##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	002003_Wally_GTTTCG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4000000	
Filtered Sequences	0	
Sequence length	101	
%GC	60	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76561825	34.0	31.0	34.0	31.0	34.0
2	33.0107635	34.0	31.0	34.0	31.0	34.0
3	33.03917175	34.0	31.0	34.0	31.0	34.0
4	36.42251	37.0	37.0	37.0	35.0	37.0
5	36.3584635	37.0	37.0	37.0	35.0	37.0
6	36.32184525	37.0	37.0	37.0	35.0	37.0
7	36.3150695	37.0	37.0	37.0	35.0	37.0
8	36.304396	37.0	37.0	37.0	35.0	37.0
9	38.08966875	39.0	39.0	39.0	37.0	39.0
10-14	38.3585425	39.4	38.6	39.4	35.8	39.4
15-19	39.50125575	41.0	39.8	41.0	37.0	41.0
20-24	39.2861037	40.8	39.0	41.0	36.0	41.0
25-29	38.84863094999999	40.0	38.4	41.0	35.0	41.0
30-34	38.1260254	40.0	37.2	41.0	33.6	41.0
35-39	37.1689502	38.8	35.2	40.6	32.6	41.0
40-44	36.36846985	37.2	35.0	40.0	32.0	41.0
45-49	35.7072206	35.2	35.0	39.4	32.0	41.0
50-54	34.8493395	35.0	35.0	37.6	31.0	40.6
55-59	34.1838868	35.0	34.2	35.4	30.8	39.4
60-64	33.6484684	35.0	34.0	35.0	30.0	37.6
65-69	33.19848315	35.0	33.0	35.0	29.0	36.2
70-74	32.870846549999996	35.0	33.0	35.0	29.0	35.2
75-79	32.11211085	34.4	32.4	35.0	26.8	35.0
80-84	32.399388699999996	35.0	33.0	35.0	27.2	35.0
85-89	32.04734575	35.0	33.0	35.0	27.0	35.0
90-94	31.629726299999998	35.0	33.0	35.0	25.0	35.0
95-99	31.07813245	35.0	32.8	35.0	23.8	35.0
100-101	29.878754	34.0	30.5	35.0	17.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61.0
3	17.0
4	42.0
5	146.0
6	334.0
7	923.0
8	2254.0
9	3415.0
10	4335.0
11	4803.0
12	5190.0
13	5471.0
14	5805.0
15	6271.0
16	6893.0
17	7683.0
18	8533.0
19	8868.0
20	9852.0
21	10892.0
22	12577.0
23	15433.0
24	19172.0
25	23738.0
26	30172.0
27	37433.0
28	46582.0
29	57943.0
30	73146.0
31	92626.0
32	121683.0
33	168940.0
34	271925.0
35	640199.0
36	1315234.0
37	741186.0
38	214223.0
39	25814.0
40	186.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.10719320591507	21.680054479926035	17.767498462562685	28.44525385159622
2	30.28035	22.354325	22.178875	25.18645
3	27.674425000000003	21.5477	22.773075000000002	28.004800000000003
4	30.16355	20.484175	20.8616	28.490675
5	30.78575	20.368075	19.722224999999998	29.123949999999997
6	29.253425	20.0169	19.203924999999998	31.525750000000002
7	31.627475	19.3854	19.524475	29.46265
8	29.177500000000002	19.6774	20.079150000000002	31.065949999999997
9	29.717325	18.94765	20.490924999999997	30.8441
10-14	30.271047191170336	19.634580068179176	19.766905405608785	30.327467335041703
15-19	30.19337	19.643115	19.77396	30.389555
20-24	30.14336081175143	19.725670489884983	19.86218173896291	30.26878695940068
25-29	30.30241742338439	19.713527598938636	19.73436262810768	30.24969234956929
30-34	30.237052154655547	19.6452289049985	19.778257685647382	30.339461254698563
35-39	30.192694826284526	19.748764454287183	19.82835223001287	30.230188489415415
40-44	30.358037950053696	19.663164635816774	19.692499754624006	30.28629765950552
45-49	30.24769	19.635675	19.76749	30.349145
50-54	30.23829078882354	19.724373617540518	19.78500958165235	30.252326011983588
55-59	30.371575	19.66366	19.687569999999997	30.277195000000003
60-64	30.284038441208573	19.647776610607487	19.708000397257962	30.36018455092598
