##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R2_004.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.487379	34.0	31.0	34.0	31.0	34.0
2	32.660209	34.0	31.0	34.0	31.0	34.0
3	32.69530675	34.0	31.0	34.0	31.0	34.0
4	36.0573615	37.0	37.0	37.0	35.0	37.0
5	36.0699425	37.0	37.0	37.0	35.0	37.0
6	36.0619855	37.0	37.0	37.0	35.0	37.0
7	36.040506	37.0	37.0	37.0	35.0	37.0
8	36.02456825	37.0	37.0	37.0	35.0	37.0
9	37.76164625	39.0	38.0	39.0	35.0	39.0
10-14	38.0009649	39.4	38.2	39.4	35.2	39.4
15-19	39.11426135000001	41.0	39.0	41.0	36.0	41.0
20-24	38.93208625	41.0	39.0	41.0	35.4	41.0
25-29	38.512898899999996	40.2	38.2	41.0	34.8	41.0
30-34	37.8936255	40.0	37.2	41.0	33.4	41.0
35-39	36.92667014999999	38.8	35.2	40.8	32.4	41.0
40-44	36.024942249999995	37.0	35.0	40.0	31.2	41.0
45-49	35.13299920000001	35.2	35.0	39.2	30.8	41.0
50-54	33.8775038	35.0	33.4	36.8	28.6	39.8
55-59	34.03548454999999	35.0	35.0	35.4	30.4	39.4
60-64	33.63929460000001	35.0	34.4	35.0	30.0	37.8
65-69	33.298872599999996	35.0	34.0	35.0	29.6	36.2
70-74	32.97150755	35.0	34.0	35.0	29.0	35.4
75-79	32.6604272	35.0	33.0	35.0	28.2	35.0
80-84	32.36191315000001	35.0	33.0	35.0	27.0	35.0
85-89	32.04386555000001	35.0	33.0	35.0	27.0	35.0
90-94	31.660983150000003	35.0	33.0	35.0	24.6	35.0
95-99	31.12477505	35.0	33.0	35.0	23.6	35.0
100-101	30.048521625	34.0	31.0	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13169.0
3	2422.0
4	2758.0
5	3036.0
6	3457.0
7	4237.0
8	4926.0
9	5266.0
10	5538.0
11	5567.0
12	5639.0
13	5412.0
14	5462.0
15	5565.0
16	5770.0
17	6413.0
18	7364.0
19	8179.0
20	9800.0
21	11503.0
22	13874.0
23	16752.0
24	20252.0
25	24756.0
26	29741.0
27	36188.0
28	43869.0
29	54386.0
30	67544.0
31	85005.0
32	112426.0
33	157519.0
34	257596.0
35	618442.0
36	1317639.0
37	763015.0
38	225352.0
39	33818.0
40	343.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.733622592326725	22.547939536346576	17.763581440351654	26.95485643097505
2	30.348295300455234	23.280439562199092	21.382923683059683	24.988341454285994
3	28.88050120628529	21.430826468030688	21.432202363738796	28.256469961945225
4	30.973245894028206	20.443006690027396	19.88527093310173	28.698476482842672
5	30.66697482367266	20.425381731992452	19.668546124250305	29.239097320084582
6	29.6223660014847	19.695485660202934	18.44271684778585	32.23943149052651
7	31.830103358526703	19.230299688693602	19.360310886117496	29.579286066662196
8	29.204530498493952	19.680960466481405	19.909832410789484	31.204676624235155
9	30.123241714258693	18.67765952096105	19.907874622806688	31.291224141973572
10-14	30.281300288960107	19.594923622182222	19.698088287323998	30.425687801533673
15-19	30.28588831185164	19.559525210789623	19.66672150957841	30.487864967780332
20-24	30.169405033216023	19.61168284154725	19.787768736656968	30.431143388579763
25-29	30.301723489075776	19.713882579251745	19.694498147001312	30.289895784671167
30-34	30.303237663740724	19.620114814715883	19.64494034818974	30.431707173353654
35-39	30.213351297203232	19.69420942776075	19.7444089680576	30.34803030697842
40-44	30.322869069392684	19.699976371957888	19.65139511712133	30.325759441528096
45-49	30.342361006166147	19.62324927212506	19.606246937704547	30.42814278400424
50-54	30.21646111943278	19.692019978872057	19.718401148108487	30.373117753586676
