##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7627195	34.0	31.0	34.0	31.0	34.0
2	33.00077725	34.0	31.0	34.0	31.0	34.0
3	33.033694	34.0	31.0	34.0	31.0	34.0
4	36.43258425	37.0	37.0	37.0	35.0	37.0
5	36.367651	37.0	37.0	37.0	35.0	37.0
6	36.332247	37.0	37.0	37.0	35.0	37.0
7	36.329147	37.0	37.0	37.0	35.0	37.0
8	36.30697	37.0	37.0	37.0	35.0	37.0
9	38.08698025	39.0	38.0	39.0	37.0	39.0
10-14	38.35332375	39.4	38.4	39.4	36.2	39.4
15-19	39.484551950000004	41.0	39.6	41.0	37.0	41.0
20-24	39.245903549999994	40.6	39.0	41.0	35.8	41.0
25-29	38.827196550000004	40.0	38.2	41.0	35.0	41.0
30-34	38.1051022	40.0	37.2	41.0	33.6	41.0
35-39	37.130701	38.6	35.2	40.6	32.6	41.0
40-44	36.23169475	37.0	35.0	40.0	31.8	41.0
45-49	35.7211394	35.2	35.0	39.4	32.0	41.0
50-54	34.89114059999999	35.0	35.0	37.6	31.2	40.6
55-59	34.2481631	35.0	34.2	35.4	31.0	39.4
60-64	33.722344250000006	35.0	34.0	35.0	30.0	37.6
65-69	33.292787249999996	35.0	33.2	35.0	29.2	36.2
70-74	32.964021949999996	35.0	33.0	35.0	29.0	35.2
75-79	32.28663815	34.4	32.6	35.0	27.6	35.0
80-84	32.507491849999994	35.0	33.0	35.0	28.2	35.0
85-89	32.2125174	35.0	33.0	35.0	27.0	35.0
90-94	31.7875545	35.0	33.0	35.0	26.2	35.0
95-99	31.25282085	35.0	33.0	35.0	24.2	35.0
100-101	29.924609625000002	34.0	30.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61.0
3	19.0
4	45.0
5	123.0
6	304.0
7	847.0
8	2021.0
9	3141.0
10	4013.0
11	4388.0
12	4667.0
13	4757.0
14	5197.0
15	5542.0
16	6182.0
17	6977.0
18	7565.0
19	8244.0
20	8797.0
21	9890.0
22	11739.0
23	14403.0
24	18244.0
25	23349.0
26	29382.0
27	36195.0
28	45460.0
29	56831.0
30	71741.0
31	91642.0
32	120544.0
33	167760.0
34	275563.0
35	669525.0
36	1345583.0
37	717042.0
38	199746.0
39	22325.0
40	146.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.096672933143886	21.66015058047214	17.789802902248205	28.453373584135772
2	30.288925	22.38685	22.164875000000002	25.159350000000003
3	27.641650000000002	21.551175	22.79575	28.011425000000003
4	30.176625	20.51465	20.85795	28.450775
5	30.800975	20.3296	19.732425	29.137
6	29.25505	20.050625	19.139875	31.55445
7	31.645450000000004	19.35265	19.50555	29.49635
8	29.239475	19.623575	20.000899999999998	31.136049999999997
9	29.79135	18.924625	20.44385	30.840175
10-14	30.294105	19.603105000000003	19.747895	30.354895
15-19	30.239054999999997	19.600965000000002	19.73585	30.424129999999998
20-24	30.15899603514925	19.714687347998055	19.85192910469254	30.274387512160157
25-29	30.316693845948844	19.68944368013564	19.716328794397377	30.277533679518136
30-34	30.24370471910715	19.625158393711192	19.76556930396406	30.3655675832176
35-39	30.216778107579827	19.71839852914696	19.799900901036498	30.26492246223671
40-44	30.355178288149563	19.65808431596188	19.675744454593968	30.31099294129459
45-49	30.26512	19.616715000000003	19.74279	30.375375
50-54	30.21829479885405	19.722487348799724	19.795162781218547	30.26405507112767
55-59	30.37314333957251	19.649854212171977	19.661604238609538	30.31539820964597
60-64	30.28449728332163	19.625612053597354	19.717175927039825	30.372714736041186
65-69	30.24008386755049	19.72452450630032	19.772665320557802	30.262726305591386
