##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78265375	34.0	31.0	34.0	31.0	34.0
2	33.01285075	34.0	31.0	34.0	31.0	34.0
3	33.056008	34.0	31.0	34.0	31.0	34.0
4	36.43539625	37.0	37.0	37.0	35.0	37.0
5	36.373006	37.0	37.0	37.0	35.0	37.0
6	36.33383575	37.0	37.0	37.0	35.0	37.0
7	36.3286255	37.0	37.0	37.0	35.0	37.0
8	36.3058115	37.0	37.0	37.0	35.0	37.0
9	38.09052775	39.0	39.0	39.0	37.0	39.0
10-14	38.36951985	39.4	38.6	39.4	36.6	39.4
15-19	39.49650335	41.0	39.6	41.0	37.0	41.0
20-24	39.2699411	40.8	39.0	41.0	36.0	41.0
25-29	38.83549925	40.0	38.2	41.0	35.0	41.0
30-34	38.112122299999996	40.0	37.2	41.0	33.6	41.0
35-39	37.1481082	38.8	35.2	40.6	32.6	41.0
40-44	36.2282802	37.2	35.0	40.0	31.8	41.0
45-49	35.700574700000004	35.2	35.0	39.4	32.0	41.0
50-54	34.858211849999996	35.0	35.0	37.6	31.0	40.6
55-59	34.1963223	35.0	34.2	35.4	30.8	39.4
60-64	33.636697149999996	35.0	34.0	35.0	30.0	37.6
65-69	33.19395975	35.0	33.0	35.0	29.0	36.2
70-74	32.85073835	35.0	33.0	35.0	29.0	35.2
75-79	32.165904049999995	34.4	32.6	35.0	26.8	35.0
80-84	32.376397850000004	35.0	33.0	35.0	27.0	35.0
85-89	32.047396400000004	35.0	33.0	35.0	27.0	35.0
90-94	31.59491775	35.0	33.0	35.0	25.0	35.0
95-99	30.98900335	35.0	32.4	35.0	23.0	35.0
100-101	29.735809	34.0	30.5	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68.0
3	19.0
4	38.0
5	110.0
6	343.0
7	911.0
8	2107.0
9	3358.0
10	4407.0
11	4842.0
12	5061.0
13	5361.0
14	5623.0
15	6168.0
16	6865.0
17	7663.0
18	8453.0
19	9082.0
20	9609.0
21	10939.0
22	12817.0
23	15794.0
24	19552.0
25	24409.0
26	30547.0
27	37980.0
28	47355.0
29	57994.0
30	73107.0
31	93254.0
32	123217.0
33	171367.0
34	277383.0
35	647973.0
36	1307769.0
37	732126.0
38	211035.0
39	25153.0
40	141.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.11763205664822	21.687644708482733	17.75862500062509	28.436098234243968
2	30.288375000000002	22.364375	22.154925	25.192325
3	27.669349999999998	21.544025	22.765575	28.02105
4	30.139875	20.536775	20.86085	28.462500000000002
5	30.737150000000003	20.399800000000003	19.719225	29.143825
6	29.301225	20.064899999999998	19.1433	31.490575
7	31.602175	19.422475	19.4756	29.499750000000002
8	29.180650000000004	19.672025	20.01295	31.134375
9	29.715075000000002	18.96835	20.467675	30.8489
10-14	30.263	19.6061	19.791719999999998	30.33918
15-19	30.209855	19.637845000000002	19.773815	30.378484999999998
20-24	30.150324894093668	19.703358262314104	19.866925323259075	30.279391520333153
25-29	30.29835633489146	19.71952627508659	19.739096395442832	30.24302099457912
30-34	30.205859792543865	19.655719891448896	19.793267248235935	30.345153067771303
35-39	30.192609750352922	19.759764129690666	19.826107654833645	30.22151846512277
40-44	30.34298362760523	19.69190939404544	19.68560429978428	30.279502678565045
45-49	30.263370000000002	19.623255	19.76078	30.352594999999997
50-54	30.203006116533643	19.72989351441433	19.80756394160168	30.25953642745035
55-59	30.3752530375253	19.683536968353696	19.6710419671042	30.2701680270168
60-64	30.27256875012879	19.65295782053034	19.734758555041417	30.339714874299457
65-69	30.240119839846745	19.739763471354223	19.788574701088113	30.231541987710912
