##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R1_002.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85359775	34.0	31.0	34.0	31.0	34.0
2	33.07768475	34.0	33.0	34.0	31.0	34.0
3	33.106995	34.0	33.0	34.0	31.0	34.0
4	36.47641225	37.0	37.0	37.0	35.0	37.0
5	36.4153925	37.0	37.0	37.0	35.0	37.0
6	36.38110975	37.0	37.0	37.0	35.0	37.0
7	36.37473975	37.0	37.0	37.0	35.0	37.0
8	36.36294225	37.0	37.0	37.0	35.0	37.0
9	38.1547785	39.0	39.0	39.0	37.0	39.0
10-14	38.426328	39.4	39.0	39.4	37.0	39.4
15-19	39.567803	41.0	40.0	41.0	37.0	41.0
20-24	39.355499699999996	41.0	39.2	41.0	36.2	41.0
25-29	38.91739460000001	40.0	38.4	41.0	35.2	41.0
30-34	38.209874150000005	40.0	37.4	41.0	34.0	41.0
35-39	37.239606300000005	38.8	35.2	40.6	32.8	41.0
40-44	36.41817605	37.2	35.0	40.0	32.4	41.0
45-49	35.822783099999995	35.2	35.0	39.4	32.8	41.0
50-54	34.98590695	35.0	35.0	37.6	31.6	40.6
55-59	34.3306793	35.0	34.4	35.4	31.0	39.4
60-64	33.820670899999996	35.0	34.0	35.0	30.6	37.6
65-69	33.399647	35.0	33.6	35.0	29.8	36.2
70-74	33.07967935	35.0	33.0	35.0	29.0	35.2
75-79	32.3862981	34.4	32.6	35.0	28.0	35.0
80-84	32.64808910000001	35.0	33.0	35.0	29.0	35.0
85-89	32.3223621	35.0	33.0	35.0	27.4	35.0
90-94	31.92596585	35.0	33.0	35.0	27.0	35.0
95-99	31.38928445	35.0	33.0	35.0	24.6	35.0
100-101	30.1000775	34.0	31.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	14.0
4	44.0
5	119.0
6	340.0
7	850.0
8	1962.0
9	2880.0
10	3708.0
11	4313.0
12	4368.0
13	4608.0
14	4992.0
15	5254.0
16	5790.0
17	6518.0
18	7112.0
19	7679.0
20	8326.0
21	9415.0
22	11049.0
23	13948.0
24	16858.0
25	21506.0
26	27139.0
27	34435.0
28	42687.0
29	53231.0
30	67062.0
31	85940.0
32	113650.0
33	159694.0
34	263339.0
35	657323.0
36	1371577.0
37	747403.0
38	210616.0
39	24073.0
40	133.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.176823445841386	21.627740035059848	17.69857182755516	28.496864691543607
2	30.2979	22.338975	22.104025	25.2591
3	27.6738	21.482875	22.73835	28.104975
4	30.190675	20.497975	20.818025000000002	28.493325000000002
5	30.814075000000003	20.336375	19.6949	29.15465
6	29.28715	20.000525	19.127875	31.584449999999997
7	31.65095	19.307725	19.454475	29.58685
8	29.236375	19.62865	19.980325	31.15465
9	29.8035	18.93245	20.441125	30.822925
10-14	30.30851530851531	19.590044590044588	19.735479735479736	30.365960365960365
15-19	30.249955	19.602339999999998	19.723685	30.424020000000002
20-24	30.153100553585194	19.68375688931491	19.83905694366993	30.324085613429965
25-29	30.332734141201	19.68594165252023	19.70046226964646	30.28086193663231
30-34	30.27309148421386	19.63776313920602	19.717945699491654	30.371199677088466
35-39	30.239473943468624	19.704430763045018	19.804029039328714	30.25206625415764
40-44	30.359534953652417	19.66384796607099	19.65850287012652	30.31811421015007
45-49	30.280325	19.620124999999998	19.712655	30.386895000000003
50-54	30.222943579653304	19.70013122034117	19.788976451338776	30.28794874866675
55-59	30.38134	19.657275	19.64911	30.312275
60-64	30.297598363392552	19.616298095899417	19.711649164881678	30.37445437582635