65-69	30.233726732784177	19.743326424525844	19.783899102905224	30.239047739784752
70-74	30.401280000000003	19.67914	19.65754	30.262040000000002
75-79	30.295075	19.65934	19.702865	30.34272
80-84	30.262338449516374	19.73839408385052	19.766255352931324	30.23301211370178
85-89	30.424153382208107	19.678482640087832	19.639432495231247	30.257931482472816
90-94	30.36968718053793	19.632260755117883	19.671704576728587	30.326347487615607
95-99	30.362551632400407	19.71805906677602	19.710583538256156	30.208805762567415
100-101	30.735175697611655	19.61517701762189	19.439225653998818	30.210421630767637
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	60.55244705751128
2	55.466800000000006
3	55.679225
4	58.654225000000004
5	59.9097
6	60.779174999999995
7	61.09012499999999
8	60.243449999999996
9	60.561425
10-14	60.59851452621204
15-19	60.582924999999996
20-24	60.4121477711521
25-29	60.55210977295368
30-34	60.57651340935411
35-39	60.42288331569995
40-44	60.64433560955922
45-49	60.596835000000006
50-54	60.49061680080713
55-59	60.64877
60-64	60.64422299213456
65-69	60.472774472568936
70-74	60.66332
75-79	60.637795000000004
80-84	60.49535056321815
85-89	60.68208486468092
90-94	60.69603466815353
95-99	60.571357394967826
100-101	60.9455973283793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	49.0
2	29.5
3	23.0
4	18.0
5	30.5
6	43.5
7	49.0
8	59.0
9	72.5
10	90.5
11	117.5
12	144.0
13	179.0
14	220.5
15	287.0
16	380.5
17	475.0
18	599.0
19	729.5
20	894.5
21	1125.0
22	1379.5
23	1679.5
24	2075.5
25	2595.5
26	3121.5
27	3834.0
28	4706.5
29	5754.0
30	6999.5
31	8315.0
32	9947.0
33	11918.5
34	13871.5
35	15849.0
36	18066.5
37	20236.0
38	22304.0
39	24538.0
40	26625.5
41	28201.0
42	30032.0
43	31937.0
44	33888.0
45	36160.0
46	38645.0
47	41729.0
48	45453.0
49	49617.5
50	54910.5
51	61274.0
52	68351.0
53	76260.5
54	84801.5
55	94045.0
56	103979.5
57	114616.5
58	125097.5
59	136167.5
60	147890.0
61	159175.5
62	169502.5
63	178235.5
64	185348.0
65	189213.5
66	189670.5
67	186659.5
68	178781.5
69	166873.5
70	152215.5
71	134957.0
72	116143.5
73	97155.0
74	78642.0
75	61446.5
76	46362.5
77	33710.0
78	23337.0
79	15490.5
80	9883.5
81	5954.5
82	3439.5
83	1960.5
84	1139.0
85	691.5
86	454.5
87	326.0
88	226.0
89	160.5
90	111.5
91	73.5
92	54.0
93	32.5
94	19.5
95	14.0
96	9.5
97	5.5
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036575
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.55E-4
15-19	0.0
20-24	9.15E-4
25-29	1.4E-4
30-34	0.047945
35-39	0.009770000000000001
40-44	4.0500000000000003E-4
45-49	0.0
50-54	0.00159
55-59	0.0
60-64	0.014589999999999999
65-69	0.018925
70-74	0.0
75-79	0.0
80-84	0.004555
85-89	0.013175000000000001
90-94	0.022365
95-99	0.00707
100-101	7.750000000000001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	0.5593294045316172
#Duplication Level	Relative count
1	100.0
2	0.5142202249901981
3	0.0070372269304621446
4	0.0040212725316926545
5	0.0020106362658463273
6	0.0
7	0.0025132953323079086
8	0.0
9	5.026590664615818E-4
10++	0.0015079771993847452
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	420730	3.5764482	3.8251214	85-89
AAAAA	411415	3.5280893	3.8581088	95-97
>>END_MODULE