374999999 41.0 39.0 41.0 35.6 41.0 25-29 38.6286433 40.2 38.4 41.0 35.0 41.0 30-34 37.983516050000006 40.0 37.4 41.0 33.6 41.0 35-39 37.056005600000006 39.0 35.2 40.8 32.8 41.0 40-44 36.1877747 37.2 35.0 40.0 31.8 41.0 45-49 35.28860945 35.2 35.0 39.2 31.0 41.0 50-54 33.9766487 35.0 33.4 36.8 29.0 39.8 55-59 34.1554124 35.0 35.0 35.4 31.0 39.4 60-64 33.760882249999995 35.0 34.8 35.0 30.6 37.8 65-69 33.40000855 35.0 34.0 35.0 30.0 36.2 70-74 33.10118715 35.0 34.0 35.0 29.0 35.4 75-79 32.8374422 35.0 33.6 35.0 29.0 35.0 80-84 32.5259373 35.0 33.0 35.0 28.2 35.0 85-89 32.213421249999996 35.0 33.0 35.0 27.0 35.0 90-94 31.8439488 35.0 33.0 35.0 26.2 35.0 95-99 31.336622800000004 35.0 33.0 35.0 24.2 35.0 100-101 30.23214025 34.0 31.5 35.0 20.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13289.0 3 2344.0 4 2570.0 5 2766.0 6 3140.0 7 3650.0 8 4257.0 9 4497.0 10 4800.0 11 4884.0 12 4817.0 13 4786.0 14 4807.0 15 4916.0 16 5306.0 17 5847.0 18 6611.0 19 7626.0 20 8796.0 21 10644.0 22 12546.0 23 15422.0 24 18721.0 25 22797.0 26 27562.0 27 33788.0 28 41979.0 29 51823.0 30 64359.0 31 82677.0 32 109243.0 33 154353.0 34 252924.0 35 613423.0 36 1343405.0 37 780673.0 38 228363.0 39 35239.0 40 350.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.69377631498915 22.56346447618826 17.718164886114497 27.02459432270809 2 30.3429301931435 23.23748486491167 21.373547744790503 25.04603719715433 3 28.910815566984922 21.388663104387852 21.437212333305972 28.263308995321257 4 30.987681908116897 20.44294784422757 19.893266725337355 28.676103522318176 5 30.679364456630037 20.40733043661455 19.563285365498487 29.35001974125693 6 29.6983792972657 19.657282772381834 18.438957953360514 32.205379976991956 7 31.89158463815221 19.20020633374483 19.347169045931796 29.56103998217116 8 29.202532057785426 19.62567729038755 19.937099355963536 31.234691295863488 9 30.162236494068612 18.62688862616223 19.91431047611414 31.29656440365502 10-14 30.30741089463646 19.56295112896857 19.680235837034182 30.44940213936079 15-19 30.288794738519343 19.526149217129873 19.669129892991474 30.515926151359313 20-24 30.207789790348777 19.60017253267763 19.75968019406924 30.432357482904354 25-29 30.288304715752535 19.696921858560497 19.678548270373355 30.336225155313613 30-34 30.302285412704915 19.576329316622598 19.649778141901887 30.471607128770593 35-39 30.232135160529094 19.659401735189512 19.729362113658045 30.379100990623346 40-44 30.327521353290788 19.662447755566845 19.646728523661945 30.363302367480422 45-49 30.333075389745524 19.58291077124499 19.615476795357157 30.468537043652326 50-54 30.230379206085527 19.656776046042317 19.716712075251113 30.39613267262104 55-59 30.310614923379042 19.683338772588773 19.65093338473901 30.355112919293177 60-64 30.34291717527925 19.614444619058446 19.608644523066857 30.433993682595446 65-69 30.237368606815874 19.70220386599174 19.70274886623699 30.357678660955393 70-74 30.311659093497727 19.752010925603276 19.64559589367877 30.29073408722023 75-79 30.313361966944775 19.63974298060703 19.631391635622915 30.415503416825278 80-84 30.233875884224975 19.723623098326424 