##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R2_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	124585	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.129662479431715	33.0	31.0	34.0	30.0	34.0
2	32.30404944415459	34.0	31.0	34.0	30.0	34.0
3	32.34842075691295	34.0	31.0	34.0	30.0	34.0
4	35.72995946542521	37.0	35.0	37.0	35.0	37.0
5	35.81070754906289	37.0	35.0	37.0	35.0	37.0
6	35.778103302965846	37.0	36.0	37.0	35.0	37.0
7	35.69724284624955	37.0	35.0	37.0	35.0	37.0
8	35.701697636152026	37.0	35.0	37.0	33.0	37.0
9	37.442637556688204	39.0	38.0	39.0	35.0	39.0
10-14	37.59953445438857	39.2	37.4	39.4	34.4	39.4
15-19	38.654712846650874	40.0	38.0	41.0	34.4	41.0
20-24	38.379230244411445	40.0	38.0	41.0	33.6	41.0
25-29	37.99181763454669	40.0	37.8	41.0	33.0	41.0
30-34	37.27741381386202	39.6	36.2	41.0	31.8	41.0
35-39	36.256833487177424	38.4	35.0	40.2	30.6	41.0
40-44	35.35657583176145	36.6	35.0	40.0	29.4	41.0
45-49	34.62038768712124	35.0	34.0	38.8	28.8	41.0
50-54	33.334277802303646	34.8	32.6	36.2	26.2	39.8
55-59	33.45653810651363	35.0	33.6	35.2	27.0	39.2
60-64	33.07174539471043	35.0	33.0	35.0	27.0	37.6
65-69	32.72036440984067	35.0	33.0	35.0	26.4	36.2
70-74	32.36377894610106	35.0	33.0	35.0	26.0	35.0
75-79	32.04377413011197	35.0	33.0	35.0	25.8	35.0
80-84	31.71459003892925	35.0	33.0	35.0	24.6	35.0
85-89	31.379594654252116	35.0	32.6	35.0	24.0	35.0
90-94	30.913084239675726	35.0	31.8	35.0	22.4	35.0
95-99	30.32009150379259	34.6	31.0	35.0	16.8	35.0
100-101	28.847959224625757	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	303.0
3	71.0
4	85.0
5	111.0
6	159.0
7	188.0
8	216.0
9	214.0
10	240.0
11	251.0
12	235.0
13	226.0
14	238.0
15	265.0
16	261.0
17	311.0
18	309.0
19	338.0
20	410.0
21	464.0
22	568.0
23	746.0
24	891.0
25	985.0
26	1232.0
27	1568.0
28	1844.0
29	2169.0
30	2714.0
31	3426.0
32	4403.0
33	5985.0
34	9087.0
35	20710.0
36	37500.0
37	19350.0
38	5738.0
39	765.0
40	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.7614582413436	22.437199444448904	17.767198400783553	27.034143913423947
2	30.46060975805545	23.207892497631928	21.253231011286463	25.078266733026155
3	28.920982282627023	21.55386257997704	21.23676415101913	28.28839098637681
4	31.028780154939188	20.438325372295587	19.92534018383976	28.607554288925463
5	30.925393741872302	20.25061409282836	19.760945301587814	29.063046863711527
6	29.825082481757686	19.586908880736594	18.569834554839332	32.01817408266639
7	31.82102778981843	19.331663696639858	19.22570598339996	29.62160253014176
8	29.294705070160227	19.68018495328003	19.88247760331375	31.142632373245995
9	30.217958495564563	18.695460201501223	19.965479869947416	31.12110143298679
10-14	30.345228386301315	19.604522132247645	19.63839638332167	30.41185309812937
15-19	30.24349364495554	19.622534279673328	19.760600169210985	30.373371906160145
20-24	30.285989485090504	19.627402977886582	19.691937231608943	30.394670305413975
25-29	30.21374964883413	19.784083156078182	19.701087610868083	30.301079584219607
30-34	30.366611496481898	19.620144448493466	19.687247660618283	30.325996394406353
35-39	30.165365542771738	19.806460830632357	19.778206937294016	30.24996668930189
40-44	30.36208211261388	19.706224665890755	19.596741180719988	30.334952040775377
