##FastQC 0.10.0 >>Basic Statistics pass #Measure Value Filename 002003_Wally_GTTTCG_L003_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000000 Filtered Sequences 0 Sequence length 101 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53224225 34.0 31.0 34.0 31.0 34.0 2 32.702656 34.0 31.0 34.0 31.0 34.0 3 32.72945475 34.0 31.0 34.0 31.0 34.0 4 36.112517 37.0 37.0 37.0 35.0 37.0 5 36.11140425 37.0 37.0 37.0 35.0 37.0 6 36.08676 37.0 37.0 37.0 35.0 37.0 7 36.08994225 37.0 37.0 37.0 35.0 37.0 8 36.0647955 37.0 37.0 37.0 35.0 37.0 9 37.8184975 39.0 39.0 39.0 35.0 39.0 10-14 38.09958935 39.4 39.2 39.4 35.4 39.4 15-19 39.252437650000005 41.0 40.0 41.0 36.4 41.0 20-24 39.0486714 41.0 39.0 41.0 35.8 41.0 25-29 38.665399099999995 40.4 38.4 41.0 35.0 41.0 30-34 38.0258452 40.0 37.4 41.0 33.8 41.0 35-39 37.0933532 39.0 35.2 41.0 32.8 41.0 40-44 36.19358645 37.2 35.0 40.0 31.8 41.0 45-49 35.277642 35.2 35.0 39.4 31.0 41.0 50-54 33.944138249999995 34.8 33.4 36.8 28.8 39.8 55-59 34.149596 35.0 35.0 35.4 31.0 39.6 60-64 33.759120949999996 35.0 34.8 35.0 30.6 38.0 65-69 33.38533520000001 35.0 34.0 35.0 30.0 36.2 70-74 33.07381415 35.0 34.0 35.0 29.0 35.4 75-79 32.765590599999996 35.0 33.4 35.0 29.0 35.0 80-84 32.44039165 35.0 33.0 35.0 27.4 35.0 85-89 32.122483300000006 35.0 33.0 35.0 27.0 35.0 90-94 31.739955849999994 35.0 33.0 35.0 25.6 35.0 95-99 31.190265349999997 35.0 33.0 35.0 23.8 35.0 100-101 30.117557249999997 34.0 31.5 35.0 18.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14031.0 3 2489.0 4 2803.0 5 2842.0 6 3256.0 7 3723.0 8 4223.0 9 4536.0 10 4706.0 11 4859.0 12 5049.0 13 4929.0 14 5010.0 15 5021.0 16 5343.0 17 6015.0 18 6714.0 19 7715.0 20 9062.0 21 10641.0 22 13138.0 23 15450.0 24 18974.0 25 23243.0 26 27932.0 27 33995.0 28 41957.0 29 51857.0 30 64693.0 31 83177.0 32 110046.0 33 156263.0 34 254691.0 35 605697.0 36 1324557.0 37 790778.0 38 233525.0 39 36689.0 40 371.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.678406186726235 22.647364868841354 17.73632535408264 26.93790359034977 2 30.35585575097633 23.255589796126316 21.386345047313824 25.002209405583525 3 28.892015335722395 21.405374011633217 21.443350327129533 28.259260325514852 4 30.946161347748514 20.530892244598814 19.863465970871943 28.659480436780733 5 30.633259907758653 20.452389660737797 19.626358204150137 29.287992227353417 6 29.61269169020444 19.689389058022496 18.4363673790646 32.261551872708466 7 31.841932837009672 19.23437016287311 19.365639455025804 29.558057545091415 8 29.202897225733782 19.63254416986798 19.954858904645885 31.209699699752353 9 30.095499128957382 18.67740268014654 19.92513741836793 31.301960772528147 10-14 30.27757122437697 19.57731239139676 19.709740197290998 30.435376186935276 15-19 30.26594087898432 19.52975868286871 19.708366429230296 30.495934008916674 20-24 30.173200693557316 19.618932755732498 19.789109781168587 30.4187567695416 25-29 30.25074210609841 19.718774158597874 19.714911784782927 30.31557195052079 30-34 30.277333145676096 19.60983977278125 19.660461181352723 30.45236590018994 35-39 30.202326960167504 19.680169306239666 19.745800489807355 30.371703243785475 40-44 30.310098366967075 19.695504422101322 19.661287388862174 30.333109822069424 45-49 30.3151413608836 19.601433585328433 19.646889047104555 30.436536006683408 50-54 30.198960275979093 19.684505991019474 19.737892898020604 30.37864083498083 55-59 30.29095211805004 