70-74	30.39544	19.65801	19.65529	30.291259999999998
75-79	30.316405000000003	19.652	19.6947	30.336895000000002
80-84	30.25534658715739	19.734110124731824	19.75346157125245	30.257081716858337
85-89	30.420988235449464	19.670896290902192	19.633753344728305	30.274362128920036
90-94	30.35078296674232	19.640350578375486	19.668558160367432	30.340308294514767
95-99	30.334873342997774	19.691939727098752	19.72646368009137	30.246723249812103
100-101	30.75804119929074	19.571394374551165	19.42404244055703	30.24652198560106
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.550046517279654
2	55.448275
3	55.653074999999994
4	58.627399999999994
5	59.937975
6	60.80950000000001
7	61.1418
8	60.375524999999996
9	60.631524999999996
10-14	60.649
15-19	60.663185
20-24	60.433383547309404
25-29	60.594227525466984
30-34	60.60927230232475
35-39	60.481700569816546
40-44	60.66617122944415
45-49	60.64049500000001
50-54	60.482349869981725
55-59	60.68854154921849
60-64	60.65721201936282
65-69	60.502810173141874
70-74	60.6867
75-79	60.6533
80-84	60.51242830401573
85-89	60.6953503643695
90-94	60.69109126125708
95-99	60.58159659280987
100-101	61.0045631848918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	53.0
2	23.0
3	23.0
4	25.0
5	34.0
6	37.5
7	45.0
8	57.0
9	66.0
10	85.5
11	115.0
12	150.5
13	190.0
14	248.5
15	308.0
16	369.0
17	452.0
18	583.0
19	719.0
20	877.5
21	1044.5
22	1277.0
23	1608.5
24	2005.5
25	2574.0
26	3189.5
27	3766.0
28	4643.5
29	5781.0
30	6911.0
31	8332.0
32	9989.5
33	11660.0
34	13677.0
35	15794.0
36	17806.0
37	20030.0
38	22226.0
39	24352.5
40	26220.5
41	28017.5
42	29915.0
43	31593.0
44	33532.5
45	35926.0
46	38546.5
47	41300.0
48	45038.0
49	49877.0
50	55125.0
51	61048.0
52	68007.0
53	76116.5
54	85107.5
55	94438.0
56	104328.5
57	114728.0
58	125375.0
59	136269.0
60	147505.5
61	159040.5
62	169488.0
63	178769.0
64	185959.0
65	189039.5
66	189656.0
67	186701.5
68	178663.0
69	167351.5
70	152726.0
71	135275.0
72	116476.5
73	97778.5
74	79373.0
75	61961.5
76	46740.5
77	33914.0
78	23519.0
79	15389.5
80	9585.0
81	5716.0
82	3230.0
83	1756.0
84	950.0
85	581.5
86	397.0
87	242.5
88	162.0
89	123.0
90	86.0
91	58.5
92	40.0
93	26.0
94	19.0
95	10.5
96	4.0
97	4.0
98	2.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010275000000000001
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0012799999999999999
25-29	4.25E-4
30-34	0.043379999999999995
35-39	0.009045000000000001
40-44	7.849999999999999E-4
45-49	0.0
50-54	5.949999999999999E-4
55-59	2.25E-4
60-64	0.042455
65-69	0.032850000000000004
70-74	0.0
75-79	0.0
80-84	0.007475
85-89	0.034855
90-94	0.044605
95-99	0.01145
100-101	0.0013125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5518813455107157
#Duplication Level	Relative count
1	100.0
2	0.5212653124827208
3	0.0035186665259200056
4	5.026666465600008E-4
5	0.0010053332931200015
6	0.002513333232800004
7	0.0015079999396800023
8	0.0010053332931200015
9	5.026666465600008E-4
10++	0.0015079999396800023
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	414985	3.5352123	3.8290699	9
AAAAA	407505	3.5093913	3.8493652	95-97
>>END_MODULE