45-49	30.210538989444956	19.699803347112415	19.66817835212907	30.42147931131356
50-54	30.226752819360275	19.696432154753783	19.63687442308464	30.4399406028013
55-59	30.29080547417426	19.819079343420153	19.558534333988842	30.331580848416746
60-64	30.304129710639323	19.71521451218044	19.55885539992776	30.421800377252477
65-69	30.17104787895814	19.793554601276238	19.733996869607097	30.301400650158527
70-74	30.336075771561582	19.831921980976844	19.63382429666493	30.198177950796644
75-79	30.372677288598144	19.74282618292732	19.575711361720913	30.308785166753623
80-84	30.206846731147408	19.89806156439379	19.769795721796363	30.125295982662436
85-89	30.295300397319096	19.839467030541396	19.719227836416906	30.1460047357226
90-94	30.246498374603686	19.698198017417827	19.691776698639483	30.363526909339004
95-99	30.174900670225146	19.752779227033752	19.768511458040695	30.303808644700403
100-101	30.456314965686076	19.817795079664485	19.76321387004856	29.962676084600876
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.79560215476754
2	55.5388764910816
3	57.20937326900383
4	59.63633444386465
5	59.988440605583826
6	61.843256564424074
7	61.44263031996019
8	60.43733744340622
9	61.33905992855135
10-14	60.757081484430685
15-19	60.61686555111569
20-24	60.68065979050448
25-29	60.51482923305373
30-34	60.69260789088825
35-39	60.415332232073624
40-44	60.697034153389254
45-49	60.63201830075852
50-54	60.66669342216158
55-59	60.622386322591005
60-64	60.7259300878918
65-69	60.47244852911666
70-74	60.53425372235823
75-79	60.68146245535176
80-84	60.332142713809844
85-89	60.4413051330417
90-94	60.61002528394269
95-99	60.47870931492555
100-101	60.418991050286955
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	3.5
2	1.0
3	1.5
4	1.5
5	1.0
6	1.0
7	1.5
8	1.5
9	1.5
10	3.5
11	5.0
12	9.0
13	11.0
14	5.5
15	7.0
16	16.0
17	19.5
18	20.0
19	24.0
20	28.5
21	31.0
22	38.5
23	55.0
24	75.5
25	90.0
26	109.5
27	138.5
28	153.0
29	171.0
30	211.0
31	246.5
32	287.5
33	370.5
34	428.5
35	506.0
36	585.5
37	623.0
38	692.0
39	790.0
40	841.0
41	871.5
42	895.5
43	979.5
44	1053.0
45	1071.5
46	1152.0
47	1236.5
48	1342.0
49	1484.5
50	1665.0
51	1839.0
52	2088.5
53	2374.5
54	2610.5
55	2881.0
56	3207.5
57	3569.5
58	3900.0
59	4218.0
60	4582.0
61	4984.0
62	5345.0
63	5689.5
64	5843.5
65	5852.5
66	5878.0
67	5878.0
68	5760.5
69	5317.0
70	4762.5
71	4281.0
72	3657.0
73	3030.0
74	2458.0
75	1874.0
76	1424.5
77	1062.5
78	742.5
79	462.0
80	279.0
81	154.5
82	80.5
83	49.0
84	23.5
85	14.5
86	12.5
87	8.0
88	7.0
89	5.5
90	3.0
91	2.5
92	2.5
93	1.5
94	1.0
95	2.5
96	2.5
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019263956335032306
2	0.008829313320223141
3	0.014447967251274231
4	0.016053296945860256
5	0.008829313320223141
6	0.009631978167516153
7	0.00561865393105109
8	0.010434643014809166
9	0.016053296945860256
10-14	0.005297587992133884
15-19	0.0054581209615924865
20-24	0.0
25-29	0.0
30-34	6.421318778344102E-4
35-39	3.210659389172051E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	124585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.08989846289681744
#Duplication Level	Relative count
1	100.0
2	0.044989676476022915
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	14050	3.790204	6.019276	9
TTTTT	13520	3.7796066	4.7461133	8
>>END_MODULE