##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5085895	34.0	31.0	34.0	31.0	34.0
2	32.6754465	34.0	31.0	34.0	31.0	34.0
3	32.7139245	34.0	31.0	34.0	31.0	34.0
4	36.08683575	37.0	37.0	37.0	35.0	37.0
5	36.08733725	37.0	37.0	37.0	35.0	37.0
6	36.076142	37.0	37.0	37.0	35.0	37.0
7	36.0651055	37.0	37.0	37.0	35.0	37.0
8	36.04607825	37.0	37.0	37.0	35.0	37.0
9	37.80500275	39.0	39.0	39.0	35.0	39.0
10-14	38.09300234999999	39.4	38.8	39.4	35.4	39.4
15-19	39.2271726	41.0	39.6	41.0	36.0	41.0
20-24	39.01949525	41.0	39.0	41.0	35.6	41.0
25-29	38.6526201	40.4	38.4	41.0	35.0	41.0
30-34	37.99824220000001	40.0	37.4	41.0	33.6	41.0
35-39	37.0676744	39.0	35.2	41.0	32.6	41.0
40-44	36.1151826	37.0	35.0	40.0	31.6	41.0
45-49	35.251733650000006	35.2	35.0	39.2	31.0	41.0
50-54	33.99303625	34.8	33.8	37.0	29.2	39.8
55-59	34.1626841	35.0	35.0	35.4	31.0	39.4
60-64	33.7552187	35.0	34.8	35.0	30.6	38.0
65-69	33.3895028	35.0	34.0	35.0	30.0	36.2
70-74	33.073334	35.0	34.0	35.0	29.0	35.4
75-79	32.76416245	35.0	33.4	35.0	29.0	35.0
80-84	32.4488229	35.0	33.0	35.0	27.4	35.0
85-89	32.135073999999996	35.0	33.0	35.0	27.0	35.0
90-94	31.772988500000004	35.0	33.0	35.0	25.8	35.0
95-99	31.221487200000002	35.0	33.0	35.0	23.8	35.0
100-101	30.14241675	34.0	31.5	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12178.0
3	2572.0
4	2854.0
5	2890.0
6	3353.0
7	3828.0
8	4274.0
9	4669.0
10	4878.0
11	5058.0
12	5030.0
13	4900.0
14	5070.0
15	5039.0
16	5491.0
17	6043.0
18	6997.0
19	7773.0
20	9026.0
21	10830.0
22	13047.0
23	15719.0
24	19072.0
25	23200.0
26	28226.0
27	34260.0
28	42440.0
29	52623.0
30	65909.0
31	84964.0
32	111335.0
33	156393.0
34	254589.0
35	605366.0
36	1324621.0
37	787618.0
38	231547.0
39	35937.0
40	381.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.67898452801657	22.592382707494384	17.78616705773772	26.942465706751324
2	30.339585450244062	23.247823911755557	21.390492969561056	25.02209766843932
3	28.929626166001277	21.3802350343087	21.450091827881874	28.24004697180815
4	30.936267119189264	20.520644674455625	19.89851790169851	28.644570304656604
5	30.68301483743715	20.38709231598919	19.608616339257594	29.321276507316067
6	29.614316382093865	19.681146319623487	18.473507525625568	32.23102977265709
7	31.84617429444863	19.23016560172833	19.38302912066306	29.540630983159982
8	29.178879426356026	19.666559425554848	19.96021646847925	31.194344679609877
9	30.091022210741087	18.6799664276905	19.904159216652786	31.32485214491563
10-14	30.309548840274488	19.57641322306689	19.70162153875236	30.412416397906263
15-19	30.268826112169663	19.5375679804314	19.690997948130565	30.502607959268367
20-24	30.193707802275966	19.589516464683072	19.785456840953806	30.431318892087155
25-29	30.272870605896358	19.702188749736226	19.710506465886436	30.31443417848098
30-34	30.282609808271317	19.603709989484237	19.671005067067693	30.442675135176756
35-39	30.202947471945365	19.676165496674123	19.740478867788582	30.38040816359193
40-44	30.32336247605405	19.68649945984397	19.6480819184806	30.342056145621388
45-49	30.332467505192344	19.598238741046377	19.636302176271407	30.432991577489872
50-54	30.214098525719386	19.678538902847887	19.712165189282135	30.39519738215059
55-59	30.31304103514071	19.705740396752063	19.678104611200357	30.30311395690688
60-64	30.334598200758922	19.620851772511063	19.632506779504066	30.412043247225952
