##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R2_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.464216	34.0	31.0	34.0	31.0	34.0
2	32.64421725	34.0	31.0	34.0	31.0	34.0
3	32.6778375	34.0	31.0	34.0	31.0	34.0
4	36.036727	37.0	37.0	37.0	35.0	37.0
5	36.03561275	37.0	37.0	37.0	35.0	37.0
6	36.03779475	37.0	37.0	37.0	35.0	37.0
7	36.0294035	37.0	37.0	37.0	35.0	37.0
8	36.0222235	37.0	37.0	37.0	35.0	37.0
9	37.7487875	39.0	38.0	39.0	35.0	39.0
10-14	37.99098725	39.4	38.4	39.4	35.2	39.4
15-19	39.008269150000004	41.0	39.0	41.0	35.8	41.0
20-24	38.62488385	40.6	38.6	41.0	34.6	41.0
25-29	38.483565600000006	40.0	38.0	41.0	34.8	41.0
30-34	37.856156150000004	40.0	37.2	41.0	33.4	41.0
35-39	36.84645715	38.8	35.2	40.8	32.0	41.0
40-44	35.971102849999994	37.0	35.0	40.0	31.0	41.0
45-49	35.101118150000005	35.2	35.0	39.2	30.6	41.0
50-54	33.84973715	34.8	33.4	36.8	28.6	39.8
55-59	34.010031	35.0	35.0	35.4	30.0	39.4
60-64	33.607466949999996	35.0	34.4	35.0	30.0	37.8
65-69	33.25275435	35.0	34.0	35.0	29.2	36.2
70-74	32.936882250000004	35.0	34.0	35.0	29.0	35.4
75-79	32.537502700000005	35.0	33.0	35.0	27.8	35.0
80-84	32.260154400000005	35.0	33.0	35.0	27.0	35.0
85-89	31.964919	35.0	33.0	35.0	26.6	35.0
90-94	31.408131100000002	35.0	33.0	35.0	23.4	35.0
95-99	30.9186597	35.0	32.4	35.0	22.2	35.0
100-101	29.567604875	34.0	30.5	35.0	7.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14857.0
3	2815.0
4	3087.0
5	3320.0
6	3812.0
7	4355.0
8	4832.0
9	5462.0
10	5691.0
11	5609.0
12	5769.0
13	5510.0
14	5508.0
15	5652.0
16	5952.0
17	6651.0
18	7385.0
19	8588.0
20	9790.0
21	11683.0
22	14015.0
23	17107.0
24	20584.0
25	24871.0
26	30394.0
27	36474.0
28	44760.0
29	54707.0
30	68804.0
31	87799.0
32	116061.0
33	165488.0
34	276644.0
35	650228.0
36	1287331.0
37	731830.0
38	214705.0
39	31572.0
40	298.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.72723720702369	22.5628105588312	17.774553480300185	26.935398753844925
2	30.314560399663076	23.236125119060617	21.417590022845616	25.031724458430695
3	28.899153302680276	21.404014602925393	21.46682100001602	28.230011094378316
4	30.928019587324318	20.492311774926023	19.92757396568213	28.652094672067534
5	30.681466541554048	20.46102064258082	19.597483193416167	29.260029622448968
6	29.624241314697713	19.725517736754334	18.44261803077459	32.20762291777337
7	31.792166206995752	19.228453316822705	19.383077856370036	29.596302619811503
8	29.217740946747632	19.679930341312556	19.92248469335415	31.179844018585655
9	30.117206092634756	18.6996219755668	19.900161109579038	31.28301082221941
10-14	30.277976036457165	19.606285856941927	19.709526538932668	30.40621156766824
15-19	30.401061841726158	19.548797299676664	19.635683444312836	30.41445741428434
20-24	30.22595485143888	19.686076129659234	19.726604891977644	30.36136412692424
25-29	30.26648117701446	19.728807900586933	19.701602573783948	30.30310834861466
30-34	30.281890851439762	19.62430325842667	19.666209291674992	30.42759659845857
35-39	30.265333472962	19.710201504368847	19.722854101947128	30.301610920722023
40-44	30.32388803465645	19.698238327500064	19.656065966314422	30.321807671529065
45-49	30.33545025850889	19.609127655313475	19.623663858184266	30.431758227993367
50-54	30.221033429129392	19.697477241601707	19.71902235210192	30.362466977166985
55-59	30.302996963814266	19.716143913593555	19.68322810559923	30.29763101699295
