##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R1_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.644221	34.0	31.0	34.0	31.0	34.0
2	32.8938945	34.0	31.0	34.0	31.0	34.0
3	32.938315	34.0	31.0	34.0	31.0	34.0
4	36.369516	37.0	37.0	37.0	35.0	37.0
5	36.28872925	37.0	37.0	37.0	35.0	37.0
6	36.25646575	37.0	37.0	37.0	35.0	37.0
7	36.248622	37.0	37.0	37.0	35.0	37.0
8	36.23267675	37.0	37.0	37.0	35.0	37.0
9	38.00417475	39.0	38.0	39.0	35.0	39.0
10-14	38.2640309	39.4	38.2	39.4	35.2	39.4
15-19	39.382115899999995	41.0	39.0	41.0	36.0	41.0
20-24	38.962660899999996	40.4	38.8	41.0	35.2	41.0
25-29	34.84062290000001	38.8	29.2	41.0	23.8	41.0
30-34	35.761803799999996	38.8	34.8	40.2	26.0	41.0
35-39	36.3677774	38.0	35.0	40.0	30.6	41.0
40-44	34.9356811	36.0	34.4	39.6	27.6	41.0
45-49	34.9584616	35.0	34.6	38.8	29.4	41.0
50-54	33.7500783	35.0	33.6	36.8	26.8	40.2
55-59	33.7682648	35.0	33.6	35.0	29.2	39.0
60-64	32.808677	35.0	33.0	35.0	26.0	37.2
65-69	32.95440975	35.0	33.0	35.0	28.6	36.0
70-74	32.0229801	35.0	33.0	35.0	24.4	35.0
75-79	32.01790875	34.4	32.4	35.0	26.6	35.0
80-84	31.799041800000005	35.0	33.0	35.0	25.0	35.0
85-89	31.513822450000003	35.0	32.6	35.0	24.2	35.0
90-94	31.324262749999995	35.0	32.8	35.0	24.0	35.0
95-99	30.881434050000003	35.0	32.0	35.0	23.0	35.0
100-101	29.416724375	33.5	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92.0
3	16.0
4	61.0
5	141.0
6	478.0
7	1481.0
8	3804.0
9	5144.0
10	5338.0
11	5968.0
12	6260.0
13	6303.0
14	6491.0
15	7067.0
16	7652.0
17	8272.0
18	8632.0
19	9765.0
20	10813.0
21	12348.0
22	14914.0
23	18693.0
24	24042.0
25	30177.0
26	38368.0
27	48319.0
28	60019.0
29	75522.0
30	97396.0
31	130418.0
32	187994.0
33	299828.0
34	488957.0
35	804705.0
36	987078.0
37	457829.0
38	117952.0
39	11595.0
40	68.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.10216199417604	21.72730016123762	17.809918626132497	28.36061921845384
2	30.224600000000002	22.391875	22.260225	25.123299999999997
3	27.661974999999998	21.5558	22.817275000000002	27.964949999999998
4	30.145625	20.573475	20.930375	28.350524999999998
5	30.742725	20.3842	19.740725	29.132350000000002
6	29.264250000000004	20.0612	19.165925	31.508625000000002
7	31.658125	19.33645	19.518774999999998	29.486649999999997
8	29.213050000000003	19.646325	20.036375	31.10425
9	29.717875	18.9224	20.543074999999998	30.816650000000003
10-14	30.28959	19.6109	19.768069999999998	30.331439999999997
15-19	30.213245	19.620325	19.76321	30.403219999999997
20-24	30.26190511667089	19.708599532334432	19.796750581331917	30.232744769662762
25-29	33.68394241703272	18.79992242360811	18.69739575972403	28.81873939963514
30-34	31.192829429001655	19.595559621707746	19.429859750271877	29.781751199018718
35-39	30.446494238860648	19.688209514163713	19.75405467924552	30.11124156773012
40-44	30.533854133797306	19.632077555015297	19.638948881872047	30.195119429315355
45-49	30.431745	19.67927	19.690985	30.198000000000004
50-54	30.5101739997052	19.795633997213173	19.706403122750604	29.987788880331028
55-59	30.376563079457092	19.691129884239707	19.67415976799441	30.25814726830879