65-69	30.217125	19.71578	19.723975	30.343120000000003
70-74	30.31272	19.743305	19.64788	30.296095
75-79	30.331395633725773	19.648634026778456	19.62788367402246	30.39208666547331
80-84	30.21472503837742	19.742111147284437	19.729490762993734	30.313673051344413
85-89	30.354125354125355	19.741619741619743	19.664299664299666	30.23995523995524
90-94	30.355505	19.6719	19.60774	30.364855000000002
95-99	30.26124589611777	19.743826844583776	19.70934020819288	30.285587051105573
100-101	30.460572304609094	19.79430115494966	19.585004655200176	30.160121885241065
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	59.62145023476789
2	55.36168311868338
3	57.169673137809426
4	59.580837423845864
5	60.00429134475321
6	61.84534615475095
7	61.386805277608616
8	60.3732241059659
9	61.41587435565672
10-14	60.72196523818075
15-19	60.77143407143804
20-24	60.625026694363115
25-29	60.58730478437734
30-34	60.72528494344807
35-39	60.583355635537295
40-44	60.665418621675435
45-49	60.76545908268221
50-54	60.60929590786998
55-59	60.61615499204759
60-64	60.74664144798487
65-69	60.560245
70-74	60.60881500000001
75-79	60.723482299199084
80-84	60.52839808972183
85-89	60.59408059408059
90-94	60.72035999999999
95-99	60.54683294722335
100-101	60.62069418985017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	59.0
2	37.0
3	44.5
4	43.5
5	45.0
6	51.5
7	62.0
8	74.0
9	85.5
10	105.0
11	136.0
12	179.5
13	223.0
14	274.5
15	342.0
16	428.0
17	519.5
18	639.0
19	804.5
20	954.5
21	1170.5
22	1465.0
23	1793.0
24	2182.0
25	2685.5
26	3295.5
27	3928.0
28	4775.5
29	5866.5
30	7071.5
31	8429.0
32	9953.5
33	11795.5
34	13736.0
35	15673.0
36	17797.5
37	20011.5
38	22283.0
39	24322.0
40	26274.0
41	28283.5
42	30048.0
43	31780.5
44	33715.0
45	35674.0
46	38092.0
47	41001.5
48	44486.0
49	48769.5
50	53778.5
51	59908.0
52	66727.5
53	74507.5
54	83326.5
55	92659.5
56	102794.5
57	113225.5
58	124184.0
59	135733.0
60	146984.5
61	158444.0
62	169421.5
63	178471.5
64	185795.5
65	189973.0
66	190378.5
67	187021.0
68	179300.0
69	168482.5
70	154010.0
71	136301.0
72	117336.5
73	98374.5
74	79994.0
75	62691.0
76	47351.5
77	34281.5
78	23858.5
79	15859.0
80	9969.0
81	6064.5
82	3524.5
83	2032.5
84	1297.5
85	855.5
86	633.5
87	490.5
88	385.0
89	341.5
90	299.5
91	251.5
92	228.5
93	203.5
94	177.5
95	160.5
96	124.0
97	92.0
98	80.0
99	57.5
100	35.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.20994999999999997
2	0.15915
3	0.15287499999999998
4	0.14495
5	0.14785
6	0.10464999999999999
7	0.12332499999999999
8	0.14712499999999998
9	0.126025
10-14	0.070535
15-19	0.05212
20-24	0.02571
25-29	0.032654999999999997
30-34	0.059555000000000004
35-39	0.028564999999999997
40-44	0.01963
45-49	0.009025
50-54	0.018695
55-59	0.020935
60-64	5.9999999999999995E-5
65-69	0.0
70-74	0.0
75-79	0.0017
80-84	0.003045
85-89	9.999999999999999E-5
90-94	0.0
95-99	0.004745
100-101	0.0043
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.612592625903769
#Duplication Level	Relative count
1	100.0
2	0.5329123660806306
3	0.007541212727556094
4	0.0025137375758520313
5	0.0015082425455112188
6	0.0010054950303408125
7	0.0
8	5.027475151704062E-4
9	0.0010054950303408125
10++	0.0025137375758520313
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	439445	3.8301125	4.0780144	95-97
AAAAA	444040	3.8018138	4.0702744	85-89
>>END_MODULE