19.716275761505948 30.326225255942653 85-89 30.33336946856015 19.74962988060574 19.654559898938704 30.262440751895397 90-94 30.33989653140294 19.661423501729892 19.597346893407025 30.401333073460147 95-99 30.27124075176937 19.724575371526 19.696256053054256 30.30792782365037 100-101 30.48652131967586 19.7928747866332 19.54777490871427 30.172828984976675 >>END_MODULE >>Per base GC content pass #Base %GC 1 59.71837063769725 2 55.38896739029783 3 57.17412456230618 4 59.66378543043507 5 60.02938419788696 6 61.90375927425765 7 61.45262462032337 8 60.43722335364892 9 61.458800897723634 10-14 60.75681303399725 15-19 60.80472088987865 20-24 60.640147273253135 25-29 60.624529871066144 30-34 60.77389254147552 35-39 60.61123615115245 40-44 60.69082372077121 45-49 60.80161243339786 50-54 60.626511878706566 55-59 60.66572784267221 60-64 60.7769108578747 65-69 60.59504726777127 70-74 60.602393180717954 75-79 60.72886538377006 80-84 60.56010114016763 85-89 60.59581022045555 90-94 60.74122960486308 95-99 60.57916857541974 100-101 60.659350304652534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 78.0 1 58.0 2 34.5 3 38.0 4 42.0 5 44.5 6 54.5 7 61.5 8 86.5 9 123.5 10 158.5 11 211.5 12 257.0 13 308.5 14 388.0 15 456.5 16 548.5 17 619.5 18 737.0 19 900.0 20 1059.0 21 1279.0 22 1501.5 23 1801.5 24 2179.0 25 2715.0 26 3350.0 27 4013.5 28 4851.5 29 5904.0 30 7075.0 31 8305.0 32 9781.0 33 11732.5 34 13754.0 35 15673.0 36 17669.0 37 19744.5 38 21853.5 39 23896.0 40 25909.5 41 27777.5 42 29612.5 43 31374.5 44 33210.5 45 35417.0 46 37767.5 47 40666.0 48 44283.5 49 48410.5 50 53449.5 51 59576.0 52 66845.5 53 74952.0 54 83667.0 55 92880.5 56 102705.0 57 113439.0 58 124450.5 59 135628.5 60 146929.0 61 158105.5 62 168887.0 63 178508.0 64 185738.0 65 189954.0 66 190653.0 67 187740.0 68 180109.5 69 168338.5 70 154222.5 71 137426.5 72 118636.0 73 98888.0 74 79892.0 75 62477.5 76 47488.5 77 34914.5 78 24035.5 79 15734.5 80 9947.0 81 5963.0 82 3441.5 83 1935.0 84 1118.0 85 723.0 86 537.5 87 416.5 88 327.5 89 288.5 90 250.0 91 214.0 92 178.0 93 131.0 94 111.0 95 105.0 96 90.5 97 74.5 98 57.5 99 42.5 100 30.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12227500000000001 2 0.10794999999999999 3 0.10139999999999999 4 0.092075 5 0.08235 6 0.143625 7 0.16175 8 0.19975 9 0.192 10-14 0.12764499999999998 15-19 0.101885 20-24 0.07690000000000001 25-29 0.07395499999999999 30-34 0.10732000000000001 35-39 0.07201 40-44 0.058730000000000004 45-49 0.049205 50-54 0.068455 55-59 0.062915 60-64 0.001655 65-69 4.4999999999999996E-5 70-74 2.9999999999999997E-5 75-79 0.016105 80-84 0.031805 85-89 0.00524 90-94 0.00251 95-99 0.032905000000000004 100-101 0.02545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.6234731651754235 #Duplication Level Relative count 1 100.0 2 0.5268423142855706 3 0.0030162727917112824 4 0.0015081363958556412 5 0.0010054242639037608 6 0.0015081363958556412 7 5.027121319518804E-4 8 0.0015081363958556412 9 0.0010054242639037608 10++ 0.005529833451470684 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 433160 3.7899673 4.024012 95-97 AAAAA 427990 3.6874413 4.009258 60-64 >>END_MODULE