60-64	30.324629097388726	19.627240888172267	19.65004589501377	30.398084119425235
65-69	30.206827655120744	19.723539792654847	19.738314803736102	30.331317748488313
70-74	30.324485000000003	19.728815	19.660574999999998	30.286125000000002
75-79	30.341384217373868	19.668446048043297	19.648720515453917	30.34144921912892
80-84	30.216126812157274	19.73672104713554	19.737671090267497	30.30948105043969
85-89	30.335895	19.761465	19.65662	30.24602
90-94	30.360833396916735	19.70409167450084	19.62035658239224	30.31471834619018
95-99	30.277186428596288	19.74135406828903	19.69908293542267	30.282376567692015
100-101	30.530779686383312	19.84603062511196	19.57340959921534	30.049780089289385
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.66263596086861
2	55.34628485809377
3	57.12916439705859
4	59.58011425939185
5	59.94149616400301
6	61.83186423247108
7	61.388468826807255
8	60.397584965333294
9	61.40021691485417
10-14	60.684187604125405
15-19	60.8155192560105
20-24	60.58731897836312
25-29	60.56958952562912
30-34	60.70948744989833
35-39	60.56694439368402
40-44	60.645695706185506
45-49	60.767208486502255
50-54	60.58350040629637
55-59	60.60062798080721
60-64	60.722713216813965
65-69	60.53814540360906
70-74	60.61061
75-79	60.68283343650278
80-84	60.525607862596964
85-89	60.581914999999995
90-94	60.675551743106915
95-99	60.5595629962883
100-101	60.5805597756727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	177.0
1	135.5
2	86.5
3	83.0
4	75.5
5	71.5
6	86.0
7	92.0
8	103.0
9	127.0
10	155.5
11	194.0
12	221.5
13	255.0
14	309.5
15	382.0
16	453.0
17	573.5
18	713.0
19	856.5
20	1022.5
21	1202.0
22	1495.0
23	1766.5
24	2119.0
25	2657.5
26	3265.0
27	3931.5
28	4794.5
29	5859.5
30	7051.0
31	8381.0
32	10036.5
33	11943.0
34	13845.5
35	15903.0
36	18091.5
37	20158.0
38	22175.5
39	24256.0
40	26157.0
41	27994.0
42	29954.0
43	31622.5
44	33523.5
45	35706.5
46	38035.0
47	40889.0
48	44291.0
49	48535.5
50	53669.0
51	59914.0
52	66919.0
53	74683.0
54	83377.0
55	92652.5
56	102561.5
57	113047.0
58	124327.5
59	135888.5
60	147070.0
61	158238.0
62	168736.5
63	178205.5
64	185731.5
65	190094.0
66	190645.0
67	187490.5
68	180390.5
69	168571.0
70	153543.0
71	136296.5
72	117327.5
73	98252.5
74	79745.0
75	62286.0
76	47048.5
77	34322.5
78	23919.5
79	15841.0
80	10059.5
81	6099.0
82	3490.5
83	2037.0
84	1311.5
85	874.5
86	642.0
87	527.0
88	424.5
89	343.5
90	293.5
91	267.5
92	230.5
93	199.5
94	159.0
95	125.0
96	111.5
97	97.5
98	77.5
99	57.5
100	40.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.184975
2	0.1559
3	0.1296
4	0.13949999999999999
5	0.1433
6	0.0409
7	0.064375
8	0.08425
9	0.09905
10-14	0.039405
15-19	0.0416
20-24	0.02162
25-29	0.01958
30-34	0.038259999999999995
35-39	0.02053
40-44	0.017455
45-49	0.008275000000000001
50-54	0.023719999999999998
55-59	0.017645
60-64	2.9999999999999997E-5
65-69	7.500000000000001E-5
70-74	0.0
75-79	0.0026999999999999997
80-84	0.00454
85-89	0.0
90-94	1.1E-4
95-99	0.00268
100-101	0.007712500000000001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5796753817861899
#Duplication Level	Relative count
1	100.0
2	0.515199646145804
3	0.005026338011178575
4	0.0
5	0.0015079014033535726
6	0.001005267602235715
7	5.026338011178575E-4
8	0.001005267602235715
9	5.026338011178575E-4
10++	0.00201053520447143
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	463345	3.9425542	4.3135056	60-64
TTTTT	452850	3.941258	4.281911	95-97
>>END_MODULE