19.719190136704785 19.678450995997437 30.311406749247737 60-64 30.324575035548808 19.628882387655942 19.638822404056967 30.407720172738284 65-69 30.220785 19.722975 19.728485000000003 30.327755 70-74 30.314709251767404 19.73474578779663 19.66020572816458 30.290339232271386 75-79 30.297694514955637 19.659730405837415 19.645163668025596 30.397411411181352 80-84 30.22256908232141 19.73260240636528 19.722209273875148 30.322619237438165 85-89 30.334342455903368 19.769605652193064 19.64127490145817 30.254776990445393 90-94 30.352877999462997 19.66712717058095 19.637681920296323 30.34231290965973 95-99 30.252324803643628 19.74689000092123 19.71397680882772 30.28680838660743 100-101 30.50039592914331 19.784580541389037 19.568726023283684 30.146297506183974 >>END_MODULE >>Per base GC content pass #Base %GC 1 59.616309777076005 2 55.35806515655985 3 57.15127566123724 4 59.605641784529254 5 59.92125213511207 6 61.8742435629129 7 61.39999038210109 8 60.41259692548613 9 61.39745990148553 10-14 60.71294741131224 15-19 60.76187488790099 20-24 60.59195746309891 25-29 60.5663140566192 30-34 60.72969904586603 35-39 60.57403020395298 40-44 60.6432081890365 45-49 60.75167736756701 50-54 60.577601110959925 55-59 60.60235886729778 60-64 60.73229520828709 65-69 60.548539999999996 70-74 60.60504848403879 75-79 60.69510592613698 80-84 60.54518831975958 85-89 60.58911944634876 90-94 60.69519090912273 95-99 60.53913319025105 100-101 60.64669343532728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 85.0 1 61.0 2 37.5 3 38.0 4 38.0 5 36.0 6 46.0 7 60.0 8 83.5 9 107.0 10 135.0 11 180.0 12 225.0 13 275.0 14 343.5 15 429.5 16 535.5 17 652.0 18 783.0 19 925.5 20 1089.5 21 1260.5 22 1507.0 23 1818.0 24 2178.5 25 2686.0 26 3258.0 27 3961.5 28 4814.5 29 5745.0 30 6955.5 31 8425.5 32 10054.5 33 11905.0 34 13933.0 35 15800.0 36 17790.0 37 20117.5 38 22353.0 39 24386.5 40 26159.5 41 28000.0 42 30012.0 43 31885.0 44 33695.5 45 35815.0 46 37985.0 47 40838.0 48 44681.5 49 48969.5 50 53981.5 51 59858.5 52 66878.5 53 75103.0 54 83722.5 55 92966.0 56 103010.0 57 113374.0 58 124445.0 59 135459.0 60 146686.0 61 158523.0 62 169601.0 63 178573.0 64 185227.5 65 189257.0 66 189936.0 67 187001.0 68 179944.5 69 168621.5 70 153635.0 71 136136.0 72 117381.0 73 98106.0 74 79166.0 75 61871.0 76 46868.5 77 34031.0 78 23688.5 79 15961.5 80 10204.5 81 6198.0 82 3623.0 83 2087.0 84 1271.0 85 869.0 86 653.5 87 515.0 88 412.5 89 355.0 90 292.0 91 232.5 92 214.0 93 200.5 94 180.5 95 154.0 96 128.0 97 99.5 98 73.0 99 56.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.173375 2 0.142825 3 0.135125 4 0.1238 5 0.136975 6 0.169325 7 0.18610000000000002 8 0.190725 9 0.1771 10-14 0.12671500000000002 15-19 0.08832 20-24 0.071705 25-29 0.06146 30-34 0.101555 35-39 0.06275 40-44 0.04978 45-49 0.045015 50-54 0.0504 55-59 0.07153 60-64 1.65E-4 65-69 0.0 70-74 7.999999999999999E-5 75-79 0.01193 80-84 0.03014 85-89 5.85E-4 90-94 8.5E-4 95-99 0.02489 100-101 0.013675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 0.6428956780626206 #Duplication Level Relative count 1 100.0 2 0.5309173910638961 3 0.009049728256770957 4 5.027626809317198E-4 5 0.0030165760855903187 6 0.0010055253618634396 7 0.0015082880427951593 8 0.0010055253618634396 9 5.027626809317198E-4 10++ 0.005027626809317198 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 447435 3.8897552 4.2182393 90-94 AAAAA 440705 3.7747397 4.1003876 90-94 >>END_MODULE