##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R1_005.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	124585	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.434281815627884	34.0	31.0	34.0	31.0	34.0
2	32.71934020949553	34.0	31.0	34.0	31.0	34.0
3	32.777990929887224	34.0	31.0	34.0	31.0	34.0
4	36.262792471003735	37.0	37.0	37.0	35.0	37.0
5	36.10027691937232	37.0	35.0	37.0	35.0	37.0
6	36.1025645141871	37.0	36.0	37.0	35.0	37.0
7	36.07665449291648	37.0	36.0	37.0	35.0	37.0
8	36.100549825420394	37.0	36.0	37.0	35.0	37.0
9	37.82703375205683	39.0	38.0	39.0	35.0	39.0
10-14	38.082729060480794	39.4	38.2	39.4	35.2	39.4
15-19	39.118903559818605	40.4	38.8	41.0	35.8	41.0
20-24	38.9362202512341	40.0	38.4	41.0	35.0	41.0
25-29	38.421073162900825	40.0	38.0	41.0	33.8	41.0
30-34	37.75376971545531	39.8	36.6	41.0	33.0	41.0
35-39	36.57145723803026	38.4	35.0	40.2	31.0	41.0
40-44	35.313015210498854	36.4	34.6	39.8	28.6	41.0
45-49	35.151981378175535	35.0	34.8	39.0	30.8	41.0
50-54	33.65143476341454	35.0	33.4	36.8	26.0	40.2
55-59	33.46980936709876	35.0	33.0	35.0	27.6	39.0
60-64	33.27047236826264	35.0	33.0	35.0	27.6	37.2
65-69	32.87819560942329	35.0	33.0	35.0	27.8	35.8
70-74	32.599526427740095	35.0	33.0	35.0	27.2	35.0
75-79	31.851100854838062	34.4	32.4	35.0	26.2	35.0
80-84	32.077383312597824	35.0	33.0	35.0	27.0	35.0
85-89	31.771081590881728	35.0	33.0	35.0	25.6	35.0
90-94	31.310584741341252	34.6	32.2	35.0	24.2	35.0
95-99	30.763175342135895	34.0	31.6	35.0	22.8	35.0
100-101	29.204438736605532	33.5	29.0	34.5	10.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	4.0
5	6.0
6	9.0
7	27.0
8	81.0
9	120.0
10	170.0
11	185.0
12	190.0
13	195.0
14	233.0
15	215.0
16	243.0
17	257.0
18	261.0
19	289.0
20	314.0
21	371.0
22	458.0
23	559.0
24	711.0
25	936.0
26	1171.0
27	1410.0
28	1856.0
29	2285.0
30	2900.0
31	3568.0
32	4663.0
33	6482.0
34	10596.0
35	23089.0
36	38159.0
37	17514.0
38	4641.0
39	414.0
40	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.124822376185165	21.83749327638667	17.887621326097253	28.15006302133091
2	30.061403860817915	22.28839747963238	22.32371473291327	25.326483926636435
3	27.622105389894447	21.59810571096039	22.8606975157523	27.919091383392864
4	30.1320383673797	20.80105951759843	21.098848175944134	27.968053939077738
5	30.844804751775897	20.27932736685797	19.989565356985192	28.886302524380948
6	29.29245093711121	20.053778544768633	19.143556607938354	31.510213910181804
7	31.513424569570976	19.348236143998072	19.589035598185976	29.549303688244972
8	29.306898904362484	19.715856644058274	19.852309668098087	31.124934783481155
9	29.970702733073807	18.944495725809688	20.33551390616848	30.749287634948026
10-14	30.221294698398683	19.57234016936228	19.80382871132159	30.402536420917446
15-19	30.193522494682345	19.60284143355942	19.81281855761127	30.390817514146967
20-24	30.10096372273426	19.67644730990838	19.83425831236946	30.388330654987904
25-29	30.331678233104252	19.758782385623906	19.67626817439716	30.233271206874683
30-34	30.28262109177355	19.593616730783133	19.869732477733248	30.254029699710074
35-39	30.313000806107144	19.787200560099944	19.76295311411073	30.136845519682183
40-44	30.815015815417222	19.65382700984249	19.597630095855877	29.93352707888441
45-49	30.21262591804792	19.58165108159088	19.816350282939357	30.389372717421843