55-59	30.318912266507684	19.724433900558417	19.650779173295703	30.305874659638192
60-64	30.318	19.637535	19.629075	30.41539
65-69	30.21816342719457	19.702493735598374	19.73051385468388	30.348828982523173
70-74	30.31427	19.748095	19.666805	30.27083
75-79	30.343186613984013	19.660283473115175	19.648423175421705	30.348106737479107
80-84	30.23797980379827	19.727808499301567	19.725418380751687	30.30879331614848
85-89	30.325940000000003	19.766365	19.6664	30.241295
90-94	30.337195	19.664125	19.630705000000003	30.367975
95-99	30.26415316983804	19.749086989043867	19.712521550258604	30.27423829085949
100-101	30.49411397053079	19.785171643306924	19.584056000875012	30.13665838528727
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.68847902330177
2	55.33663675474123
3	57.136971168230524
4	59.671722376870875
5	59.90607214375725
6	61.86179749201122
7	61.4093894251889
8	60.409207122729114
9	61.41446585623226
10-14	60.70698809049377
15-19	60.77375327963197
20-24	60.600548421795786
25-29	60.59161927374694
30-34	60.73494483709438
35-39	60.561381604181655
40-44	60.64862851092078
45-49	60.770503790170395
50-54	60.589578873019455
55-59	60.62478692614588
60-64	60.73339
65-69	60.56699240971775
70-74	60.585100000000004
75-79	60.69129335146313
80-84	60.54677311994675
85-89	60.567235
90-94	60.705169999999995
95-99	60.538391460697525
100-101	60.63077235581807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	135.0
2	76.5
3	61.5
4	61.5
5	69.5
6	71.0
7	78.0
8	85.5
9	100.0
10	135.5
11	158.5
12	201.0
13	258.5
14	305.5
15	356.5
16	424.0
17	521.5
18	634.5
19	815.0
20	1005.0
21	1197.5
22	1437.0
23	1790.5
24	2212.5
25	2689.0
26	3246.0
27	3957.5
28	4822.0
29	5799.0
30	6994.0
31	8343.0
32	9985.0
33	11932.5
34	13762.5
35	15731.0
36	17888.5
37	20037.0
38	22065.0
39	24132.0
40	26281.0
41	28062.0
42	29856.5
43	31714.5
44	33645.0
45	35688.5
46	38036.0
47	40981.5
48	44348.5
49	48551.0
50	53709.0
51	59625.0
52	66972.5
53	75282.5
54	83434.0
55	92376.0
56	102576.5
57	113229.0
58	124200.5
59	135441.0
60	146615.5
61	157978.0
62	169070.0
63	178645.5
64	185711.0
65	189720.0
66	190561.5
67	187584.0
68	179917.5
69	168453.5
70	153647.0
71	136512.5
72	118269.0
73	98844.5
74	79819.5
75	62611.5
76	47502.5
77	34483.0
78	23959.0
79	15984.0
80	10068.5
81	5982.5
82	3446.5
83	1945.5
84	1161.0
85	798.0
86	579.0
87	434.0
88	368.5
89	299.5
90	253.0
91	224.0
92	184.0
93	152.5
94	133.0
95	113.5
96	85.5
97	73.0
98	56.0
99	37.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08377499999999999
2	0.0733
3	0.0651
4	0.0602
5	0.050949999999999995
6	0.0474
7	0.0663
8	0.08605
9	0.092675
10-14	0.043295
15-19	0.047855
20-24	0.020385
25-29	0.022865
30-34	0.04243
35-39	0.028965
40-44	0.012875000000000001
45-49	0.013730000000000001
50-54	0.023385
55-59	0.019995000000000002
60-64	0.0
65-69	4.25E-4
70-74	0.0
75-79	0.00251
80-84	0.00496
85-89	0.0
90-94	0.0
95-99	0.0012000000000000001
100-101	0.0015625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5682158920539776
#Duplication Level	Relative count
1	100.0
2	0.5076320722948487
3	0.006031272146077411
4	0.0010052120243462352
5	0.0010052120243462352
6	0.0010052120243462352
7	5.026060121731176E-4
8	0.0
9	0.0
10++	0.004020848097384941
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	455340	3.902378	4.117926	65-69
TTTTT	444125	3.868047	4.2271194	95-97
>>END_MODULE