70-74	30.402649999999998	19.6949	19.646365	30.256085
75-79	30.299545	19.654445000000003	19.714154999999998	30.331855
80-84	30.27952771930379	19.734388361975725	19.767680948809723	30.218402969910763
85-89	30.413400820622122	19.67850681622884	19.645868777479894	30.26222358566915
90-94	30.366902287950598	19.633888085342708	19.677603732242737	30.321605894463953
95-99	30.341377152724657	19.718646088498364	19.73267243011794	30.207304328659035
100-101	30.77243848639827	19.563976620818814	19.436675634236163	30.22690925854675
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.55373029089218
2	55.480700000000006
3	55.6904
4	58.602374999999995
5	59.88097500000001
6	60.791799999999995
7	61.101925
8	60.315025
9	60.563975000000006
10-14	60.602180000000004
15-19	60.588339999999995
20-24	60.42971641442683
25-29	60.54137732947058
30-34	60.55101286031517
35-39	60.41412821547569
40-44	60.62248630617027
45-49	60.615965
50-54	60.462542543983986
55-59	60.645421064542106
60-64	60.612283624428244
65-69	60.47166182755767
70-74	60.658735
75-79	60.6314
80-84	60.49793068921455
85-89	60.67562440629127
90-94	60.68850818241456
95-99	60.548681481383696
100-101	60.99934774494502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	45.0
2	21.5
3	23.5
4	23.5
5	27.0
6	35.5
7	38.5
8	56.0
9	66.5
10	75.5
11	109.0
12	135.0
13	186.0
14	244.0
15	265.0
16	353.0
17	472.5
18	571.5
19	695.0
20	878.5
21	1082.0
22	1364.0
23	1695.0
24	2011.5
25	2507.0
26	3109.0
27	3823.5
28	4742.0
29	5749.5
30	6978.5
31	8451.5
32	10027.0
33	11848.5
34	13841.0
35	15888.5
36	17925.5
37	20086.0
38	22188.5
39	24294.0
40	26650.0
41	28660.0
42	30281.5
43	31863.5
44	33880.5
45	36434.5
46	38948.5
47	41714.5
48	45188.5
49	49844.0
50	55331.5
51	61265.0
52	68270.0
53	76524.5
54	85319.5
55	94675.5
56	104670.0
57	114761.0
58	125668.5
59	136992.5
60	147934.5
61	158469.0
62	168776.5
63	178331.5
64	185406.5
65	189550.0
66	189565.0
67	185165.5
68	177846.5
69	166652.5
70	151841.0
71	134860.5
72	115565.0
73	96404.0
74	78648.5
75	61714.5
76	46442.5
77	33677.5
78	23713.5
79	15754.0
80	9824.0
81	5984.5
82	3541.0
83	2032.5
84	1174.5
85	696.0
86	459.5
87	319.5
88	228.5
89	150.0
90	113.5
91	90.0
92	58.0
93	39.0
94	25.0
95	13.5
96	7.0
97	6.5
98	5.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0145
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.00126
25-29	6.150000000000001E-4
30-34	0.052605
35-39	0.01285
40-44	0.001495
45-49	0.0
50-54	5.5E-4
55-59	9.999999999999999E-6
60-64	0.03146
65-69	0.03325
70-74	0.0
75-79	0.0
80-84	0.007770000000000001
85-89	0.024630000000000003
90-94	0.04723
95-99	0.009564999999999999
100-101	7.750000000000001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5458526197926608
#Duplication Level	Relative count
1	100.0
2	0.5051215810054182
3	0.006533910998080036
4	0.002513042691569244
5	5.026085383138489E-4
6	5.026085383138489E-4
7	5.026085383138489E-4
8	5.026085383138489E-4
9	0.0030156512298830933
10++	0.002513042691569244
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	420105	3.571137	3.9725976	95-97
AAAAA	414205	3.543419	3.8565867	80-84
>>END_MODULE