50-54	30.781394228839748	19.713127583577478	19.66352289601477	29.841955291568006
55-59	30.33671790343942	19.687923907372475	19.644098406710278	30.331259782477826
60-64	30.288897629578493	19.60249484254718	19.70684797354247	30.401759554331857
65-69	30.26955000080271	19.713914174252274	19.819711345502416	30.196824479442597
70-74	30.395633503230723	19.610707549062887	19.700927077898623	30.29273186980776
75-79	30.22883974796324	19.807681502588594	19.714732913272062	30.248745836176106
80-84	30.310711562387127	19.75759521611751	19.80800256852751	30.123690652967856
85-89	30.417305454107634	19.698198017417827	19.68294738531926	30.201549143155276
90-94	30.267363881713194	19.614872707948347	19.77381718815976	30.343946222178698
95-99	30.299634787494483	19.68519484689168	19.884255729020346	30.13091463659349
100-101	30.652967853272866	19.684954047437493	19.48549183288518	30.176586266404463
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.27488539751607
2	55.387887787454346
3	55.54119677328732
4	58.10009230645744
5	59.731107276156834
6	60.80266484729301
7	61.06272825781595
8	60.431833687843636
9	60.71999036802184
10-14	60.62383111931613
15-19	60.584340008829315
20-24	60.48929437772216
25-29	60.564949439978946
30-34	60.536650791483616
35-39	60.44984632578932
40-44	60.748542894301636
45-49	60.601998635469755
50-54	60.62334952040776
55-59	60.66797768591724
60-64	60.69065718391036
65-69	60.466374480245314
70-74	60.688365373038486
75-79	60.477585584139334
80-84	60.43440221535498
85-89	60.61885459726292
90-94	60.61131010389189
95-99	60.43054942408798
100-101	60.82955411967733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.0
4	0.5
5	0.5
6	1.0
7	2.0
8	2.0
9	2.5
10	3.5
11	3.5
12	3.5
13	5.0
14	6.5
15	7.5
16	10.5
17	13.5
18	17.5
19	26.0
20	35.5
21	41.0
22	43.5
23	52.0
24	58.5
25	76.5
26	94.5
27	129.0
28	165.0
29	181.0
30	204.0
31	251.0
32	328.5
33	380.5
34	435.0
35	503.5
36	562.0
37	646.5
38	717.5
39	760.5
40	820.5
41	886.0
42	926.5
43	987.0
44	1061.5
45	1110.0
46	1161.5
47	1241.0
48	1347.0
49	1501.5
50	1657.5
51	1843.5
52	2095.5
53	2355.5
54	2665.5
55	2973.5
56	3248.5
57	3610.0
58	3931.5
59	4243.5
60	4565.0
61	4958.0
62	5368.0
63	5647.0
64	5841.5
65	5983.5
66	5965.0
67	5842.5
68	5667.5
69	5269.5
70	4727.5
71	4176.0
72	3649.0
73	3011.0
74	2383.5
75	1869.0
76	1408.5
77	993.5
78	694.0
79	491.5
80	300.5
81	154.0
82	80.0
83	36.5
84	19.0
85	15.0
86	13.0
87	7.0
88	3.5
89	3.5
90	1.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019263956335032306
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0044949231448408716
25-29	3.210659389172051E-4
30-34	0.057791869005096926
35-39	0.028735401533089858
40-44	0.018461291487739295
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.006421318778344102
65-69	0.00561865393105109
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.010595175984267768
95-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	124585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.056186539310510895
#Duplication Level	Relative count
1	100.0
2	0.025699714893787896
3	0.0
4	0.0
5	0.0
6	8.031160904308718E-4
7	0.0
8	0.0
9	0.0
10++	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13100	3.5793009	4.6374035	9
AAAAA	12675	3.5211494	4.5803146	95-97
>>END_MODULE