##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R1_003.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.714975	34.0	31.0	34.0	31.0	34.0
2	32.96042	34.0	31.0	34.0	31.0	34.0
3	32.93980875	34.0	31.0	34.0	31.0	34.0
4	36.39229925	37.0	37.0	37.0	35.0	37.0
5	36.339397	37.0	37.0	37.0	35.0	37.0
6	36.3203615	37.0	37.0	37.0	35.0	37.0
7	36.32596775	37.0	37.0	37.0	35.0	37.0
8	36.2815855	37.0	37.0	37.0	35.0	37.0
9	38.09736275	39.0	39.0	39.0	37.0	39.0
10-14	38.1686189	39.4	38.4	39.4	36.0	39.4
15-19	39.3389132	41.0	39.0	41.0	36.4	41.0
20-24	39.18441505	40.8	39.0	41.0	35.8	41.0
25-29	37.664529200000004	40.0	37.2	41.0	31.2	41.0
30-34	35.0556907	38.6	33.8	40.2	23.0	41.0
35-39	36.098233199999996	38.0	35.0	40.0	29.4	41.0
40-44	31.9657064	35.8	25.4	39.6	18.0	41.0
45-49	33.63471475	35.0	32.2	38.4	25.6	41.0
50-54	33.8329585	35.0	33.8	37.0	27.6	40.4
55-59	32.55264345	35.0	33.0	35.0	23.2	39.0
60-64	33.128449	35.0	33.0	35.0	27.4	37.2
65-69	32.37121915	35.0	33.0	35.0	25.8	35.8
70-74	31.82325965	35.0	32.6	35.0	23.8	35.0
75-79	31.710977550000003	34.4	32.4	35.0	25.2	35.0
80-84	32.055303800000004	35.0	33.0	35.0	26.4	35.0
85-89	31.7086059	35.0	33.0	35.0	25.6	35.0
90-94	31.437360050000002	35.0	33.0	35.0	24.0	35.0
95-99	30.66904725	35.0	32.2	35.0	18.8	35.0
100-101	28.777226	33.5	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76.0
3	15.0
4	54.0
5	144.0
6	420.0
7	1324.0
8	3504.0
9	5876.0
10	6861.0
11	7546.0
12	7680.0
13	7253.0
14	7201.0
15	7551.0
16	7965.0
17	8292.0
18	9082.0
19	9856.0
20	10679.0
21	12234.0
22	14537.0
23	18578.0
24	23902.0
25	30450.0
26	38904.0
27	50557.0
28	65375.0
29	84432.0
30	110552.0
31	151847.0
32	226291.0
33	352675.0
34	527389.0
35	722724.0
36	895425.0
37	441407.0
38	117926.0
39	13334.0
40	82.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.100915605095544	21.688183153026277	17.751301291489295	28.459599950388885
2	30.254625	22.356675	22.1944	25.1943
3	27.93555	21.482950000000002	22.709625	27.871875000000003
4	30.168	20.54745	20.827725	28.456825000000002
5	30.724325000000004	20.406525000000002	19.74525	29.123900000000003
6	29.2437	20.030075	19.176625	31.549599999999998
7	31.604	19.367124999999998	19.487975	29.540899999999997
8	29.225025	19.687675	20.0329	31.054399999999998
9	29.721874999999997	18.967325	20.463725	30.847075
10-14	30.395891519794578	19.6175459808773	19.7165059858253	30.270056513502823
15-19	30.24634	19.618305	19.722585000000002	30.412770000000002
20-24	30.288812025329243	19.704797863482824	19.812874744020544	30.19351536716739
25-29	30.811388371190553	19.61502377606671	19.61580380286063	29.957784049882115
30-34	31.295155057691414	19.586182998843217	19.34363133356008	29.77503060990529
35-39	30.730326256422693	19.734269786764507	19.65965596828533	29.875747988527472
40-44	33.66124751553904	19.092298439189193	18.679575883937304	28.566878161334465
45-49	30.526395	19.68743	19.709004999999998	30.07717
50-54	30.594422945817207	19.850754888066167	19.670484752863565	29.88433741325306
55-59	30.70363114072623	19.860258972051795	19.56125391225078	29.874855974971194
60-64	30.39899305348928	19.706457289535628	19.669181865961498	30.22536779101359
