##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R2_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58061025	34.0	31.0	34.0	31.0	34.0
2	32.768438	34.0	31.0	34.0	31.0	34.0
3	32.77628875	34.0	31.0	34.0	31.0	34.0
4	36.15281	37.0	37.0	37.0	35.0	37.0
5	36.1548675	37.0	37.0	37.0	35.0	37.0
6	36.132018	37.0	37.0	37.0	35.0	37.0
7	36.12329225	37.0	37.0	37.0	35.0	37.0
8	36.10096875	37.0	37.0	37.0	35.0	37.0
9	37.86582475	39.0	39.0	39.0	37.0	39.0
10-14	38.15781979999999	39.4	39.2	39.4	35.8	39.4
15-19	39.314620000000005	41.0	40.0	41.0	36.8	41.0
20-24	39.116405900000004	41.0	39.2	41.0	35.8	41.0
25-29	38.7357698	40.4	38.6	41.0	35.0	41.0
30-34	38.08586205	40.0	37.4	41.0	34.0	41.0
35-39	37.15542725	39.0	35.2	41.0	33.0	41.0
40-44	36.22726485	37.0	35.0	40.0	32.0	41.0
45-49	35.37854125	35.2	35.0	39.4	31.2	41.0
50-54	34.14908815	35.0	33.8	37.0	29.6	39.8
55-59	34.292747799999994	35.0	35.0	35.4	31.0	39.6
60-64	33.88212875	35.0	35.0	35.0	31.0	37.8
65-69	33.51636995	35.0	34.4	35.0	30.4	36.2
70-74	33.209991650000006	35.0	34.0	35.0	29.8	35.4
75-79	32.9386822	35.0	34.0	35.0	29.0	35.0
80-84	32.637367700000006	35.0	33.0	35.0	29.0	35.0
85-89	32.332406500000005	35.0	33.0	35.0	27.0	35.0
90-94	31.9919545	35.0	33.0	35.0	27.0	35.0
95-99	31.4896231	35.0	33.0	35.0	24.6	35.0
100-101	30.421735875000003	34.0	31.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12085.0
3	2235.0
4	2452.0
5	2563.0
6	2935.0
7	3474.0
8	4017.0
9	4225.0
10	4547.0
11	4629.0
12	4573.0
13	4463.0
14	4466.0
15	4701.0
16	5187.0
17	5734.0
18	6250.0
19	7205.0
20	8326.0
21	9967.0
22	11825.0
23	14587.0
24	17573.0
25	21255.0
26	25680.0
27	31945.0
28	39259.0
29	48370.0
30	60901.0
31	77910.0
32	103567.0
33	147312.0
34	243175.0
35	609318.0
36	1371385.0
37	801323.0
38	234111.0
39	36102.0
40	368.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.77099076479958	22.51187136116429	17.731254315777456	26.985883558258678
2	30.360172264460832	23.257428961180434	21.354201467536246	25.028197306822484
3	28.849150803647603	21.406585398022234	21.4440254682574	28.30023833007276
4	31.03264997153528	20.45085613117544	19.855161498182635	28.661332399106648
5	30.702070477305828	20.407339008753357	19.58470339173911	29.305887122201703
6	29.64520596271712	19.671873791140655	18.415749216753035	32.26717102938919
7	31.872223547944213	19.133415180320863	19.366461247922004	29.627900023812913
8	29.269122210339933	19.563396754715278	19.960479554806444	31.207001480138345
9	30.162525241193627	18.627700407064328	19.871956032244622	31.33781831949742
10-14	30.336106519833894	19.5495657970695	19.665642895688634	30.44868478740797
15-19	30.303173146985113	19.507439652079803	19.659909196827428	30.52947800410766
20-24	30.2161277022466	19.584703968349174	19.75584345991616	30.443324869488066
25-29	30.29732851755095	19.664315759976876	19.67541748151418	30.362938240957988
30-34	30.31538445215478	19.56012392409209	19.643036596683775	30.48145502706936
35-39	30.23011222513128	19.647175230011914	19.73158184887968	30.391130695977125
40-44	30.342438347092887	19.676758006900283	19.628817138472833	30.351986507533997
45-49	30.350145721278437	19.562329477693744	19.60069863783229	30.48682616319553
50-54	30.23435820877456	19.64716146651207	19.70661795706058	30.411862367652787
55-59	30.3144091565983	19.661599843528258	19.653676210542535	30.37031478933091