60-64	30.40513539939634	19.65581120855433	19.649034361863606	30.29001903018573
65-69	30.291313982082922	19.762592672535803	19.765133221802554	30.180960123578714
70-74	30.53543	19.753165	19.59795	30.113455000000002
75-79	30.35192	19.648145	19.69407	30.305864999999997
80-84	30.325678844057936	19.753325437488613	19.746105274673944	30.174890443779507
85-89	30.47177290826094	19.77635701584385	19.608104944085504	30.143765131809708
90-94	30.342412072671326	19.65092382027926	19.67464454053095	30.332019566518465
95-99	30.333761341465486	19.72690481119141	19.73431995207909	30.20501389526401
100-101	30.766225000000002	19.5749875	19.4423875	30.2164
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.46278121262989
2	55.3479
3	55.62692500000001
4	58.49615
5	59.875075
6	60.772875000000006
7	61.144774999999996
8	60.317299999999996
9	60.534524999999995
10-14	60.62103
15-19	60.616465
20-24	60.494649886333654
25-29	62.50268181666786
30-34	60.974580628020384
35-39	60.55773580659077
40-44	60.72897356311265
45-49	60.629745
50-54	60.497962880036226
55-59	60.63471034776589
60-64	60.69515442958207
65-69	60.47227410566164
70-74	60.648885
75-79	60.657785
80-84	60.500569287837436
85-89	60.615538040070646
90-94	60.674431639189784
95-99	60.538775236729506
100-101	60.982625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	42.5
2	29.0
3	27.5
4	31.0
5	28.5
6	27.0
7	40.0
8	54.5
9	62.0
10	86.0
11	108.5
12	131.5
13	176.5
14	211.0
15	258.0
16	339.5
17	424.5
18	522.5
19	638.5
20	825.0
21	1040.5
22	1226.0
23	1511.5
24	1875.0
25	2362.0
26	2990.5
27	3595.5
28	4415.0
29	5399.5
30	6445.5
31	7809.5
32	9444.0
33	11317.0
34	13281.5
35	15236.0
36	17308.0
37	19675.0
38	21921.5
39	23863.5
40	26034.0
41	28091.0
42	29782.0
43	31676.5
44	33692.0
45	35716.5
46	38057.5
47	41319.5
48	45185.5
49	49282.5
50	54649.5
51	61089.0
52	67983.0
53	75907.5
54	84915.0
55	94337.5
56	104187.0
57	114382.0
58	124862.5
59	136127.0
60	148016.0
61	159410.5
62	169591.5
63	178871.5
64	185932.0
65	189755.5
66	190733.0
67	187541.5
68	179756.0
69	168228.5
70	153646.0
71	136444.0
72	117236.5
73	97902.5
74	79526.5
75	62026.5
76	46671.5
77	33982.5
78	23514.0
79	15559.0
80	9812.5
81	5818.0
82	3253.0
83	1799.5
84	1035.5
85	594.5
86	372.0
87	260.0
88	181.0
89	142.0
90	106.0
91	66.0
92	54.0
93	37.0
94	18.5
95	12.5
96	5.5
97	2.5
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007825
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0011899999999999999
25-29	0.021130000000000003
30-34	0.069325
35-39	0.030625
40-44	0.9652700000000001
45-49	0.0
50-54	9.8E-4
55-59	6.85E-4
60-64	0.02725
65-69	0.02162
70-74	0.0
75-79	0.0
80-84	0.002255
85-89	0.01609
90-94	0.024115
95-99	0.0019000000000000002
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.15347928029716712
#Duplication Level	Relative count
1	100.0
2	0.1366913679150811
3	0.001001401962747847
4	0.001001401962747847
5	0.0
6	0.0
7	0.0
8	5.007009813739235E-4
9	0.0
10++	0.001001401962747847
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	419930	3.6542354	4.315714	95-97
AAAAA	412110	3.571741	4.0733533	95-97
>>END_MODULE