65-69	30.23550175841973	19.73757316621031	19.756058367021563	30.270866708348393
70-74	30.383905	19.684045	19.639200000000002	30.292849999999998
75-79	30.31417	19.63307	19.68046	30.372300000000003
80-84	30.274955948935116	19.70952073531464	19.753903067606203	30.261620248144038
85-89	30.42756040410886	19.664411865330454	19.617406761583403	30.290620968977283
90-94	30.36074695392637	19.603453959132917	19.669294867530375	30.366504219410338
95-99	30.34155015204104	19.689941188327918	19.725670545523027	30.242838114108018
100-101	30.70524444846671	19.591090246424116	19.427063339611323	30.27660196549785
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.67368813738499
2	55.557
3	55.778775
4	58.684000000000005
5	59.968725
6	60.8716
7	61.2378
8	60.391025
9	60.626425
10-14	60.67447567447567
15-19	60.673975
20-24	60.47718616701516
25-29	60.61359607783331
30-34	60.64429116130232
35-39	60.49154019762626
40-44	60.67764916380249
45-49	60.66722
50-54	60.510892328320054
55-59	60.693615
60-64	60.672052739218906
65-69	60.50636846676812
70-74	60.67675500000001
75-79	60.68647
80-84	60.536576197079164
85-89	60.718181373086146
90-94	60.727251173336704
95-99	60.584388266149055
100-101	60.98184641396456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	46.0
2	28.0
3	25.5
4	24.0
5	32.0
6	34.0
7	37.5
8	52.0
9	64.0
10	86.0
11	105.5
12	132.0
13	191.0
14	234.5
15	280.5
16	356.0
17	428.0
18	559.0
19	718.0
20	871.0
21	1067.5
22	1333.5
23	1617.5
24	1982.0
25	2519.0
26	3124.5
27	3822.0
28	4701.5
29	5656.5
30	6763.0
31	8201.0
32	9939.0
33	11691.5
34	13511.0
35	15646.0
36	17795.0
37	20016.0
38	22240.5
39	24120.0
40	25992.0
41	27968.5
42	29765.5
43	31697.5
44	33638.5
45	35721.0
46	38265.5
47	41459.5
48	45140.5
49	49288.5
50	54524.0
51	60949.0
52	68311.5
53	76193.0
54	84874.0
55	94169.5
56	104077.0
57	114608.0
58	125732.5
59	136740.0
60	147406.0
61	158586.5
62	169275.5
63	178291.5
64	185159.0
65	189490.5
66	190187.0
67	186716.5
68	179528.5
69	168178.0
70	152871.5
71	135349.5
72	116816.5
73	97976.0
74	79514.5
75	62218.0
76	46924.5
77	33876.0
78	23428.5
79	15593.5
80	9838.0
81	5844.5
82	3264.5
83	1775.5
84	987.5
85	560.5
86	352.5
87	236.5
88	173.5
89	125.0
90	88.5
91	70.5
92	42.5
93	21.5
94	14.5
95	7.5
96	5.5
97	3.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013825
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	9.999999999999999E-5
15-19	0.0
20-24	3.5E-5
25-29	0.00425
30-34	0.059315
35-39	0.018349999999999998
40-44	0.001795
45-49	0.0
50-54	2.6000000000000003E-4
55-59	0.0
60-64	0.027339999999999996
65-69	0.028134999999999997
70-74	0.0
75-79	0.0
80-84	0.005254999999999999
85-89	0.021495
90-94	0.03935
95-99	0.012195
100-101	0.0011625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5613231831972837
#Duplication Level	Relative count
1	100.0
2	0.5252840052277068
3	0.002513320599175631
4	0.0020106564793405048
5	5.026641198351262E-4
6	5.026641198351262E-4
7	0.0
8	0.0010053282396702524
9	0.0
10++	0.0010053282396702524
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	417025	3.5788107	3.8649764	90-94
AAAAA	413345	3.5585952	3.7998223	65-69
>>END_MODULE