60-64	30.34268009034855	19.60415958537347	19.617974669645484	30.43518565463249
65-69	30.245381880341917	19.69094052095781	19.719140564667875	30.344537034032403
70-74	30.343905	19.716205000000002	19.628105	30.311785
75-79	30.340590683339506	19.62403196052857	19.607885885199956	30.427491470931965
80-84	30.25035613819627	19.69456852182908	19.721897412096613	30.333177927878037
85-89	30.351924017900707	19.720782225376055	19.655426107786443	30.271867648936794
90-94	30.375630547186482	19.6414570459409	19.594281788835747	30.388630618036867
95-99	30.28209926925159	19.7240663923239	19.69360812415172	30.30022621427279
100-101	30.466757485455513	19.75959982392973	19.565352461202096	30.20829022941266
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.75687432305826
2	55.38836957128331
3	57.14938913372036
4	59.693982370641926
5	60.007957599507534
6	61.91237699210631
7	61.50012357175713
8	60.476123690478275
9	61.50034356069105
10-14	60.78479130724187
15-19	60.83265115109276
20-24	60.65945257173466
25-29	60.66026675850894
30-34	60.79683947922414
35-39	60.62124292110841
40-44	60.69442485462688
45-49	60.83697188447397
50-54	60.646220576427346
55-59	60.68472394592921
60-64	60.77786574498104
65-69	60.589918914374316
70-74	60.65568999999999
75-79	60.768082154271475
80-84	60.58353406607431
85-89	60.6237916668375
90-94	60.76426116522335
95-99	60.58232548352438
100-101	60.67504771486817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	57.0
2	37.5
3	37.0
4	38.5
5	47.0
6	49.5
7	55.0
8	66.5
9	84.5
10	116.0
11	159.5
12	204.0
13	242.0
14	315.0
15	392.5
16	458.0
17	567.0
18	682.5
19	817.5
20	998.0
21	1235.0
22	1487.5
23	1754.0
24	2129.5
25	2630.0
26	3228.5
27	3943.0
28	4699.0
29	5713.0
30	6942.5
31	8242.5
32	9843.0
33	11689.0
34	13601.0
35	15650.0
36	17794.0
37	19871.5
38	21913.0
39	24029.0
40	25934.5
41	27727.5
42	29499.0
43	31482.5
44	33358.0
45	35035.0
46	37450.0
47	40589.5
48	44061.0
49	48248.5
50	53442.5
51	59858.5
52	66946.5
53	74628.5
54	83105.0
55	92397.5
56	102585.0
57	113123.5
58	124176.5
59	135115.0
60	146536.5
61	158539.5
62	169667.5
63	178967.0
64	186072.5
65	190586.0
66	190892.5
67	187756.5
68	180717.5
69	169184.5
70	154412.0
71	137041.5
72	118291.0
73	99094.5
74	80393.5
75	62759.5
76	47473.0
77	34824.0
78	24182.0
79	16036.0
80	10066.5
81	5859.5
82	3410.0
83	1938.5
84	1133.0
85	735.5
86	525.0
87	403.5
88	323.0
89	271.5
90	230.5
91	206.5
92	186.0
93	153.0
94	129.5
95	105.0
96	80.5
97	72.0
98	52.0
99	33.0
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14835
2	0.101275
3	0.10702500000000001
4	0.095625
5	0.0955
6	0.151225
7	0.159225
8	0.17825
9	0.1632
10-14	0.109495
15-19	0.08168500000000001
20-24	0.049370000000000004
25-29	0.060545
30-34	0.09368
35-39	0.061155
40-44	0.033100000000000004
45-49	0.036905
50-54	0.036145
55-59	0.045849999999999995
60-64	6.1E-4
65-69	1.55E-4
70-74	0.0
75-79	0.006660000000000001
80-84	0.014235
85-89	0.00171
90-94	5.45E-4
95-99	0.01073
100-101	0.0115125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.6260523725536302
#Duplication Level	Relative count
1	100.0
2	0.5465332595907285
3	0.003519533410427875
4	0.001005580974407964
5	5.02790487203982E-4
6	0.0025139524360199106
7	5.02790487203982E-4
8	0.0
9	0.0
10++	0.003519533410427875
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	435855	3.815708	4.0798016	85-89
AAAAA	434740	3.760186	4.019624	80-84
>>END_MODULE