65-69	30.415723147533825	19.90507169078562	19.690373374548475	29.98883178713208
70-74	30.615773603539793	19.806040234966137	19.560608001532813	30.017578159961257
75-79	30.419439999999998	19.684965	19.668385	30.22721
80-84	30.292472795911447	19.744829996915474	19.746090006806554	30.216607200366525
85-89	30.46787362641127	19.724517429706097	19.64795004478182	30.159658899100815
90-94	30.37505670993558	19.62858392790417	19.68416366547419	30.31219569668606
95-99	30.334754874627297	19.72292544838251	19.738610536219003	30.20370914077119
100-101	30.935000000000002	19.774449999999998	19.351725000000002	29.938825
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.56051555548443
2	55.448925
3	55.807425
4	58.624825
5	59.848225
6	60.793299999999995
7	61.1449
8	60.279424999999996
9	60.56895
10-14	60.665948033297404
15-19	60.659110000000005
20-24	60.48232739249663
25-29	60.76917242107266
30-34	61.07018566759671
35-39	60.60607424495017
40-44	62.2281256768735
45-49	60.603565
50-54	60.47876035907027
55-59	60.57848711569742
60-64	60.62436084450288
65-69	60.404554934665896
70-74	60.633351763501054
75-79	60.64665
80-84	60.50907999627797
85-89	60.62753252551209
90-94	60.68725240662164
95-99	60.53846401539849
100-101	60.873825000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	31.0
2	29.0
3	26.0
4	26.0
5	32.0
6	32.5
7	36.0
8	44.0
9	61.0
10	76.0
11	100.0
12	140.0
13	169.5
14	213.5
15	268.5
16	333.0
17	443.0
18	531.5
19	648.5
20	829.0
21	1011.5
22	1231.5
23	1506.5
24	1899.0
25	2422.5
26	2903.5
27	3514.5
28	4394.0
29	5382.0
30	6436.0
31	7717.0
32	9379.0
33	11141.5
34	13043.5
35	15022.0
36	17023.0
37	19382.0
38	21798.0
39	24061.5
40	26074.5
41	28008.0
42	29718.5
43	31451.0
44	33703.0
45	36143.0
46	38474.0
47	41174.0
48	44971.5
49	49465.0
50	54883.0
51	61212.5
52	68072.5
53	76074.5
54	84797.5
55	94127.0
56	104178.0
57	114759.0
58	126015.0
59	137867.0
60	149245.0
61	160500.5
62	170679.5
63	179583.0
64	187291.0
65	191058.5
66	191260.5
67	188356.0
68	180057.5
69	167384.5
70	152348.0
71	134910.5
72	116091.5
73	96538.5
74	77847.0
75	60814.0
76	45794.5
77	33260.0
78	23116.5
79	15203.0
80	9533.0
81	5826.5
82	3394.5
83	1916.0
84	1112.0
85	720.5
86	496.5
87	340.0
88	244.5
89	185.0
90	125.0
91	83.0
92	52.5
93	38.5
94	33.0
95	21.0
96	9.5
97	3.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0224
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.9999999999999996E-6
15-19	0.0
20-24	0.00174
25-29	0.003435
30-34	0.755165
35-39	0.018520000000000002
40-44	0.7153849999999999
45-49	0.0
50-54	7.500000000000001E-5
55-59	1.9999999999999998E-5
60-64	0.01455
65-69	0.01086
70-74	9.099999999999999E-4
75-79	0.0
80-84	7.849999999999999E-4
85-89	0.009645
90-94	0.01752
95-99	5.6E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.30293184033592696
#Duplication Level	Relative count
1	100.0
2	0.27326651256775253
3	0.002507032225392225
4	0.002507032225392225
5	0.0015042193352353352
6	5.01406445078445E-4
7	0.0
8	0.0
9	0.0
10++	0.00100281289015689
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	418745	3.6756177	4.3667874	95-97
AAAAA	417105	3.5730066	4.001713	85-89
>>END_MODULE